2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease Anti-infection
  3. Reactive Oxygen Species (ROS) HBV Endogenous Metabolite HIV Bacterial
  4. Isochlorogenic acid A

Isochlorogenic acid A  (Synonyms: 3,5-Dicaffeoylquinic acid; 3,5-CQA; イソクロロゲン酸A)

製品番号: HY-N0056 純度: 99.53%
COA 取扱説明書 Technical Support

Isochlorogenic acid A (3,5-Dicaffeoylquinic acid) is a natural phenolic acid with anti-mutagenicity, anti-HBV, anti-HIV, anti-oxidant, anti-bacterial, and anti-inflammatoryy activities.

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Isochlorogenic acid A

Isochlorogenic acid A 構造式

CAS 番号 : 2450-53-5

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 84 在庫あり
Solution
10 mM * 1 mL in DMSO USD 84 在庫あり
Solid
5 mg $74 在庫あり
10 mg $115 在庫あり
25 mg $240 在庫あり
50 mg $380 在庫あり
100 mg $580 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Based on 13 publication(s) in Google Scholar

Other Forms of Isochlorogenic acid A:

Isochlorogenic acid A 関連抗体

Top Publications Citing Use of Products

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  • 生物活性

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製品説明

Isochlorogenic acid A (3,5-Dicaffeoylquinic acid) is a natural phenolic acid with anti-mutagenicity, anti-HBV, anti-HIV, anti-oxidant, anti-bacterial, and anti-inflammatoryy activities[1].

Cellular Effect
Cell Line Type Value Description References
HeLa IC50
>500 μM
Compound: RLE30
Inhibition of HDAC in human HeLa cells using Boc-Lys(AC)-AMC as substrate after 24 to 48 hrs by spectrofluorometry
Inhibition of HDAC in human HeLa cells using Boc-Lys(AC)-AMC as substrate after 24 to 48 hrs by spectrofluorometry
[PMID: 26996372]
HT-22 EC50
>100 μM
Compound: 25
Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay
Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay
[PMID: 32991171]
MT2 ED50
1 μg/mL
Compound: 3,5-DCQA
Antiviral activity against HIV1 LAI in human MT2 cells assessed as virus-induced cytopathic effect after 72 hrs
Antiviral activity against HIV1 LAI in human MT2 cells assessed as virus-induced cytopathic effect after 72 hrs
[PMID: 11430019]
MT2 ED50
1.9 μM
Compound: 3,5-dicaffeoylquinic acid
Antiviral activity against HIV1 LAI in MT2 cells assessed as inhibition of virus-induced cytopathic effect
Antiviral activity against HIV1 LAI in MT2 cells assessed as inhibition of virus-induced cytopathic effect
[PMID: 18674899]
MT2 ED50
2 μM
Compound: 3,5-DCQA
Concentration that inhibits Human Immunodeficiency Virus Type 1 (HIV-1)-induced death of MT-2 cells
Concentration that inhibits Human Immunodeficiency Virus Type 1 (HIV-1)-induced death of MT-2 cells
[PMID: 9986720]
MT2 IC50
1.9 μM
Compound: 8
Inhibition of HIV1 integrase activity assessed as suppression of viral replication in human MT2 cells
Inhibition of HIV1 integrase activity assessed as suppression of viral replication in human MT2 cells
[PMID: 27676157]
体外実験

Isochlorogenic acid A inhibits the aminoacylation activity of LeuRS from Giardia lamblia (GlLeuRS), with an IC50 of 5.82 μg/mL[1].
Isochlorogenic acid A (5-100 μM, 48 h) increases melanin content in B16 cells, and increases TYR proteins activity[4].
Isochlorogenic acid A shows DPPH (IC50=4.26 µg/mL), ABTS radical scavenging activity[6].
Isochlorogenic acid A (0-250 μg/mL, 24 h) inhibits nitric oxide production in LPS-stimulated RAW 264.7 cells[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Isochlorogenic acid A 関連抗体

Western Blot Analysis[4]

Cell Line: B16 cells
Concentration: 48 h
Incubation Time: 5, 50, and 100 μM
Result: Increased tyrosinase (TYR), TRP1, TRP2, p-MITF, and total MITF protein expressions.
Induced the phosphorylation of Akt at Thr308.
体内実験

Isochlorogenic acid A (5 and 10 mg/kg, p.o., once a day for 3 weeks) ameliorates cognitive impairment induced by TMT in ICR male mice[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: TMT-induced ICR male mice[5]
Dosage: 5 and 10 mg/kg
Administration: p.o., once a day for 3 weeks
Result: Improved spatial memory and learning ability of mice in MWM test.
Reduced the TMT-induced increased AChE activity.
Reduced the MDA content compared to the TMT group.
分子量

516.45

分子式

C25H24O12
CAS 番号
Appearance

Solid

Color

White to light yellow

SMILES

OC(C=C1/C=C/C(O[C@H]2[C@@H]([C@@H](C[C@](O)(C2)C(O)=O)OC(/C=C/C3=CC(O)=C(C=C3)O)=O)O)=O)=C(C=C1)O
Structure Classification
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : ≥ 50 mg/mL (96.81 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9363 mL 9.6815 mL 19.3630 mL
5 mM 0.3873 mL 1.9363 mL 3.8726 mL
10 mM 0.1936 mL 0.9681 mL 1.9363 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (4.84 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.53%

COA (247 KB) HNMR (153 KB) LCMS (99 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9363 mL 9.6815 mL 19.3630 mL 48.4074 mL
5 mM 0.3873 mL 1.9363 mL 3.8726 mL 9.6815 mL
10 mM 0.1936 mL 0.9681 mL 1.9363 mL 4.8407 mL
15 mM 0.1291 mL 0.6454 mL 1.2909 mL 3.2272 mL
20 mM 0.0968 mL 0.4841 mL 0.9681 mL 2.4204 mL
25 mM 0.0775 mL 0.3873 mL 0.7745 mL 1.9363 mL
30 mM 0.0645 mL 0.3227 mL 0.6454 mL 1.6136 mL
40 mM 0.0484 mL 0.2420 mL 0.4841 mL 1.2102 mL
50 mM 0.0387 mL 0.1936 mL 0.3873 mL 0.9681 mL
60 mM 0.0323 mL 0.1614 mL 0.3227 mL 0.8068 mL
80 mM 0.0242 mL 0.1210 mL 0.2420 mL 0.6051 mL
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Isochlorogenic acid A Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Isochlorogenic acid A2450-53-53,5-Dicaffeoylquinic acid 3,5-CQAReactive Oxygen Species (ROS)HBVEndogenous MetaboliteHIVBacterialHepatitis B virusHuman immunodeficiency virusanti-mutagenicityanti-HBVanti-HIVanti-oxidantanti-bacterialanti-inflammatoryyInhibitorinhibitorinhibit

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