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  3. Keap1-Nrf2 Environmental Pollutants Endogenous Metabolite Apoptosis
  4. L-Methionine

L-Methionine is an L-isomer of orally active Methionine, an essential amino acid. Methionine is a strong liver antidote that acts as a liver protector. L-Methionine can inhibit cell proliferation and induce cell apoptosis. L-Methionine has antitumor and antioxidant activity.

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CAS No. : 63-68-3

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Based on 10 publication(s) in Google Scholar

Other Forms of L-Methionine:

L-Methionine Related Antibodies

Top Publications Citing Use of Products

    L-Methionine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Mar 24:e2501623.  [Abstract]

    The expression and secretion of PCSK9 in human with methionine deprivation for 6 h and then methionine (0-50 μM) supplementation for 24h. Immunoblotting analysis of PCSK9 in human CRC cell lines (SW480, SW620, HCT116, and LoVo).

    L-Methionine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Mar 24:e2501623.  [Abstract]

    L-Methionine (0, 20, 50 μM). ELISA analysis of PCSK9 in culture supernatant of human CRC cell lines (SW480, SW620, HCT116, and LoVo).

    L-Methionine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Mar 24:e2501623.  [Abstract]

    Mice were housed on either a normal diet (ND) with 0.86% methionine or a dietary methionine restriction (DMR) diet with 0.12% methionine from the day after injection until the endpoints. Immunohistochemistry analysis of PCSK9 in tumors from MC38‐ (n = 9) and Colon 26‐ (n = 4 (ND), n = 8 (DMR)) bearing mice fed with normal diet and methionine restriction diet (left). The abundance of PCSK9 was assessed (right).

    L-Methionine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Mar 24:e2501623.  [Abstract]

    Mice were housed on either a normal diet (ND) with 0.86% methionine or a dietary methionine restriction (DMR) diet with 0.12% methionine from the day after injection until the endpoints. Tumor volume of PCSK9‐KD (n = 10) or PCSK9‐KO (n = 9) MC38‐bearing mice fed with normal diet and methionine restriction diet. EAA, essential amino acid; NEEA, non‐essential amino acid; CM, complete medium; ND, normal diet; DMR, dietary methionine restriction.

    L-Methionine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Mar 24:e2501623.  [Abstract]

    Mice were housed on either a normal diet (ND) with 0.86% methionine or a dietary methionine restriction (DMR) diet with 0.12% methionine from the day after injection until the endpoints. Flow cytometric analysis of TNFα and Granzyme B in intratumoral CD8+T cells from Colon 26‐bearing mice fed with a methionine restriction diet alone or in combination with anti‐PD‐1 therapy.

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    Description

    L-Methionine is an L-isomer of orally active Methionine, an essential amino acid. Methionine is a strong liver antidote that acts as a liver protector. L-Methionine can inhibit cell proliferation and induce cell apoptosis. L-Methionine has antitumor and antioxidant activity[1][2][3].

    IC50 & Target

    Microbial Metabolite

     

    Human Endogenous Metabolite

     

    In Vitro

    L-Methionine (5 mg/mL, 7 or 3 days) can inhibit the proliferation of BXPC-3 (mutant p53) and HPAC (wild-type p53) pancreatic cancer cells, interfere with cell cycle, and induce apoptosis of BXPC-3 cells[1].
    L-Methionine (0.3-10 mM, 24 h) reduces the formation of free radicals in endothelial cells by inducing heme oxidase-1 and ferritin[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    L-Methionine Related Antibodies

    Cell Proliferation Assay[1]

    Cell Line: BXPC-3, HPAC
    Concentration: 5 mg/mL
    Incubation Time: 7 or 3 days
    Result: Reduced growth of BXPC-3 cells by 31 and 32%, respectively.
    Reduced growth of HPAC cells by 35% and 18%, respectively.
    Reduced S to G2 transition in BXPC-3 cells and intervened in both G1–S and S–G2 transitions in HPAC cells.
    Increased late cell apoptosis at a percentage of 76% in BXPC-3 cells.

    Western Blot Analysis[2]

    Cell Line: ECV304
    Concentration: 0.3, 1, 3, 5, 10 mM
    Incubation Time: 24 h
    Result: Increased the level of HO-1 protein up to 9.5-fold and the HO-1 activity.
    In Vivo

    L-Methionine (215, 268.8, 322.5, 430 mg/kg, orally, for 14 consecutive days) induces endogenous antioxidant activity by activating the Nrf2-ARE pathway and inhibits ROS-induced oxidative stress in growing rats[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male Wistar rats (body weight 70-80 g)[3]
    Dosage: 215,268.8, 322.5, 430 mg/kg
    Administration: p.o.
    Result: Reduced the hepatic ROS levels and the hepatic MDA contents.
    Increased the hepatic T-AOC, CAT and T-SOD.
    Increased the hepatic activity of GCL, GR, GST and GPx.
    Clinical Trial
    Molecular Weight

    149.22

    Formula

    C5H11NO2S
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    N[C@@H](CCSC)C(O)=O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : 10 mg/mL (67.02 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 6.7015 mL 33.5076 mL 67.0151 mL
    5 mM 1.3403 mL 6.7015 mL 13.4030 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 12.5 mg/mL (83.77 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
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    Calculation results:
    Working solution concentration: mg/mL
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.84%

    SDS (393 KB)

    COA (244 KB) HNMR (119 KB) RP-HPLC (246 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 6.7015 mL 33.5076 mL 67.0151 mL 167.5379 mL
    5 mM 1.3403 mL 6.7015 mL 13.4030 mL 33.5076 mL
    10 mM 0.6702 mL 3.3508 mL 6.7015 mL 16.7538 mL
    15 mM 0.4468 mL 2.2338 mL 4.4677 mL 11.1692 mL
    20 mM 0.3351 mL 1.6754 mL 3.3508 mL 8.3769 mL
    25 mM 0.2681 mL 1.3403 mL 2.6806 mL 6.7015 mL
    30 mM 0.2234 mL 1.1169 mL 2.2338 mL 5.5846 mL
    40 mM 0.1675 mL 0.8377 mL 1.6754 mL 4.1884 mL
    50 mM 0.1340 mL 0.6702 mL 1.3403 mL 3.3508 mL
    60 mM 0.1117 mL 0.5585 mL 1.1169 mL 2.7923 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    L-Methionine Related Classifications

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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    L-Methionine63-68-3Keap1-Nrf2Environmental PollutantsEndogenous MetaboliteApoptosisEnvironmental ContaminantsMethionineAntitumorAntioxidantBXPC-3HPACECV304Inhibitorinhibitorinhibit

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