2. Anti-infection
  3. Bacterial Antibiotic
  4. G-418 disulfate

Geneticin sulfate  (Synonyms: G-418 (disulfate))

製品番号: HY-17561 純度: 99.85%
COA 取扱説明書 Technical Support

G-418 disulfate (Geneticin sulfate), is an aminoglycoside antibiotic, inhibits protein synthesis in eukaryotes and prokaryotes. G-418 disulfate is commonly used as a selective agent for eukaryotic cells.

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G-418 disulfate

Geneticin sulfate 構造式

CAS 番号 : 108321-42-2

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 USD 66 在庫あり
Solution
10 mM * 1 mL in Water USD 66 在庫あり
Solid
500 mg $60 在庫あり
1 g $95 在庫あり
5 g $350 在庫あり
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50 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Based on 61 publication(s) in Google Scholar

Other Forms of G-418 disulfate:

Geneticin sulfate 関連抗体

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顧客検証

Antibiotic アイソフォーム固有の製品をすべて表示:

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Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

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製品説明

G-418 disulfate (Geneticin sulfate), is an aminoglycoside antibiotic, inhibits protein synthesis in eukaryotes and prokaryotes. G-418 disulfate is commonly used as a selective agent for eukaryotic cells[1].

IC50 & Target

Aminoglycoside

 

体外実験

G418 sulfate, an aminoglycoside neomycin analogue, interferes with protein synthesis and has been used extensively for selection of mammalian cell lines that possess neomycin resistance (NR). The neomycin resistance (neo) gene is frequently used in eukaryotic vectors as a dominant selectable gene. G418 can be covalently bound to asialoorosomucoid (AsOR) to form a conjugate for hepatocyte-specific targeting and toxicity[2].
The human GD3 synthase cDNA was transfected into MDA-MB231 cells, and G-418 bulk selection was used to select cells stably overexpressing the GD3 synthase[2]. An aminoglycoside antibiotic, G418, has been shown to be an inhibitor of many pro- and eukaryotes at concentrations from 1-300 microgram/ml[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Geneticin sulfate 関連抗体
分子量

692.71

分子式

C20H44N4O18S2
CAS 番号
Appearance

Solid

Color

White to light yellow

SMILES

O=S(O)(O)=O.O[C@@H]([C@@H]1O[C@@]([C@@H]([C@@H](O)[C@@H]2O)N)([H])O[C@]2([H])[C@H](O)C)[C@H]([C@@H](C[C@@H]1N)N)O[C@](OC[C@](C)(O)[C@@H]3NC)([H])[C@@H]3O.O=S(O)(O)=O
Structure Classification
Initial Source

Micromonospora rhodorangea
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, stored under nitrogen, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)
溶剤 & 溶解度
体外: 

H2O : 100 mg/mL (144.36 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4436 mL 7.2180 mL 14.4361 mL
5 mM 0.2887 mL 1.4436 mL 2.8872 mL
10 mM 0.1444 mL 0.7218 mL 1.4436 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (72.18 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
純度とドキュメンテーション

純度: 99.94%

COA (265 KB) HNMR (322 KB) RP-HPLC (201 KB)

取扱説明書 (2659 KB)
参考文献
動物実験
[3]

To characterize the sensitivity of the trypanosome populations to G418 in vivo, bloodstream forms of T. brucei brucei GUTat 3.1 and T. brucei brucei GUTat 3.1/BBR3 are expanded separately in sublethally irradiated mice. Prior to the first peak of parasitemia, trypanosomes are collected, and aliquots containing 106 trypanosomes are inoculated intraperitoneally into mice. Twenty-four hours following infection, the mice are divided into groups and treated with G418 at a dose of 10, 20, 30, 40, 50, or 80 mg/kg of body weight (bw) by inoculating intraperitoneally 0.2 mL of the drug in sterile water. At 24 and 48 h following the first treatment, G418 is administered to animals in each group at the same dose as before, resulting in three treatments per mouse. Repeated drug treatments are necessary to ensure complete elimination of nontransfected GUTat 3.1 parasites from the mice. Mice are then monitored daily, for 33 days, for the presence of parasites by microscopic examination of wet-blood films. Animals found to be parasitemic are recorded and then removed from the experiment.

MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.4436 mL 7.2180 mL 14.4361 mL 36.0901 mL
5 mM 0.2887 mL 1.4436 mL 2.8872 mL 7.2180 mL
10 mM 0.1444 mL 0.7218 mL 1.4436 mL 3.6090 mL
15 mM 0.0962 mL 0.4812 mL 0.9624 mL 2.4060 mL
20 mM 0.0722 mL 0.3609 mL 0.7218 mL 1.8045 mL
25 mM 0.0577 mL 0.2887 mL 0.5774 mL 1.4436 mL
30 mM 0.0481 mL 0.2406 mL 0.4812 mL 1.2030 mL
40 mM 0.0361 mL 0.1805 mL 0.3609 mL 0.9023 mL
50 mM 0.0289 mL 0.1444 mL 0.2887 mL 0.7218 mL
60 mM 0.0241 mL 0.1203 mL 0.2406 mL 0.6015 mL
80 mM 0.0180 mL 0.0902 mL 0.1805 mL 0.4511 mL
100 mM 0.0144 mL 0.0722 mL 0.1444 mL 0.3609 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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G-418 disulfate Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

G-418 disulfate108321-42-2GeneticinAntibiotic G-418Antibiotic G418Antibiotic G 418BacterialAntibioticproteinsynthesiseukaryoticcellsInhibitorinhibitorinhibit

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