アメントフラボン (Synonyms: Amentoflavone; Didemethyl-ginkgetin)

Name: Amentoflavone
Brand: MedChemExpress
SKU: HY-N0662
Rating: 5.0 (18 reviews)

製品番号: HY-N0662 純度: 99.66%: Data Sheet SDS COA 取扱説明書
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Amentoflavone (Didemethyl-ginkgetin) is a potent and orally active GABA(A) negative modulator. Amentoflavone also shows anti-inflammatory, antioxidative, anti-viral, anti-tumor, anti-radiation, anti-fungal, antibacterial activity. Amentoflavone induces apoptosis and cell cycle arrest at sub-G1 phase.

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CAS 番号 : 1617-53-4

容量	価格（税別）	在庫状況	数量

>無料サンプル (0.1 - 0.2 mg)		今すぐ申し込む
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 71	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 71	在庫あり	Estimated Time of Arrival: December 31
Solid
5 mg	$60	在庫あり	Estimated Time of Arrival: December 31
10 mg	$95	在庫あり	Estimated Time of Arrival: December 31
25 mg	$165	在庫あり	Estimated Time of Arrival: December 31
50 mg	$290	在庫あり	Estimated Time of Arrival: December 31
100 mg	$470	在庫あり	Estimated Time of Arrival: December 31
200 mg		お問い合わせ
500 mg		お問い合わせ

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カスタマーレビュー

Based on 18 publication(s) in Google Scholar

Other Forms of Amentoflavone:

Amentoflavone (Standard) お問い合わせ

顧客検証

Cell Imaging/Staining

Histological Imaging/Staining

Cell Proliferation/Viability Assay

: Amentoflavone purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2023 Aug:117:154922.; Mice were subjected to a 2-week intraperitoneal injection of saline (vehicle) or DOX with or without the combined administration of Amentoflavone (AMF) (20 mg/kg) and/or nigericin, Representative western blot.

: Amentoflavone purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2023 Aug:117:154922.; Mice were subjected to a 2-week intraperitoneal injection of saline (vehicle) or DOX with or without the combined administration of Amentoflavone (AMF) (20 mg/kg) and/or nigericin, Representative images of TUNEL staining.

: Amentoflavone purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2023 Aug:117:154922.; Mice were subjected to a 2-week intraperitoneal injection of saline (vehicle) or DOX with/without combined administration of Amentoflavone (AMF) (20 mg/kg), Representative images of the hematoxylineosin (HE) staining .

: Amentoflavone purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2023 Aug:117:154922.; Primary cardiomyocytes were subjected to saline (vehicle) or DOX (1 µM, 24 h) with/without combined incubation of Amentoflavone (AMF, 20 µM,24 h) and/or ABZI (1 µM, 3 h). Viability of cardiomyocytes was measured using the CCK-8 assay.

: Amentoflavone purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2023 Aug:117:154922.; Primary cardiomyocytes were subjected to saline (vehicle) or DOX (1 µM, 24 h) with/without combined incubation of Amentoflavone (AMF, 20 µM,24 h) and/or ABZI (1 µM, 3 h). Representative immunofluorescence staining of GSDMD-N.

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製品説明

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	8.36 3 Compound: 10	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 29921475]
A549	IC₅₀	1.6 6 Compound: 1	Cytotoxicity against A549 cells Cytotoxicity against A549 cells	[PMID: 17027271]
A549	IC₅₀	8.36 3 Compound: 10	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 29921475]
Calu-1	IC₅₀	> 100 3 Compound: 6	Cytotoxicity against human Calu1 cells after 3 days Cytotoxicity against human Calu1 cells after 3 days	[PMID: 10843573]
Fibroblast	IC₅₀	1.8 3 Compound: 4	Inhibition of UVB irradiation-induced MMP1 production in human dermal fibroblasts after 48 hrs Inhibition of UVB irradiation-induced MMP1 production in human dermal fibroblasts after 48 hrs	[PMID: 18029185]
HCT-15	IC₅₀	> 50 3 Compound: 8	Cytotoxicity against human HCT15 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HCT15 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
HeLa	IC₅₀	> 100 3 Compound: 6	Cytotoxicity against human HeLa cells after 3 days Cytotoxicity against human HeLa cells after 3 days	[PMID: 10843573]
Calu-1	IC₅₀	>100 3 Compound: 6	Cytotoxicity against human Calu1 cells after 3 days Cytotoxicity against human Calu1 cells after 3 days	[PMID: 10843573]
HeLa	IC₅₀	46.4 3 Compound: amentoflavone	Cytotoxicity against human HeLa cells assessed as growth inhibition by sulforhodamine B assay Cytotoxicity against human HeLa cells assessed as growth inhibition by sulforhodamine B assay	[PMID: 26722868]
HeLa	IC₅₀	5.3 3 Compound: 7	Antiproliferative activity against human HeLa cells after 48 hrs by CCK8 assay Antiproliferative activity against human HeLa cells after 48 hrs by CCK8 assay	[PMID: 29475587]
HepG2	IC₅₀	> 50 3 Compound: 8	Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
Fibroblast	IC₅₀	1.8 3 Compound: 4	Inhibition of UVB irradiation-induced MMP1 production in human dermal fibroblasts after 48 hrs Inhibition of UVB irradiation-induced MMP1 production in human dermal fibroblasts after 48 hrs	[PMID: 18029185]
HCT-15	IC₅₀	>50 3 Compound: 8	Cytotoxicity against human HCT15 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HCT15 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
HepG2	IC₅₀	9.13 3 Compound: 10	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 29921475]
HL-60	IC₅₀	> 50 3 Compound: 8	Cytotoxicity against human HL60 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HL60 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
A549	IC₅₀	1.6 6 Compound: 1	Cytotoxicity against A549 cells Cytotoxicity against A549 cells	[PMID: 17027271]
HL-60	IC₅₀	51.2 3 Compound: 7	Antiproliferative activity against human HL60 cells after 48 hrs by CCK8 assay Antiproliferative activity against human HL60 cells after 48 hrs by CCK8 assay	[PMID: 29475587]
A549	IC₅₀	8.36 3 Compound: 10	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 29921475]
Huh-7	CC₅₀	> 50 3 Compound: 12	Cytotoxicity against human Huh7.5.1 cells by MTT assay Cytotoxicity against human Huh7.5.1 cells by MTT assay	[PMID: 22445328]
B16	IC₅₀	1.6 6 Compound: 1	Cytotoxicity against B16 cells Cytotoxicity against B16 cells	[PMID: 17027271]
HeLa	IC₅₀	5.3 3 Compound: 7	Antiproliferative activity against human HeLa cells after 48 hrs by CCK8 assay Antiproliferative activity against human HeLa cells after 48 hrs by CCK8 assay	[PMID: 29475587]
Jurkat	IC₅₀	> 50 3 Compound: 8	Cytotoxicity against human Jurkat cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human Jurkat cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
BGC-823	IC₅₀	1.6 6 Compound: 1	Cytotoxicity against BGC823 cells Cytotoxicity against BGC823 cells	[PMID: 17027271]
HeLa	IC₅₀	>10 3 Compound: 66	Antiproliferative activity against human HeLa cells assessed as reduction in cell viability Antiproliferative activity against human HeLa cells assessed as reduction in cell viability	[PMID: 30830783]
K562	IC₅₀	> 100 3 Compound: 6	Cytotoxicity against human K562 cells after 3 days Cytotoxicity against human K562 cells after 3 days	[PMID: 10843573]
Bel-7402	IC₅₀	1.6 6 Compound: 1	Cytotoxicity against BEL-7402 cells Cytotoxicity against BEL-7402 cells	[PMID: 17027271]
MCF7	IC₅₀	75.4 3 Compound: 4	Cytotoxicity against human MCF7 cells measured after 24 hrs by MTT assay Cytotoxicity against human MCF7 cells measured after 24 hrs by MTT assay	[PMID: 34875389]
HeLa	IC₅₀	>100 3 Compound: 6	Cytotoxicity against human HeLa cells after 3 days Cytotoxicity against human HeLa cells after 3 days	[PMID: 10843573]
HeLa	IC₅₀	46.4 3 Compound: amentoflavone	Cytotoxicity against human HeLa cells assessed as growth inhibition by sulforhodamine B assay Cytotoxicity against human HeLa cells assessed as growth inhibition by sulforhodamine B assay	[PMID: 26722868]
MGC-803	IC₅₀	9.86 3 Compound: 10	Cytotoxicity against human MGC803 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human MGC803 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 29921475]
Monocyte	IC₅₀	3 3 Compound: amentoflavone	Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs	[PMID: 8882428]
HepG2	IC₅₀	>50 3 Compound: 8	Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
Neutrophil	IC₅₀	0.75 3 Compound: 7	Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release pretreated for 5 mins followed by fMLP/CB stimulation using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as substrate Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release pretreated for 5 mins followed by fMLP/CB stimulation using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as substrate	[PMID: 29381070]
HepG2	IC₅₀	9.13 3 Compound: 10	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 29921475]
Calu-1	IC₅₀	> 100 3 Compound: 6	Cytotoxicity against human Calu1 cells after 3 days Cytotoxicity against human Calu1 cells after 3 days	[PMID: 10843573]
DU-145	IC₅₀	1.6 6 Compound: 1	Cytotoxicity against DU145 cells Cytotoxicity against DU145 cells	[PMID: 17027271]
Neutrophil	IC₅₀	1.01 3 Compound: 7	Anti-inflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation pretreated for 5 mins followed by cytochalasin B addition for 3 mins and subsequent fMLP stimulation for 10 mins by superoxide dismutase Anti-inflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation pretreated for 5 mins followed by cytochalasin B addition for 3 mins and subsequent fMLP stimulation for 10 mins by superoxide dismutase	[PMID: 29381070]
HL-60	IC₅₀	51.2 3 Compound: 7	Antiproliferative activity against human HL60 cells after 48 hrs by CCK8 assay Antiproliferative activity against human HL60 cells after 48 hrs by CCK8 assay	[PMID: 29475587]
PBMC	IC₅₀	35 3 Compound: 1	Cytotoxicity against uninfected PHA-stimulated human PBMC assessed as thymidine incorporation after 24 hrs by trypan blue exclusion method Cytotoxicity against uninfected PHA-stimulated human PBMC assessed as thymidine incorporation after 24 hrs by trypan blue exclusion method	[PMID: 9322359]
Fibroblast	IC₅₀	1.8 3 Compound: 4	Inhibition of UVB irradiation-induced MMP1 production in human dermal fibroblasts after 48 hrs Inhibition of UVB irradiation-induced MMP1 production in human dermal fibroblasts after 48 hrs	[PMID: 18029185]
HL-60	IC₅₀	>50 3 Compound: 8	Cytotoxicity against human HL60 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HL60 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
HCT-15	IC₅₀	> 50 3 Compound: 8	Cytotoxicity against human HCT15 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HCT15 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
Raji	IC₅₀	> 100 3 Compound: 6	Cytotoxicity against human Raji cells after 3 days Cytotoxicity against human Raji cells after 3 days	[PMID: 10843573]
SW480	IC₅₀	111 3 Compound: 9	Cytotoxicity against human SW480 cells after 72 hrs by MTT assay Cytotoxicity against human SW480 cells after 72 hrs by MTT assay	[PMID: 15787435]
Huh-7	CC₅₀	>50 3 Compound: 12	Cytotoxicity against human Huh7.5.1 cells by MTT assay Cytotoxicity against human Huh7.5.1 cells by MTT assay	[PMID: 22445328]
HEK293	IC₅₀	23.8 3 Compound: Amentoflavone	Inhibition of sPLA2-2A in HEK293 cell Inhibition of sPLA2-2A in HEK293 cell	[PMID: 16504502]
Jurkat	IC₅₀	>50 3 Compound: 8	Cytotoxicity against human Jurkat cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human Jurkat cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
HEK293	IC₅₀	41.8 3 Compound: amentoflavone	Inhibition of human cloned sPLA2-5 expressed in HEK293 cells Inhibition of human cloned sPLA2-5 expressed in HEK293 cells	[PMID: 17826099]
T-24	IC₅₀	10.67 3 Compound: 10	Cytotoxicity against human T24 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human T24 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 29921475]
HEK293	IC₅₀	> 100 3 Compound: amentoflavone	Inhibition of human cloned cPLA2 expressed in HEK293 cells Inhibition of human cloned cPLA2 expressed in HEK293 cells	[PMID: 17826099]
K562	IC₅₀	>100 3 Compound: 6	Cytotoxicity against human K562 cells after 3 days Cytotoxicity against human K562 cells after 3 days	[PMID: 10843573]
THP-1	IC₅₀	> 50 3 Compound: 8	Cytotoxicity against human THP1 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human THP1 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
U-937	IC₅₀	> 50 3 Compound: 8	Cytotoxicity against human U937 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human U937 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
MCF7	IC₅₀	75.4 3 Compound: 4	Cytotoxicity against human MCF7 cells measured after 24 hrs by MTT assay Cytotoxicity against human MCF7 cells measured after 24 hrs by MTT assay	[PMID: 34875389]
HEK293	IC₅₀	> 100 3 Compound: amentoflavone	Inhibition of human cloned sPLA2-10 expressed in HEK293 cells Inhibition of human cloned sPLA2-10 expressed in HEK293 cells	[PMID: 17826099]
HEK293	IC₅₀	> 100 3 Compound: amentoflavone	Inhibition of rat cloned sPLA2-1B expressed in HEK293 cells Inhibition of rat cloned sPLA2-1B expressed in HEK293 cells	[PMID: 17826099]
MCF7	IC₅₀	1.6 6 Compound: 1	Cytotoxicity against MCF7 cells Cytotoxicity against MCF7 cells	[PMID: 17027271]
Vero	IC₅₀	> 100 3 Compound: 6	Cytotoxicity against african green monkey Vero cells after 3 days Cytotoxicity against african green monkey Vero cells after 3 days	[PMID: 10843573]
HL-60	IC₅₀	51.2 3 Compound: 7	Antiproliferative activity against human HL60 cells after 48 hrs by CCK8 assay Antiproliferative activity against human HL60 cells after 48 hrs by CCK8 assay	[PMID: 29475587]
WISH	IC₅₀	> 100 3 Compound: 6	Cytotoxicity against human Wish cells after 3 days Cytotoxicity against human Wish cells after 3 days	[PMID: 10843573]
HL-60	IC₅₀	> 50 3 Compound: 8	Cytotoxicity against human HL60 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HL60 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
MGC-803	IC₅₀	9.86 3 Compound: 10	Cytotoxicity against human MGC803 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human MGC803 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 29921475]
HT-29	IC₅₀	1.6 6 Compound: 1	Cytotoxicity against HT29 cells Cytotoxicity against HT29 cells	[PMID: 17027271]
Monocyte	IC₅₀	3 × 10^-6 41 Compound: amentoflavone	Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs	[PMID: 8882428]
Neutrophil	IC₅₀	0.75 3 Compound: 7	Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release pretreated for 5 mins followed by fMLP/CB stimulation using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as substrate Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release pretreated for 5 mins followed by fMLP/CB stimulation using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as substrate	[PMID: 29381070]
HeLa	IC₅₀	46.4 3 Compound: amentoflavone	Cytotoxicity against human HeLa cells assessed as growth inhibition by sulforhodamine B assay Cytotoxicity against human HeLa cells assessed as growth inhibition by sulforhodamine B assay	[PMID: 26722868]
Neutrophil	IC₅₀	1.01 3 Compound: 7	Anti-inflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation pretreated for 5 mins followed by cytochalasin B addition for 3 mins and subsequent fMLP stimulation for 10 mins by superoxide dismutase Anti-inflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation pretreated for 5 mins followed by cytochalasin B addition for 3 mins and subsequent fMLP stimulation for 10 mins by superoxide dismutase	[PMID: 29381070]
HeLa	IC₅₀	5.3 3 Compound: 7	Antiproliferative activity against human HeLa cells after 48 hrs by CCK8 assay Antiproliferative activity against human HeLa cells after 48 hrs by CCK8 assay	[PMID: 29475587]
HeLa	IC₅₀	> 10 3 Compound: 66	Antiproliferative activity against human HeLa cells assessed as reduction in cell viability Antiproliferative activity against human HeLa cells assessed as reduction in cell viability	[PMID: 30830783]
HeLa	IC₅₀	> 100 3 Compound: 6	Cytotoxicity against human HeLa cells after 3 days Cytotoxicity against human HeLa cells after 3 days	[PMID: 10843573]
PBMC	IC₅₀	35 3 Compound: 1	Cytotoxicity against uninfected PHA-stimulated human PBMC assessed as thymidine incorporation after 24 hrs by trypan blue exclusion method Cytotoxicity against uninfected PHA-stimulated human PBMC assessed as thymidine incorporation after 24 hrs by trypan blue exclusion method	[PMID: 9322359]
HepG2	IC₅₀	9.13 3 Compound: 10	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 29921475]
HepG2	IC₅₀	> 50 3 Compound: 8	Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
Raji	IC₅₀	>100 3 Compound: 6	Cytotoxicity against human Raji cells after 3 days Cytotoxicity against human Raji cells after 3 days	[PMID: 10843573]
Huh-7	CC₅₀	> 50 3 Compound: 12	Cytotoxicity against human Huh7.5.1 cells by MTT assay Cytotoxicity against human Huh7.5.1 cells by MTT assay	[PMID: 22445328]
Jurkat	IC₅₀	> 50 3 Compound: 8	Cytotoxicity against human Jurkat cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human Jurkat cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
SW480	IC₅₀	111 3 Compound: 9	Cytotoxicity against human SW480 cells after 72 hrs by MTT assay Cytotoxicity against human SW480 cells after 72 hrs by MTT assay	[PMID: 15787435]
K562	IC₅₀	> 100 3 Compound: 6	Cytotoxicity against human K562 cells after 3 days Cytotoxicity against human K562 cells after 3 days	[PMID: 10843573]
T-24	IC₅₀	10.67 3 Compound: 10	Cytotoxicity against human T24 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human T24 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 29921475]
THP-1	IC₅₀	>50 3 Compound: 8	Cytotoxicity against human THP1 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human THP1 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
MCF7	IC₅₀	1.6 6 Compound: 1	Cytotoxicity against MCF7 cells Cytotoxicity against MCF7 cells	[PMID: 17027271]
U-937	IC₅₀	>50 3 Compound: 8	Cytotoxicity against human U937 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human U937 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
MCF7	IC₅₀	75.4 3 Compound: 4	Cytotoxicity against human MCF7 cells measured after 24 hrs by MTT assay Cytotoxicity against human MCF7 cells measured after 24 hrs by MTT assay	[PMID: 34875389]
Vero	IC₅₀	>100 3 Compound: 6	Cytotoxicity against african green monkey Vero cells after 3 days Cytotoxicity against african green monkey Vero cells after 3 days	[PMID: 10843573]
MDA-MB-231	IC₅₀	1.6 6 Compound: 1	Cytotoxicity against MDA-MB-231 cells Cytotoxicity against MDA-MB-231 cells	[PMID: 17027271]
WISH	IC₅₀	>100 3 Compound: 6	Cytotoxicity against human Wish cells after 3 days Cytotoxicity against human Wish cells after 3 days	[PMID: 10843573]
MGC-803	IC₅₀	9.86 3 Compound: 10	Cytotoxicity against human MGC803 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human MGC803 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 29921475]
Monocyte	IC₅₀	3 x 10^-6 41 Compound: amentoflavone	Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs	[PMID: 8882428]
NIH3T3	IC₅₀	9.2 3 Compound: Amentoflavone	Inhibition of PDGF-induced phosphotidyl inositol turnover in PLCgamma1 expressing mouse NIH3T3 cells Inhibition of PDGF-induced phosphotidyl inositol turnover in PLCgamma1 expressing mouse NIH3T3 cells	[PMID: 10869194]
Neutrophil	IC₅₀	0.75 3 Compound: 7	Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release pretreated for 5 mins followed by fMLP/CB stimulation using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as substrate Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release pretreated for 5 mins followed by fMLP/CB stimulation using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as substrate	[PMID: 29381070]
Neutrophil	IC₅₀	1.01 3 Compound: 7	Anti-inflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation pretreated for 5 mins followed by cytochalasin B addition for 3 mins and subsequent fMLP stimulation for 10 mins by superoxide dismutase Anti-inflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation pretreated for 5 mins followed by cytochalasin B addition for 3 mins and subsequent fMLP stimulation for 10 mins by superoxide dismutase	[PMID: 29381070]
Oocyte	EC₅₀	3.6 3 Compound: Amentoflavone	Effective concentration against human recombinant GABA-A receptor alpha-1-beta-2-gamma-2 subunits expressed in Xenopus oocytes Effective concentration against human recombinant GABA-A receptor alpha-1-beta-2-gamma-2 subunits expressed in Xenopus oocytes	[PMID: 12824018]
PBMC	IC₅₀	35 3 Compound: 1	Cytotoxicity against uninfected PHA-stimulated human PBMC assessed as thymidine incorporation after 24 hrs by trypan blue exclusion method Cytotoxicity against uninfected PHA-stimulated human PBMC assessed as thymidine incorporation after 24 hrs by trypan blue exclusion method	[PMID: 9322359]
Raji	IC₅₀	> 100 3 Compound: 6	Cytotoxicity against human Raji cells after 3 days Cytotoxicity against human Raji cells after 3 days	[PMID: 10843573]
SGC-7901	IC₅₀	1.6 6 Compound: 1	Cytotoxicity against SGC7901 cells Cytotoxicity against SGC7901 cells	[PMID: 17027271]
SW480	IC₅₀	111 3 Compound: 9	Cytotoxicity against human SW480 cells after 72 hrs by MTT assay Cytotoxicity against human SW480 cells after 72 hrs by MTT assay	[PMID: 15787435]
T-24	IC₅₀	10.67 3 Compound: 10	Cytotoxicity against human T24 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human T24 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 29921475]
THP-1	IC₅₀	> 50 3 Compound: 8	Cytotoxicity against human THP1 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human THP1 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
U-937	IC₅₀	> 50 3 Compound: 8	Cytotoxicity against human U937 cells assessed as cell viability after 24 hrs by MTT assay Cytotoxicity against human U937 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 23206866]
Vero	IC₅₀	> 100 3 Compound: 6	Cytotoxicity against african green monkey Vero cells after 3 days Cytotoxicity against african green monkey Vero cells after 3 days	[PMID: 10843573]
WISH	IC₅₀	> 100 3 Compound: 6	Cytotoxicity against human Wish cells after 3 days Cytotoxicity against human Wish cells after 3 days	[PMID: 10843573]

体外実験

Amentoflavone (1-60 μM) inhibits the production of nitric oxide in a concentration-dependent manner in RAW 264.7 cells^[2].
Amentoflavone (50-200 μM) inhibits the viability of U-87 MG cells with IC₅₀ value of 100 μM at 48 h^[3].
Amentoflavone (0, 50, 100 μM; 48 h) induces apoptosis and cell cycle arrest at sub-G1 phase^[3].
Amentoflavone (0, 50, 100 μM; 48 h) inhibits NF- B activation and decreases the expression of MCL1 and C-FLIP protein in U-87 MG cells^[3].
Amentoflavone (0-32 μg/ml) shows antibacterial activity with MICs of 8, 4, 32, 8, 16, 8 μg/ml for E. faecium ATCC 19434, S. aureus ATCC 25923, S. mutans ATCC 3065, E. coli O-157 ATCC 25922, E. coli ATCC 43895, P. aeruginosa ATCC 27853, respectively^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	U-87 MG cells
Concentration:	0, 50, 75, 100, 200 µM
Incubation Time:	48 h
Result:	Significantly inhibited the viability of U-87 MG cells by 23-71% with an IC₅₀ value of 100 µM at 48 h.

Apoptosis Analysis^[3]

Cell Line:	U-87 MG cells
Concentration:	0, 50, 100 µM
Incubation Time:	48 h
Result:	Significantly induced the accumulation of cells in the sub-G1 population and increased the level of active caspase-3 by 14-52% and 24-42%, respectively, and significantly triggered the loss of Ψm and the expression of active caspase-8 by 23-53% and 25-50%, respectively.

Western Blot Analysis^[3]

Cell Line:	U-87 MG cells
Concentration:	0, 50, 100 µM
Incubation Time:	48 h
Result:	Significantly reduced NF-ĸB activation in a dose-dependent manner by 25-87% and reduced protein expression of MCL1 and C-FLIP by 50-80% and 38-57%, respectively.

体内実験

Amentoflavone (25 mg/kg; p.o.; once a day for 3 consecutive days) shows neuroprotective role in epilepsy via anti-inflammatory effects in mouse^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	5-6 weeks, 28-32 g, kunming mice^[1]
Dosage:	25 mg/kg
Administration:	P.o.; once a day for 3 consecutive days
Result:	Inhibited activation and nuclear translocation of NF-κB subunits p65, decreased IL-6 and IL-1β production and significantly decreased NO and prostaglandin E2 production.

分子量

538.46

分子式

C₃₀H₁₈O₁₀

CAS 番号

1617-53-4

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C=C(C2=CC=C(O)C=C2)OC3=C(C4=CC(C5=CC(C6=C(O)C=C(O)C=C6O5)=O)=CC=C4O)C(O)=CC(O)=C13

Structure Classification

Initial Source

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 100 mg/mL (185.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.8571 mL	9.2857 mL	18.5715 mL
5 mM	0.3714 mL	1.8571 mL	3.7143 mL
10 mM	0.1857 mL	0.9286 mL	1.8571 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.64 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (4.64 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.66%

Select Batch:

データシート (288 KB) SDS (393 KB)

COA (260 KB) HNMR (299 KB) LCMS (94 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Zhang Z, et al. Amentoflavone protects hippocampal neurons: anti-inflammatory, antioxidative, and antiapoptotic effects. Neural Regen Res. 2015 Jul;10(7):1125-33. [Content Brief]

[2]. Woo ER, et al. Amentoflavone inhibits the induction of nitric oxide synthase by inhibiting NF-kappaB activation in macrophages. Pharmacol Res. 2005 Jun;51(6):539-46. [Content Brief]

[3]. Yen TH, et al. Amentoflavone Induces Apoptosis and Inhibits NF-ĸB-modulated Anti-apoptotic Signaling in Glioblastoma Cells. In Vivo. 2018 Mar-Apr;32(2):279-285. [Content Brief]

[4]. Hwang JH, et al. Antibacterial effect of amentoflavone and its synergistic effect with antibiotics. J Microbiol Biotechnol. 2013;23(7):953-8. [Content Brief]

[5]. Hanrahan JR, et al. Semisynthetic preparation of amentoflavone: A negative modulator at GABA(A) receptors. Bioorg Med Chem Lett. 2003 Jul 21;13(14):2281-4. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.8571 mL	9.2857 mL	18.5715 mL	46.4287 mL
	5 mM	0.3714 mL	1.8571 mL	3.7143 mL	9.2857 mL
	10 mM	0.1857 mL	0.9286 mL	1.8571 mL	4.6429 mL
	15 mM	0.1238 mL	0.6190 mL	1.2381 mL	3.0952 mL
	20 mM	0.0929 mL	0.4643 mL	0.9286 mL	2.3214 mL
	25 mM	0.0743 mL	0.3714 mL	0.7429 mL	1.8571 mL
	30 mM	0.0619 mL	0.3095 mL	0.6190 mL	1.5476 mL
	40 mM	0.0464 mL	0.2321 mL	0.4643 mL	1.1607 mL
	50 mM	0.0371 mL	0.1857 mL	0.3714 mL	0.9286 mL
	60 mM	0.0310 mL	0.1548 mL	0.3095 mL	0.7738 mL
	80 mM	0.0232 mL	0.1161 mL	0.2321 mL	0.5804 mL
	100 mM	0.0186 mL	0.0929 mL	0.1857 mL	0.4643 mL

Amentoflavone Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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アメントフラボン (Synonyms: Amentoflavone; Didemethyl-ginkgetin)

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Other Forms of Amentoflavone:

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Molarity Calculator

Dilution Calculator

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アメントフラボン (Synonyms: Amentoflavone; Didemethyl-ginkgetin)

カスタマーレビュー

Other Forms of Amentoflavone:

アメントフラボン 関連抗体

顧客検証

おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

生物活性

純度とドキュメンテーション

参考文献

カスタマーレビュー

アメントフラボン 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Amentoflavone Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

最近チェックした製品:

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アメントフラボン関連抗体

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