Citric acid monohydrate

Name: Citric acid monohydrate
Brand: MedChemExpress
SKU: HY-N1428A
Rating: 5.0 (8 reviews)

製品番号: HY-N1428A 純度: 98.0%: Data Sheet SDS COA 取扱説明書
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Citric acid monohydrate is a natural preservative and food tartness enhancer. Citric acid monohydrate induces apoptosis and cell cycle arrest at G2/M phase and S phase in HaCaT cells. Citric acid monohydrate cause oxidative damage of the liver by means of the decrease of antioxidative enzyme activities. Citric acid monohydrate is also an acidulant, emulsifier, sequestrant and buffering agent widely used across many industries.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

Citric acid monohydrate 構造式

CAS 番号 : 5949-29-1

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 27	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 27	在庫あり	Estimated Time of Arrival: December 31
Solid
10 g	$25	在庫あり	Estimated Time of Arrival: December 31
25 g	$45	在庫あり	Estimated Time of Arrival: December 31
50 g	$65	在庫あり	Estimated Time of Arrival: December 31
100 g	$95	在庫あり	Estimated Time of Arrival: December 31
> 100 g		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 8 publication(s) in Google Scholar

Other Forms of Citric acid monohydrate:

Ferric citrate 在庫あり
Citric acid 在庫あり
Citric acid monohydrate (Standard) お問い合わせ

顧客検証

Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Human Endogenous Metabolite Microbial Metabolite

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

IC₅₀ & Target

Human Endogenous Metabolite

体外実験

Citric acid (0-12.5 mM; 24 h) monohydrate shows antiproliferative activity in a dose dependent manner^[3].
Citric acid (12.5 mM; 72 h) monohydrate induces apoptosis and cell cycle arrest at G2/M phase and S phase in a dosedependent manner^[3].
Citric acid (12.5 mM; 48 h) monohydrate increases the expression of FAS, BAX, BID, AIF, EndoG, cytochrome c, PARP, GADD153, GRP78 and caspase-3, -8, -9, and decreases of BCL-2 and BCL-Xl^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	HaCaT cells
Concentration:	0, 2.5, 5, 7.5, 10, 12.5 mM
Incubation Time:	24 h
Result:	Inhibited the cell viability in a dose dependent manner.

Cell Cycle Analysis^[3]

Cell Line:	HaCaT cells
Concentration:	12.5 mM
Incubation Time:	0, 12, 24, 48, 72 h
Result:	Induced apoptosis and cell cycle arrest at G2/M phase and S phase in a dosedependent manner.

Western Blot Analysis^[3]

Cell Line:	HaCaT cells
Concentration:	12.5 mM
Incubation Time:	12, 24, 48 h
Result:	Increased the expression of FAS, BAX, BID, AIF, EndoG, cytochrome c, PARP, GADD153, GRP78 and caspase-3, -8, -9, and decreased of BCL-2 and BCL-Xl.

体内実験

Citric acid (120, 240, and 480 mg/kg; i.p.) monohydrate significantly decreases GSH-Px activity and induces an increase in the MDA (malonyldialdehyde) levels in mouse liver^[1].
Citric acid (120, 240, and 480 mg/kg; i.p.) monohydrate induces apoptosis by increases caspase-3 activity in a dose-dependent manner in mouse hepatocytes^[1].
Citric acid (120, 240, and 480 mg/kg; i.p.; weekly for 3 weeks) monohydrate causes renal toxicity in mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	20 g male Kunming mice^[2]
Dosage:	120, 240, 480 mg/kg
Administration:	I.p.; weekly for 3 weeks
Result:	T-SOD and GSH-Px activities in the treated groups decreased with increasing doses of citric acid, NOS activity tended to increase, and H2O2 and MDA contents gradually decreased.

分子量

210.14

分子式

C₆H₁₀O₈

CAS 番号

5949-29-1

Appearance

Solid

Color

White to off-white

SMILES

O=C(CC(C(O)=O)(O)CC(O)=O)O.O

Initial Source

Aspergillus niger

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Store at room temperature, keep dry and cool

*In solvent : -80°C, 6 months; -20°C, 1 month (Store at room temperature, keep dry and cool)

溶剤 & 溶解度

体外:

H₂O : 200 mg/mL (951.75 mM; Need ultrasonic)

DMSO : 100 mg/mL (475.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.7587 mL	23.7937 mL	47.5873 mL
5 mM	0.9517 mL	4.7587 mL	9.5175 mL
10 mM	0.4759 mL	2.3794 mL	4.7587 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (11.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

純度とドキュメンテーション

純度: 98.0%

Select Batch:

データシート (281 KB) SDS (393 KB)

COA (250 KB) HNMR (245 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Chen X, et al. Study on injury effect of food additive citric acid on liver tissue in mice. Cytotechnology. 2014 Mar;66(2):275-82. [Content Brief]

[2]. Chen X, Lv Q, Liu Y, Deng W. Effects of the food additive, citric acid, on kidney cells of mice. Biotech Histochem. 2015 Jan;90(1):38-44. [Content Brief]

[3]. Ying TH, et al. Citric acid induces cell-cycle arrest and apoptosis of human immortalized keratinocyte cell line (HaCaT) via caspase- and mitochondrial-dependent signaling pathways. Anticancer Res. 2013 Oct;33(10):4411-20. [Content Brief]

[4]. Ciriminna R, et al. Citric acid: emerging applications of key biotechnology industrial product. Chem Cent J. 2017 Mar 8;11:22. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	4.7587 mL	23.7937 mL	47.5873 mL	118.9683 mL
	5 mM	0.9517 mL	4.7587 mL	9.5175 mL	23.7937 mL
	10 mM	0.4759 mL	2.3794 mL	4.7587 mL	11.8968 mL
	15 mM	0.3172 mL	1.5862 mL	3.1725 mL	7.9312 mL
	20 mM	0.2379 mL	1.1897 mL	2.3794 mL	5.9484 mL
	25 mM	0.1903 mL	0.9517 mL	1.9035 mL	4.7587 mL
	30 mM	0.1586 mL	0.7931 mL	1.5862 mL	3.9656 mL
	40 mM	0.1190 mL	0.5948 mL	1.1897 mL	2.9742 mL
	50 mM	0.0952 mL	0.4759 mL	0.9517 mL	2.3794 mL
	60 mM	0.0793 mL	0.3966 mL	0.7931 mL	1.9828 mL
	80 mM	0.0595 mL	0.2974 mL	0.5948 mL	1.4871 mL
	100 mM	0.0476 mL	0.2379 mL	0.4759 mL	1.1897 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.