2. Anti-infection
  3. Beta-lactamase Antibiotic Bacterial
  4. Nitrocefin

Nitrocefin is a highly activated, chromogenic cephalosporin derivative. Nitrocefin is a chromogenic β-lactamase substrate. Nitrocefin undergoes a distinctive color change from yellow to red as the amide bond in the β-lactam ring is hydrolyzed by β-lactamase. Nitrocefin is used in competitive inhibition studies in developmental work on β-lactamase-resistant antibiotics.

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No. CAS : 41906-86-9

Tamaño Precio Stock Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Based on 2 publication(s) in Google Scholar

Other Forms of Nitrocefin:

Nitrocefin Related Antibodies

Top Publications Citing Use of Products

2 Publications Citing Use of MCE Nitrocefin

Bio/Physico-chemical Assay

    Nitrocefin purchased from MedChemExpress. Usage Cited in: mBio. 2025 Apr 9;16(4):e0297524.  [Abstract]

    Cell lysates of WT, RP47 (∆sdpA), and RP56 (∆ampG) with and without ampicillin treatment were incubated with Nitrocefin solution (1 mg/mL) for 30 min. M2 buffer was used as a negative control. The appearance of red color product indicates a positive β-lactamase activity, while a consistent yellow color indicates the absence of activity.

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    Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

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    Descripciòn

    Nitrocefin is a highly activated, chromogenic cephalosporin derivative. Nitrocefin is a chromogenic β-lactamase substrate. Nitrocefin undergoes a distinctive color change from yellow to red as the amide bond in the β-lactam ring is hydrolyzed by β-lactamase. Nitrocefin is used in competitive inhibition studies in developmental work on β-lactamase-resistant antibiotics[1][2][3][4].

    IC50 & Target

    β-lactam

     

    In Vitro

    Nitrocefin is hydrolysised by β-Lactamase from Mycobacterium tuberculosis[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Nitrocefin Related Antibodies
    Peso molecular

    516.50

    Fòrmula

    C21H16N4O8S2
    No. CAS
    Appearance

    Solid

    Color

    Yellow to orange

    SMILES

    O=C(C(N12)=C(/C=C/C3=CC=C([N+]([O-])=O)C=C3[N+]([O-])=O)CS[C@]2([H])[C@H](NC(CC4=CC=CS4)=O)C1=O)O
    Envío

    Room temperature in continental US; may vary elsewhere.
    Almacenamiento
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvente y solubilidad
    In Vitro: 

    DMSO : 100 mg/mL (193.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9361 mL 9.6805 mL 19.3611 mL
    5 mM 0.3872 mL 1.9361 mL 3.8722 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Calculadora de molaridad

    • Calculadora de dilución

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

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    C2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    (per animal)

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    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Pureza y Documentación

    Purity: 98.92%

    SDS (467 KB)

    COA (250 KB) HNMR (590 KB) RP-HPLC (240 KB) LCMS (94 KB)

    Instrucciones de manejo (2659 KB)
    Referencias

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.9361 mL 9.6805 mL 19.3611 mL 48.4027 mL
    5 mM 0.3872 mL 1.9361 mL 3.8722 mL 9.6805 mL
    10 mM 0.1936 mL 0.9681 mL 1.9361 mL 4.8403 mL
    15 mM 0.1291 mL 0.6454 mL 1.2907 mL 3.2268 mL
    20 mM 0.0968 mL 0.4840 mL 0.9681 mL 2.4201 mL
    25 mM 0.0774 mL 0.3872 mL 0.7744 mL 1.9361 mL
    30 mM 0.0645 mL 0.3227 mL 0.6454 mL 1.6134 mL
    40 mM 0.0484 mL 0.2420 mL 0.4840 mL 1.2101 mL
    50 mM 0.0387 mL 0.1936 mL 0.3872 mL 0.9681 mL
    60 mM 0.0323 mL 0.1613 mL 0.3227 mL 0.8067 mL
    80 mM 0.0242 mL 0.1210 mL 0.2420 mL 0.6050 mL
    100 mM 0.0194 mL 0.0968 mL 0.1936 mL 0.4840 mL
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    Nitrocefin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Nitrocefin41906-86-9Beta-lactamaseAntibioticBacterialβ-lactamaseInhibitorinhibitorinhibit

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    Nombre del producto:
    Nitrocefin
    Cat. No.:
    HY-108913
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    SAFETY DATA SHEET (SDS)

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