2. Autophagy Metabolic Enzyme/Protease
  3. Autophagy Endogenous Metabolite
  4. Guanidine hydrochloride

Guanidine hydrochloride  (Synonyms: Guanidinium chloride; Aminoformamidine hydrochloride)

Cat. No.: HY-B0178A Pureté: 98.0%
COA
SDS
Instruction de manipulation Technical Support

Guanidine hydrochloride (Guanidinium chloride) a strong chaotrope, is also a strong denaturant of proteins.

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CAS No. : 50-01-1

Size Prix Stock Quantité
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in DMSO En stock
Solid
25 g En stock
50 g En stock
100 g En stock
500 g En stock
> 100 g   Obtenir un devis  

* Veuillez sélectionner la quantité avant d'ajouter des articles.

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Based on 3 publication(s) in Google Scholar

Other Forms of Guanidine hydrochloride:

Guanidine hydrochloride Related Antibodies

Top Publications Citing Use of Products

    Guanidine hydrochloride purchased from MedChemExpress. Usage Cited in: Virus Res. 2020 Jul 2;283:197974.  [Abstract]

    RD cells were pretreated with DMSO, NMS-873 (8 μM), DMSO plus GuHCl (2 μM) or NMS-873 plus GuHCl for 30 min. Then cells were transfected with EVA71 replicon RNA (0.1 μg/well) or infected with EVA71 pesudovirus (25 μl/well). Luciferase activity was quantified at different time points post transfection or infection.

    Guanidine hydrochloride purchased from MedChemExpress. Usage Cited in: Virol J. 2018 Jan 3;15(1):1.  [Abstract]

    Immunofluorescence analysis is performed on RD and A549 cells infected with EV71 at an MOI of 10 (in RD) and 50 (in A549) in the presence or absence of 2.5 mM GnHCl.

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    Description

    Guanidine hydrochloride (Guanidinium chloride) a strong chaotrope, is also a strong denaturant of proteins[1][2].

    IC50 & Target

    Human Endogenous Metabolite

     

    In Vitro

    Guanidine hydrochloride at low concentrations refolds acid-unfolded apomyoglobin and cytochrome c, stabilizing the molten globule state, i.e. a compact denatured state with a significant amount of secondary structure, but substantially disordered tertiary structure. Guanidine hydrochloride (> 1 M) causes co-operative unfolding of the molten globule state[1].
    A considerable degree of ordered structure remains due to the presence of disulfide bonds, which Guanidine hydrochloride does not affect[2].
    Guanidine hydrochloride at millimolar concentrations, is able to causes efficient loss of the normally stable [PSI+] element from yeast cells. 5 mM Guanidine hydrochloride in growth media cures [PSI+] and other prions of yeast. 5 mM Guanidine hydrochloride significantly reduces Hsp104-mediated basal and acquired thermotolerance by 30-fold and 50 fold, respectively. Guanidine hydrochloride also reduces the ability of Hsp104 to restore activity of thermally denatured luciferase[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Guanidine hydrochloride Related Antibodies
    In Vivo

    A significant reduction in the death rate of infant mice infected with ten LD50) of coxsackievirus A16 is observed when they are treated 58 h after infection with two injections of Guanidine hydrochloride at 145 mg/kg per injection[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Masse moléculaire

    95.53

    Formule

    CH6ClN3
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    NC(N)=N.Cl
    Structure Classification
    Initial Source
    Livraison

    Room temperature in continental US; may vary elsewhere.
    Stockage

    Store at room temperature, keep dry and cool

    In solvent -80°C 1 year
    -20°C 6 months
    Solvant et solubilité
    In Vitro: 

    DMSO : ≥ 100 mg/mL (1046.79 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 100 mg/mL (1046.79 mM; Need ultrasonic)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 10.4679 mL 52.3396 mL 104.6792 mL
    5 mM 2.0936 mL 10.4679 mL 20.9358 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Calculateur de molarité

    • Calculateur de dilution

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (21.77 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (21.77 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (1046.79 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Pureté et documentation

    Purity: 98.0%

    SDS (394 KB)

    COA (241 KB) HNMR (213 KB)

    Instruction de manipulation (2659 KB)
    Références

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 10.4679 mL 52.3396 mL 104.6792 mL 261.6979 mL
    5 mM 2.0936 mL 10.4679 mL 20.9358 mL 52.3396 mL
    10 mM 1.0468 mL 5.2340 mL 10.4679 mL 26.1698 mL
    15 mM 0.6979 mL 3.4893 mL 6.9786 mL 17.4465 mL
    20 mM 0.5234 mL 2.6170 mL 5.2340 mL 13.0849 mL
    25 mM 0.4187 mL 2.0936 mL 4.1872 mL 10.4679 mL
    30 mM 0.3489 mL 1.7447 mL 3.4893 mL 8.7233 mL
    40 mM 0.2617 mL 1.3085 mL 2.6170 mL 6.5424 mL
    50 mM 0.2094 mL 1.0468 mL 2.0936 mL 5.2340 mL
    60 mM 0.1745 mL 0.8723 mL 1.7447 mL 4.3616 mL
    80 mM 0.1308 mL 0.6542 mL 1.3085 mL 3.2712 mL
    100 mM 0.1047 mL 0.5234 mL 1.0468 mL 2.6170 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Guanidine hydrochloride Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Guanidine50-01-1GuanidiniumAminoformamidineAutophagyEndogenous MetaboliteHsp104denaturantproteinschaotropeunfoldedInhibitorinhibitorinhibit

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