D-Erythro-dihydrosphingosine (Synonyms: Sphinganine (d18:0))

Name: D-Erythro-dihydrosphingosine
Brand: MedChemExpress
SKU: HY-W019838
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Cat. No.: HY-W019838 Pureza: 98.0%: Ficha de datos
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D-Erythro-dihydrosphingosin directly inhibits cytosolic phospholipase A₂α (cPLA₂α) activity.

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D-Erythro-dihydrosphingosine Estructura química

No. CAS : 764-22-7

Tamaño	Stock	Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	Obtener un presupuesto
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Revisión del cliente

Based on 1 publication(s) in Google Scholar

Other Forms of D-Erythro-dihydrosphingosine:

D-Erythro-dihydrosphingosine-d₇ In-stock
D-Erythro-dihydrosphingosine (Standard) Obtener un presupuesto

1 Publications Citing Use of MCE D-Erythro-dihydrosphingosine

•Cell Host Microbe. 2026 Mar 11;34(3):460-478.e9. [Abstract]

Recomendaciones destacadas

•Related Screening Libraries:

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Ver todos los productos específicos de isoformas Endogenous Metabolite:

Ver todas las isoformas

Human Endogenous Metabolite Microbial Metabolite

Ver todos los productos específicos de isoformas Phospholipase:

Ver todas las isoformas

Phospholipase A PLD Phospholipase C

Actividad biológica
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Referencias
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Descripciòn

D-Erythro-dihydrosphingosin directly inhibits cytosolic phospholipase A₂α (cPLA₂α) activity.

IC₅₀ & Target

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
FL5.12	IC₅₀	3.5 μM Compound: 7	Cytotoxicity against mouse FL5.12A cells after 48 hrs by DAPI staining-based flow cytometric analysis Cytotoxicity against mouse FL5.12A cells after 48 hrs by DAPI staining-based flow cytometric analysis	[PMID: 27475534]
HBL-100	GI₅₀	25 μM Compound: 10d	Antiproliferative activity against human HBL100 cells after 48 hrs incubation by SRB assay Antiproliferative activity against human HBL100 cells after 48 hrs incubation by SRB assay	[PMID: 25899335]
HeLa	GI₅₀	15 μM Compound: 10d	Antiproliferative activity against human HeLa cells after 48 hrs incubation by SRB assay Antiproliferative activity against human HeLa cells after 48 hrs incubation by SRB assay	[PMID: 25899335]
Jurkat	IC₅₀	3.53 μM Compound: Dihydrosphingosine	Inhibition of wild type human Jurkat cells after 18 hrs by MTT assay Inhibition of wild type human Jurkat cells after 18 hrs by MTT assay	[PMID: 17400555]
Jurkat	IC₅₀	3.68 μM Compound: Dihydrosphingosine	Inhibition of 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR2 mutant cells proliferation after 18 hrs by MTT assay Inhibition of 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR2 mutant cells proliferation after 18 hrs by MTT assay	[PMID: 17400555]
Jurkat	IC₅₀	4.9 μM Compound: Dihydrosphingosine	Inhibition of 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR1 mutant cells proliferation after 18 hrs by MTT assay Inhibition of 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR1 mutant cells proliferation after 18 hrs by MTT assay	[PMID: 17400555]
Jurkat	IC₅₀	6.31 μM Compound: Dihydrosphingosine	Inhibition of human 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR3 mutant cells proliferation after 18 hrs by MTT assay Inhibition of human 2-amino-4-(4-heptyloxyphenyl)-2-methylbutanol-resistant human Jurkat SBR3 mutant cells proliferation after 18 hrs by MTT assay	[PMID: 17400555]
SW1573	GI₅₀	4.5 μM Compound: 10d	Antiproliferative activity against human SW1573 cells after 48 hrs incubation by SRB assay Antiproliferative activity against human SW1573 cells after 48 hrs incubation by SRB assay	[PMID: 25899335]
T47D	GI₅₀	22 μM Compound: 10d	Antiproliferative activity against human T47D cells after 48 hrs incubation by SRB assay Antiproliferative activity against human T47D cells after 48 hrs incubation by SRB assay	[PMID: 25899335]
WiDr	GI₅₀	19 μM Compound: 10d	Antiproliferative activity against human WiDr cells after 48 hrs incubation by SRB assay Antiproliferative activity against human WiDr cells after 48 hrs incubation by SRB assay	[PMID: 25899335]

In Vitro

D-Erythro-dihydrosphingosine (D-erythro-DHS) significantly inhibits mastoparan-, but not Na₃VO₄-, stimulated arachidonic acid release in PC12 cells. D-Erythro-dihydrosphingosine at 100 AM slightly inhibits [³H]Arachidonic acid release, and 5 μM Ionomycin alone stimulates the release significantly. In the presence of 5 μM Ionomycin, 100 μM C2-ceramide stimulates the release, but D-Erythro-dihydrosphingosine (100 μM) inhibits the release. D-Erythro-dihydrosphingosine inhibits arachidonic acid release in cells and/or cPLA₂α activity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

301.51

Fòrmula

C₁₈H₃₉NO₂

No. CAS

764-22-7

Appearance

Solid

Color

White to off-white

SMILES

CCCCCCCCCCCCCCC[C@@H](O)[C@@H](N)CO

Structure Classification

Others

Initial Source

Endogenous metabolite

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvente y solubilidad

In Vitro:

Ethanol : 50 mg/mL (165.83 mM; Need ultrasonic)

DMSO : 10 mg/mL (33.17 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3166 mL	16.5832 mL	33.1664 mL
5 mM	0.6633 mL	3.3166 mL	6.6333 mL
10 mM	0.3317 mL	1.6583 mL	3.3166 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.29 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 3

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (3.32 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 4

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1 mg/mL (3.32 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 5

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1 mg/mL (3.32 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: 99.55%

Select Batch:

Ficha de datos (267 KB) SDS (393 KB)

COA (239 KB) LCMS (288 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Nakamura H, et al. Inhibition of arachidonic acid release and cytosolic phospholipase A2 alpha activity by D-erythro-sphingosine. Eur J Pharmacol. 2004 Jan 19;484(1):9-17. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / Ethanol	1 mM	3.3166 mL	16.5832 mL	33.1664 mL	82.9160 mL
	5 mM	0.6633 mL	3.3166 mL	6.6333 mL	16.5832 mL
	10 mM	0.3317 mL	1.6583 mL	3.3166 mL	8.2916 mL
	15 mM	0.2211 mL	1.1055 mL	2.2111 mL	5.5277 mL
	20 mM	0.1658 mL	0.8292 mL	1.6583 mL	4.1458 mL
	25 mM	0.1327 mL	0.6633 mL	1.3267 mL	3.3166 mL
	30 mM	0.1106 mL	0.5528 mL	1.1055 mL	2.7639 mL
Ethanol	40 mM	0.0829 mL	0.4146 mL	0.8292 mL	2.0729 mL
	50 mM	0.0663 mL	0.3317 mL	0.6633 mL	1.6583 mL
	60 mM	0.0553 mL	0.2764 mL	0.5528 mL	1.3819 mL
	80 mM	0.0415 mL	0.2073 mL	0.4146 mL	1.0364 mL
	100 mM	0.0332 mL	0.1658 mL	0.3317 mL	0.8292 mL

D-Erythro-dihydrosphingosine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

D-Erythro-dihydrosphingosine764-22-7Sphinganine (d18:0)Endogenous MetabolitePhospholipaseInhibitorinhibitorinhibit

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D-Erythro-dihydrosphingosine (Synonyms: Sphinganine (d18:0))

Revisión del cliente

Other Forms of D-Erythro-dihydrosphingosine:

D-Erythro-dihydrosphingosine Related Antibodies

1 Publications Citing Use of MCE D-Erythro-dihydrosphingosine

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas Endogenous Metabolite:

Ver todos los productos específicos de isoformas Phospholipase:

Actividad biológica

Pureza y Documentación

Referencias

Revisión del cliente

D-Erythro-dihydrosphingosine Related Antibodies

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

D-Erythro-dihydrosphingosine Related Classifications

Keywords:

Productos vistos recientemente:

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