Citric acid monohydrate

Name: Citric acid monohydrate
Brand: MedChemExpress
SKU: HY-N1428A
Rating: 5.0 (8 reviews)

Cat. No.: HY-N1428A Purity: 98.0%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Citric acid monohydrate is a natural preservative and food tartness enhancer. Citric acid monohydrate induces apoptosis and cell cycle arrest at G2/M phase and S phase in HaCaT cells. Citric acid monohydrate cause oxidative damage of the liver by means of the decrease of antioxidative enzyme activities. Citric acid monohydrate is also an acidulant, emulsifier, sequestrant and buffering agent widely used across many industries.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Citric acid monohydrate 화학구조

CAS No. : 5949-29-1

사이즈	재고	수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
10 g	해외재고보유	Estimated Time of Arrival: December 31
25 g	해외재고보유	Estimated Time of Arrival: December 31
50 g	해외재고보유	Estimated Time of Arrival: December 31
100 g	해외재고보유	Estimated Time of Arrival: December 31
> 100 g	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 8 publication(s) in Google Scholar

Other Forms of Citric acid monohydrate:

Ferric citrate In-stock
Citric acid In-stock
Citric acid monohydrate (Standard) 견적 받기

8 Publications Citing Use of MCE Citric acid monohydrate

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

View All Isoforms

Human Endogenous Metabolite Microbial Metabolite

Biological Activity
순도&문서
References
고객리뷰

제품 설명

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

Citric acid (0-12.5 mM; 24 h) monohydrate shows antiproliferative activity in a dose dependent manner^[3].
Citric acid (12.5 mM; 72 h) monohydrate induces apoptosis and cell cycle arrest at G2/M phase and S phase in a dosedependent manner^[3].
Citric acid (12.5 mM; 48 h) monohydrate increases the expression of FAS, BAX, BID, AIF, EndoG, cytochrome c, PARP, GADD153, GRP78 and caspase-3, -8, -9, and decreases of BCL-2 and BCL-Xl^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	HaCaT cells
Concentration:	0, 2.5, 5, 7.5, 10, 12.5 mM
Incubation Time:	24 h
Result:	Inhibited the cell viability in a dose dependent manner.

Cell Cycle Analysis^[3]

Cell Line:	HaCaT cells
Concentration:	12.5 mM
Incubation Time:	0, 12, 24, 48, 72 h
Result:	Induced apoptosis and cell cycle arrest at G2/M phase and S phase in a dosedependent manner.

Western Blot Analysis^[3]

Cell Line:	HaCaT cells
Concentration:	12.5 mM
Incubation Time:	12, 24, 48 h
Result:	Increased the expression of FAS, BAX, BID, AIF, EndoG, cytochrome c, PARP, GADD153, GRP78 and caspase-3, -8, -9, and decreased of BCL-2 and BCL-Xl.

In Vivo

Citric acid (120, 240, and 480 mg/kg; i.p.) monohydrate significantly decreases GSH-Px activity and induces an increase in the MDA (malonyldialdehyde) levels in mouse liver^[1].
Citric acid (120, 240, and 480 mg/kg; i.p.) monohydrate induces apoptosis by increases caspase-3 activity in a dose-dependent manner in mouse hepatocytes^[1].
Citric acid (120, 240, and 480 mg/kg; i.p.; weekly for 3 weeks) monohydrate causes renal toxicity in mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	20 g male Kunming mice^[2]
Dosage:	120, 240, 480 mg/kg
Administration:	I.p.; weekly for 3 weeks
Result:	T-SOD and GSH-Px activities in the treated groups decreased with increasing doses of citric acid, NOS activity tended to increase, and H2O2 and MDA contents gradually decreased.

분자량

210.14

화학식

C₆H₁₀O₈

CAS No.

5949-29-1

Appearance

Solid

Color

White to off-white

SMILES

O=C(CC(C(O)=O)(O)CC(O)=O)O.O

Initial Source

Aspergillus niger

선적

Room temperature in continental US; may vary elsewhere.

보관

Store at room temperature, keep dry and cool

In solvent	-80°C	1 year
	-20°C	6 months

용액&용해도

In Vitro:

H₂O : 200 mg/mL (951.75 mM; Need ultrasonic)

DMSO : 100 mg/mL (475.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.7587 mL	23.7937 mL	47.5873 mL
5 mM	0.9517 mL	4.7587 mL	9.5175 mL
10 mM	0.4759 mL	2.3794 mL	4.7587 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (11.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

순도&문서

Purity: 98.0%

Select Batch:

Data Sheet (281 KB) SDS (393 KB)

COA (250 KB) HNMR (245 KB)

Handling Instructions (2659 KB)

References

[1]. Chen X, et al. Study on injury effect of food additive citric acid on liver tissue in mice. Cytotechnology. 2014 Mar;66(2):275-82. [Content Brief]

[2]. Chen X, Lv Q, Liu Y, Deng W. Effects of the food additive, citric acid, on kidney cells of mice. Biotech Histochem. 2015 Jan;90(1):38-44. [Content Brief]

[3]. Ying TH, et al. Citric acid induces cell-cycle arrest and apoptosis of human immortalized keratinocyte cell line (HaCaT) via caspase- and mitochondrial-dependent signaling pathways. Anticancer Res. 2013 Oct;33(10):4411-20. [Content Brief]

[4]. Ciriminna R, et al. Citric acid: emerging applications of key biotechnology industrial product. Chem Cent J. 2017 Mar 8;11:22. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	4.7587 mL	23.7937 mL	47.5873 mL	118.9683 mL
	5 mM	0.9517 mL	4.7587 mL	9.5175 mL	23.7937 mL
	10 mM	0.4759 mL	2.3794 mL	4.7587 mL	11.8968 mL
	15 mM	0.3172 mL	1.5862 mL	3.1725 mL	7.9312 mL
	20 mM	0.2379 mL	1.1897 mL	2.3794 mL	5.9484 mL
	25 mM	0.1903 mL	0.9517 mL	1.9035 mL	4.7587 mL
	30 mM	0.1586 mL	0.7931 mL	1.5862 mL	3.9656 mL
	40 mM	0.1190 mL	0.5948 mL	1.1897 mL	2.9742 mL
	50 mM	0.0952 mL	0.4759 mL	0.9517 mL	2.3794 mL
	60 mM	0.0793 mL	0.3966 mL	0.7931 mL	1.9828 mL
	80 mM	0.0595 mL	0.2974 mL	0.5948 mL	1.4871 mL
	100 mM	0.0476 mL	0.2379 mL	0.4759 mL	1.1897 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.