2. Anti-infection
  3. Beta-lactamase Antibiotic Bacterial
  4. Nitrocefin

Nitrocefin is a highly activated, chromogenic cephalosporin derivative. Nitrocefin is a chromogenic β-lactamase substrate. Nitrocefin undergoes a distinctive color change from yellow to red as the amide bond in the β-lactam ring is hydrolyzed by β-lactamase. Nitrocefin is used in competitive inhibition studies in developmental work on β-lactamase-resistant antibiotics.

For research use only. We do not sell to patients.

CAS No. : 41906-86-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
1 mg In-stock
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Nitrocefin:

Nitrocefin Related Antibodies

Top Publications Citing Use of Products

2 Publications Citing Use of MCE Nitrocefin

Bio/Physico-chemical Assay

    Nitrocefin purchased from MedChemExpress. Usage Cited in: mBio. 2025 Apr 9;16(4):e0297524.  [Abstract]

    Cell lysates of WT, RP47 (∆sdpA), and RP56 (∆ampG) with and without ampicillin treatment were incubated with Nitrocefin solution (1 mg/mL) for 30 min. M2 buffer was used as a negative control. The appearance of red color product indicates a positive β-lactamase activity, while a consistent yellow color indicates the absence of activity.

    View All Beta-lactamase Isoform Specific Products:

    View All Isoforms
    Metallo-β-lactamase (MBL) Serine β-lactamase (SBL)

    View All Antibiotic Isoform Specific Products:

    View All Isoforms
    Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Nitrocefin is a highly activated, chromogenic cephalosporin derivative. Nitrocefin is a chromogenic β-lactamase substrate. Nitrocefin undergoes a distinctive color change from yellow to red as the amide bond in the β-lactam ring is hydrolyzed by β-lactamase. Nitrocefin is used in competitive inhibition studies in developmental work on β-lactamase-resistant antibiotics[1][2][3][4].

    IC50 & Target

    β-lactam

     

    In Vitro

    Nitrocefin is hydrolysised by β-Lactamase from Mycobacterium tuberculosis[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Nitrocefin Related Antibodies
    Molecular Weight

    516.50

    Formula

    C21H16N4O8S2
    CAS No.
    Appearance

    Solid

    Color

    Yellow to orange

    SMILES

    O=C(C(N12)=C(/C=C/C3=CC=C([N+]([O-])=O)C=C3[N+]([O-])=O)CS[C@]2([H])[C@H](NC(CC4=CC=CS4)=O)C1=O)O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (193.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9361 mL 9.6805 mL 19.3611 mL
    5 mM 0.3872 mL 1.9361 mL 3.8722 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 98.92%

    SDS (467 KB)

    COA (250 KB) HNMR (590 KB) RP-HPLC (240 KB) LCMS (94 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.9361 mL 9.6805 mL 19.3611 mL 48.4027 mL
    5 mM 0.3872 mL 1.9361 mL 3.8722 mL 9.6805 mL
    10 mM 0.1936 mL 0.9681 mL 1.9361 mL 4.8403 mL
    15 mM 0.1291 mL 0.6454 mL 1.2907 mL 3.2268 mL
    20 mM 0.0968 mL 0.4840 mL 0.9681 mL 2.4201 mL
    25 mM 0.0774 mL 0.3872 mL 0.7744 mL 1.9361 mL
    30 mM 0.0645 mL 0.3227 mL 0.6454 mL 1.6134 mL
    40 mM 0.0484 mL 0.2420 mL 0.4840 mL 1.2101 mL
    50 mM 0.0387 mL 0.1936 mL 0.3872 mL 0.9681 mL
    60 mM 0.0323 mL 0.1613 mL 0.3227 mL 0.8067 mL
    80 mM 0.0242 mL 0.1210 mL 0.2420 mL 0.6050 mL
    100 mM 0.0194 mL 0.0968 mL 0.1936 mL 0.4840 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Nitrocefin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Nitrocefin41906-86-9Beta-lactamaseAntibioticBacterialβ-lactamaseInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Nitrocefin
    Cat. No.:
    HY-108913
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (467 KB) Français - FR (467 KB) Deutsch - DE (467 KB) Norwegian - NO (467 KB) Español - ES (467 KB) Swedish - SV (467 KB) Italian - IT (467 KB) Korean - KR (467 KB) Portuguese - PT (467 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.