AKT Kinase Inhibitor

Name: AKT Kinase Inhibitor
Brand: MedChemExpress
SKU: HY-10249A
Rating: 5.0 (16 reviews)

製品番号: HY-10249A 純度: 99.92%: Data Sheet SDS COA 取扱説明書
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Technical Support

AKT Kinase Inhibitor is an Akt kinase inhibitor with anti-tumor activity.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 842148-40-7

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 462	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 462	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	$420	在庫あり	Estimated Time of Arrival: December 31
10 mg	$720	在庫あり	Estimated Time of Arrival: December 31
25 mg	$1120	在庫あり	Estimated Time of Arrival: December 31
50 mg	$1500	在庫あり	Estimated Time of Arrival: December 31
100 mg	$2000	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 16 publication(s) in Google Scholar

Other Forms of AKT Kinase Inhibitor:

顧客検証

Cell Migration/Invasion Assay

RT-PCR

: AKT Kinase Inhibitor purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2024 May 18:107218. [Abstract]; Stimulation of B16-F10 cells with TAM-conditioned medium, pre-treated with AKT Kinase Inhibitor (5 μM) for 24 h. Western blot assessing AKT phosphorylation, AKT baseline expression, and STC1 expression.

: AKT Kinase Inhibitor purchased from MedChemExpress. Usage Cited in: Pharmacol Res. 2024 May 18:107218. [Abstract]; Stimulation of B16-F10 cells with TAM-conditioned medium, pre-treated with AKT kinase inhibitor (5 μM) for 24 h. CCL2 mRNA expression was detected by qPCR.

: AKT Kinase Inhibitor purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2024 Apr 29;20(7):2748-2762. [Abstract]; Western blotting analysis of Akt1, p-Akt1 (Ser473), lamin A/C, and p-lamin A/C (Ser22) in SW480 LNCs treated with or without AKT Kinase Inhibitor (Akti; 0.5 μM).

: AKT Kinase Inhibitor purchased from MedChemExpress. Usage Cited in: Cell Stem Cell. 2023 Apr 6;30(4):450-459.e9. [Abstract]; Immunofluorescence of LIN28A at the end of stage I under SI_BMP4/B27+AKTi (AKT Kinase Inhibitor: 1 μM) +VTP+J condition.

: AKT Kinase Inhibitor purchased from MedChemExpress. Usage Cited in: Small. 2023 Apr;19(16):e2207194. [Abstract]; AKT Kinase Inhibitor (10 μM). Comparison of migration restriction ability of SK-Hep-1, SNU-449, PANC-1, SW1990 and primary cancer-associated fibroblasts.

: AKT Kinase Inhibitor purchased from MedChemExpress. Usage Cited in: Mediators Inflamm. 2020 Jun 24;2020:3621261. [Abstract]; After treatment with HY-10249A, the expression of p-IκB-α and p-NF-κB p65 is reduced.

Akt アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Akt Akt1 Akt2 Akt3

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

AKT Kinase Inhibitor is an Akt kinase inhibitor with anti-tumor activity^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
BT-474	IC₅₀	1.13 μM Compound: 12c	Inhibition of AKT-mediated GSK3-beta phosphorylation in human BT474 cells Inhibition of AKT-mediated GSK3-beta phosphorylation in human BT474 cells	[PMID: 18800763]
Sf9	IC₅₀	0.02 μM Compound: 12c	Inhibition of human recombinant ROCK1 expressed in Sf9 cells Inhibition of human recombinant ROCK1 expressed in Sf9 cells	[PMID: 18800763]

体外実験

The effect of selective inhibition of Akt in proliferating cells expressing Trop-2 is studied. Akt inhibition, either by silencing or with specific drugs, induces a marked decrease in cell proliferation in cells expressing Trop-2, in a dose-dependent fashion^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

Trop-2 expressing tumors subcutaneously injected in animal models show a striking reduction of growth following treatment with specific drugs that inhibit Akt activity. Therefore Akt has a central functional role in mediating the Trop-2-dependent growth stimulus^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

357.37

分子式

C₁₆H₁₉N₇O₃

CAS 番号

842148-40-7

Appearance

Solid

Color

White to light yellow

SMILES

OCC#CC1=NC=C(C2=C1N=C(N2CC)C3=NON=C3N)OCCCN

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 20 mg/mL (55.96 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7982 mL	13.9911 mL	27.9822 mL
5 mM	0.5596 mL	2.7982 mL	5.5964 mL
10 mM	0.2798 mL	1.3991 mL	2.7982 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.67 mg/mL (4.67 mM); Clear solution

This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.92%

Select Batch:

データシート (273 KB) SDS (254 KB)

COA (250 KB) HNMR (266 KB) RP-HPLC (256 KB) LCMS (94 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Saverio Alberti, et al. Use of trop-2 as predictive marker of response to anti-tumor therapy based on inhibitors of cd9, akt and molecules of the tetraspanin signalling network. WO2013171777A2.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7982 mL	13.9911 mL	27.9822 mL	69.9555 mL
	5 mM	0.5596 mL	2.7982 mL	5.5964 mL	13.9911 mL
	10 mM	0.2798 mL	1.3991 mL	2.7982 mL	6.9956 mL
	15 mM	0.1865 mL	0.9327 mL	1.8655 mL	4.6637 mL
	20 mM	0.1399 mL	0.6996 mL	1.3991 mL	3.4978 mL
	25 mM	0.1119 mL	0.5596 mL	1.1193 mL	2.7982 mL
	30 mM	0.0933 mL	0.4664 mL	0.9327 mL	2.3319 mL
	40 mM	0.0700 mL	0.3498 mL	0.6996 mL	1.7489 mL
	50 mM	0.0560 mL	0.2798 mL	0.5596 mL	1.3991 mL