Genipin (Synonyms: (+)-Genipin)

Name: Genipin
Brand: MedChemExpress
SKU: HY-17389
Rating: 5.0 (22 reviews)

Cat. No.: HY-17389 Pureza: 99.80%: Ficha de datos
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Genipin ((+)-Genipin) is a natural crosslinking reagent derived from Gardenia jasminoides Ellis fruits. Genipin inhibits UCP2 (uncoupling protein 2) in cells. Genipin has a variety of bioactivities, including modulation on proteins, antitumor, anti-inflammation, immunosuppression, antithrombosis, and protection of hippocampal neurons. Genipin also can be used for type 2 diabetes research.

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No. CAS : 6902-77-8

Tamaño	Stock	Cantidad

Muestra gratis (0.1 - 0.2 mg)	Solicite ahora
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtener un presupuesto
500 mg	Obtener un presupuesto

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Revisión del cliente

Based on 22 publication(s) in Google Scholar

Other Forms of Genipin:

Genipin (Standard) In-stock
7-Deoxy-10-hydroxyloganetin Obtener un presupuesto

22 Publications Citing Use of MCE Genipin

In Vivo Efficacy Study

Flow Cytometry

Cell Imaging/Staining

Histological Imaging/Staining

: Genipin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jun 5;16(1):5215. [Abstract]; Genipin (50 mg/kg, p.o.)-pretreated mice exhibited significantly improved tear secretion and corneal lesions.

: Genipin purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Jan 23:80:103511. [Abstract]; UCP2 inhibitor Genipin (30 mg/kg, i.p.) significantly decreased expression levels of UCP2, LC3-Ⅱ/LC3-Ⅰ and PINK1 in the I/R myocardium by western blots.

: Genipin purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Jan 23:80:103511. [Abstract]; Genipin (30 mg/kg, i.p.) significantly decreased expression levels of UCP2, LC3-Ⅱ/LC3-Ⅰ and PINK1 in the I/R myocardium by confocal images.

: Genipin purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Jan 23:80:103511. [Abstract]; The histological features of myocardial I/R injury were significantly mitigated by NRG-1 postconditioning compared with the I/R procedure and Genipin (30 mg/kg, i.p.) treatment.

: Genipin purchased from MedChemExpress. Usage Cited in: Mol Med. 2025 Oct 9;31(1):310. [Abstract]; RA-derived CD4⁺ T cells were treated with Genipin (25 μM) on day 3 and ROS levels were measured after 24 h.

: Genipin purchased from MedChemExpress. Usage Cited in: Molecules. 2018 Mar 16;23(3). pii: E675. [Abstract]; Representative image of immunobloting of UCP2 in the cultured AC16 cells under various treatments.

: Genipin purchased from MedChemExpress. Usage Cited in: Epigenetics. 2018;13(3):310-317. [Abstract]; Reduced BDNF protein induced by prenatal stress is normalized by the treatment of Genipin.

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Actividad biológica
Protocolo
Pureza y Documentación
Referencias
Revisión del cliente

Descripciòn

Cellular Effect

Cell Line	Type	Value	Description	References
Huh-7	IC₅₀	20 μg/mL Compound: 9	Antiviral activity against HCV infected in human Huh7 cells assessed as inhibition of viral replication after 72 hrs by NS5A staining Antiviral activity against HCV infected in human Huh7 cells assessed as inhibition of viral replication after 72 hrs by NS5A staining	[PMID: 19904996]
RAW264.7	IC₅₀	11.14 μM Compound: 8	Inhibition of nitric oxide production in lipopolysaccharide-activated mouse RAW264.7 cells by Griess reaction based method Inhibition of nitric oxide production in lipopolysaccharide-activated mouse RAW264.7 cells by Griess reaction based method	[PMID: 23305920]

In Vitro

Genipin increases mitochondrial membrane potential, increased ATP levels, closed KATP channels, and stimulated insulin secretion in pancreatic islet cells. Genipin causes suppression of insulin signal transduction through hyperactivation of c-Jun N-terminal kinase (JNK) and subsequent serine phosphorylation of insulin receptor substrate-1 (IRS-1), thus impairing insulin-stimulated glucose uptake in 3T3-L1 adipocytes^[1].
Genipin activates IRS-1, PI3-K, and downstream signaling pathway and increases concentrations of calcium, resulting in glucose transporter 4 (GLUT4) translocation and glucose uptake increase in C2C12 myotubes^[1].
Cytochrome c content increases significantly in the cytosol of Genipin-treated FaO cells. Activation of caspase-3 and caspase-7 is ultimately responsible for Genipin-induced apoptotic process in hepatoma cells. ROS level notably increases in Hep3B cells treated with 200 μM Genipin^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ensayo clínico

Peso molecular

226.23

Fòrmula

C₁₁H₁₄O₅

No. CAS

6902-77-8

Appearance

Solid

Color

Off-white to light brown

SMILES

O=C(C1=CO[C@@H](O)[C@@]2([H])[C@]1([H])CC=C2CO)OC

Structure Classification

Initial Source

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

4°C, protect from light

*In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

Solvente y solubilidad

In Vitro:

DMSO : ≥ 100 mg/mL (442.03 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 4 mg/mL (17.68 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.4203 mL	22.1014 mL	44.2028 mL
5 mM	0.8841 mL	4.4203 mL	8.8406 mL
10 mM	0.4420 mL	2.2101 mL	4.4203 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (protect from light). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (11.05 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 9.09 mg/mL (40.18 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: 99.80%

Select Batch:

Ficha de datos (282 KB) SDS (480 KB)

COA (257 KB) HNMR (171 KB) RP-HPLC (246 KB) MS (72 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Kim BC, et al. Genipin-induced apoptosis in hepatoma cells is mediated by reactive oxygen species/c-Jun NH2-terminal kinase-dependent activation of mitochondrial pathway. Biochem Pharmacol. 2005 Nov 1;70(9):1398-407. [Content Brief]

[2]. Ma C, et al. Genipin stimulates glucose transport in C2C12 myotubes via IRS-1 and calcium- dependent mechanism. J Endocrinol. 2012 Dec 20. [Content Brief]

[3]. Kim BC, et al. Genipin-induced apoptosis in hepatoma cells is mediated by reactive oxygen species/c-Jun NH2-terminal kinase-dependent activation of mitochondrial pathway. Biochem Pharmacol. 2005 Nov 1;70(9):1398-407.

[4]. Ye D, et al. Genipin normalizes depression-like behavior induced by prenatal stress through inhibiting DNMT1. Epigenetics. 2018 Mar 9:1-22. [Content Brief]

Ensayo de quinasas
^[3]

Briefly, the peptide substrate N-acetyl-Asp-Glu-Val-Asp-ρ-nitroanilide (Ac-DEVD-ρNA) is added to the cell lysates in assay buffer (50 mM HEPES, pH 7.4, 100 mM NaCl, 0.1% CHAPS, 10 mM dithiothreitol, 1 mM EDTA, 10% glycerol) and incubated at 37°C. The cleavage of the substrate is monitored at 405 nm.

MCE no ha confirmado la precisión de estos métodos independientemente. Son solo para referencia.

Referencias

[1]. Kim BC, et al. Genipin-induced apoptosis in hepatoma cells is mediated by reactive oxygen species/c-Jun NH2-terminal kinase-dependent activation of mitochondrial pathway. Biochem Pharmacol. 2005 Nov 1;70(9):1398-407. [Content Brief]

[2]. Ma C, et al. Genipin stimulates glucose transport in C2C12 myotubes via IRS-1 and calcium- dependent mechanism. J Endocrinol. 2012 Dec 20. [Content Brief]

[3]. Kim BC, et al. Genipin-induced apoptosis in hepatoma cells is mediated by reactive oxygen species/c-Jun NH2-terminal kinase-dependent activation of mitochondrial pathway. Biochem Pharmacol. 2005 Nov 1;70(9):1398-407.

[4]. Ye D, et al. Genipin normalizes depression-like behavior induced by prenatal stress through inhibiting DNMT1. Epigenetics. 2018 Mar 9:1-22. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	4.4203 mL	22.1014 mL	44.2028 mL	110.5070 mL
	5 mM	0.8841 mL	4.4203 mL	8.8406 mL	22.1014 mL
	10 mM	0.4420 mL	2.2101 mL	4.4203 mL	11.0507 mL
	15 mM	0.2947 mL	1.4734 mL	2.9469 mL	7.3671 mL
DMSO	20 mM	0.2210 mL	1.1051 mL	2.2101 mL	5.5254 mL
	25 mM	0.1768 mL	0.8841 mL	1.7681 mL	4.4203 mL
	30 mM	0.1473 mL	0.7367 mL	1.4734 mL	3.6836 mL
	40 mM	0.1105 mL	0.5525 mL	1.1051 mL	2.7627 mL
	50 mM	0.0884 mL	0.4420 mL	0.8841 mL	2.2101 mL
	60 mM	0.0737 mL	0.3684 mL	0.7367 mL	1.8418 mL
	80 mM	0.0553 mL	0.2763 mL	0.5525 mL	1.3813 mL
	100 mM	0.0442 mL	0.2210 mL	0.4420 mL	1.1051 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Genipin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Genipin6902-77-8(+)-GenipinAutophagyUCP2crosslinkingreagenttype-2diabetesproteinsantitumoranti-inflammationimmunosuppressionantithrombosishippocampalDNMT1Inhibitorinhibitorinhibit

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Genipin (Synonyms: (+)-Genipin)

Revisión del cliente

Other Forms of Genipin:

Genipin Related Antibodies

22 Publications Citing Use of MCE Genipin

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Actividad biológica

Protocolo

Pureza y Documentación

Referencias

Revisión del cliente

Genipin Related Antibodies

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

Genipin Related Classifications

Keywords:

Productos vistos recientemente:

Revisión del cliente

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