Indole-3-carbinol (Synonyms: Indole-3-methanol; 3-インドールメタノール)

Name: Indole-3-carbinol
Brand: MedChemExpress
SKU: HY-N0170
Rating: 5.0 (16 reviews)

製品番号: HY-N0170 純度: 99.16%: Data Sheet SDS COA 取扱説明書
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Indole-3-carbinol (I3C) inhibits NF-κB activity and also is an Aryl hydrocarbon receptor (AhR) agonist, and an inhibitor of WWP1 (WW domain-containing ubiquitin E3 ligase 1).

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CAS 番号 : 700-06-1

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 33	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 33	在庫あり	Estimated Time of Arrival: December 31
Solid
1 g	$30	在庫あり	Estimated Time of Arrival: December 31
5 g	$60	在庫あり	Estimated Time of Arrival: December 31
10 g	$105	在庫あり	Estimated Time of Arrival: December 31
50 g		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 16 publication(s) in Google Scholar

Other Forms of Indole-3-carbinol:

Indole-3-carbinol (Standard) 在庫あり

顧客検証

IHC

RT-PCR

: Indole-3-carbinol purchased from MedChemExpress. Usage Cited in: Mol Biol Lett. 2025 May 3;30(1):56. [Abstract]; Indole-3-carbinol (I3C: 20/40 mg/kg). Immunohistochemical analysis of Ki67 expression in tumors.

: Indole-3-carbinol purchased from MedChemExpress. Usage Cited in: Mol Biol Lett. 2025 May 3;30(1):56. [Abstract]; Indole-3-carbinol (I3C: 20/40 mg/kg). Protein levels of USP13 and WWP1 in nude mouse tumors were detected by Western blot.

: Indole-3-carbinol purchased from MedChemExpress. Usage Cited in: NPJ Precis Oncol. 2024 Jul 16;8(1):144. [Abstract]; The combined use of indole-3-carbinol (I3C) and SHP099 significantly inhibited pAKT levels. Different CRC cell lines were treated with I3C (200 μM) or SHP099 (20 μM), respectively.

: Indole-3-carbinol purchased from MedChemExpress. Usage Cited in: Molecules. 2018 Oct 11;23(10). pii: E2600. [Abstract]; The cells are cultured with Indole-3-carbinol (I3C: 50 μM) or 3MC (1 μM) in the presence of IPRN (20 μM). The IPRN-induced inhibition of nucleocytoplasmic shuttling of AhR is suppressed by I3C and 3MC.

: Indole-3-carbinol purchased from MedChemExpress. Usage Cited in: Molecules. 2018 Oct 11;23(10). pii: E2600. [Abstract]; The cells are cultured with Indole-3-carbinol (I3C: 50 μM) or 3MC (1 μM) in the presence of IPRN (20 μM). The mRNA expression of CYP1A1 is increased after the cotreatment of I3C and 3MC.

NF-κB アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Human Endogenous Metabolite Microbial Metabolite

生物活性
プロトコル
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

Indole-3-carbinol (I3C) inhibits NF-κB activity and also is an Aryl hydrocarbon receptor (AhR) agonist, and an inhibitor of WWP1 (WW domain-containing ubiquitin E3 ligase 1).

IC₅₀ & Target^[1]^[2]

NF-κB

AhR

Human Endogenous Metabolite

Cellular Effect

Cell Line	Type	Value	Description	References
U-251	EC₅₀	390 3 Compound: 3; I3C	Cytotoxicity against human U251 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay Cytotoxicity against human U251 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay	[PMID: 28256372]
GL261	IC₅₀	100 3 Compound: indole-3-carbinol	Anti-proliferative activity against mouse GL261 cells assessed as cell viability incubated for 2 hrs by CCK8 assay Anti-proliferative activity against mouse GL261 cells assessed as cell viability incubated for 2 hrs by CCK8 assay	[PMID: 37861443]
U-251	EC₅₀	390 3 Compound: 3; I3C	Cytotoxicity against human U251 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay Cytotoxicity against human U251 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay	[PMID: 28256372]
U-87MG ATCC	EC₅₀	290 3 Compound: 3; I3C	Cytotoxicity against human U87 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay Cytotoxicity against human U87 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay	[PMID: 28256372]
U-87MG ATCC	EC₅₀	290 3 Compound: 3; I3C	Cytotoxicity against human U87 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay Cytotoxicity against human U87 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay	[PMID: 28256372]
U-87MG ATCC	IC₅₀	526 3 Compound: 1, I3C	Antiproliferative activity against human U87MG cells assessed as inhibition of cell growth after 48 hrs by MTS assay Antiproliferative activity against human U87MG cells assessed as inhibition of cell growth after 48 hrs by MTS assay	[PMID: 23419325]
U-87MG ATCC	IC₅₀	>100 3 Compound: indole-3-carbinol	Anti-proliferative activity against human U-87 MG cells assessed as cell viability incubated for 2 hrs by CCK8 assay Anti-proliferative activity against human U-87 MG cells assessed as cell viability incubated for 2 hrs by CCK8 assay	[PMID: 37861443]
GL261	IC₅₀	100 3 Compound: indole-3-carbinol	Anti-proliferative activity against mouse GL261 cells assessed as cell viability incubated for 2 hrs by CCK8 assay Anti-proliferative activity against mouse GL261 cells assessed as cell viability incubated for 2 hrs by CCK8 assay	[PMID: 37861443]
U-87MG ATCC	IC₅₀	526 3 Compound: 1, I3C	Antiproliferative activity against human U87MG cells assessed as inhibition of cell growth after 48 hrs by MTS assay Antiproliferative activity against human U87MG cells assessed as inhibition of cell growth after 48 hrs by MTS assay	[PMID: 23419325]
U-251	EC₅₀	390 3 Compound: 3; I3C	Cytotoxicity against human U251 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay Cytotoxicity against human U251 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay	[PMID: 28256372]
U-87MG ATCC	EC₅₀	290 3 Compound: 3; I3C	Cytotoxicity against human U87 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay Cytotoxicity against human U87 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay	[PMID: 28256372]
U-87MG ATCC	IC₅₀	526 3 Compound: 1, I3C	Antiproliferative activity against human U87MG cells assessed as inhibition of cell growth after 48 hrs by MTS assay Antiproliferative activity against human U87MG cells assessed as inhibition of cell growth after 48 hrs by MTS assay	[PMID: 23419325]
U-87MG ATCC	IC₅₀	> 100 3 Compound: indole-3-carbinol	Anti-proliferative activity against human U-87 MG cells assessed as cell viability incubated for 2 hrs by CCK8 assay Anti-proliferative activity against human U-87 MG cells assessed as cell viability incubated for 2 hrs by CCK8 assay	[PMID: 37861443]

体外実験

It is found that Indole-3-carbinol (I3C) inhibits the proliferation of THP-1 cells in a dose- and time dependent manner with minimal toxicity over normal monocytes. The AhR target genes (CYP1A1, IL1β) are overexpressed upon Indole-3-carbinol treatment (p<0.05 to p<0.001). The antiproliferative effects of Indole-3-carbinol are in association with programing cell death. Indole-3-carbinol downregulates BCL2 and upregulates FasR in THP-1 cells (p<0.05 to p<0.001). G1 cell cycle arrest is also observed using flow cytometry. G1-acting cell cycle genes (P21, P27 and P53) are overexpressed (p<0.05 to p<0.001), while CDK2 is downregulated upon Indole-3-carbinol treatment (p<0.01 to p<0.001)^[1].Indole-3-carbinol suppresses NF-κB activity and stimulates the p53 pathway in pre-B acute lymphoblastic leukemia cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

臨床実験

分子量

147.18

分子式

C₉H₉NO

CAS 番号

700-06-1

Appearance

Solid

Color

White to off-white

SMILES

OCC1=CNC2=C1C=CC=C2

Structure Classification

Initial Source

Endogenous metabolite

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶剤 & 溶解度

体外:

DMSO : 100 mg/mL (679.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.7944 mL	33.9720 mL	67.9440 mL
5 mM	1.3589 mL	6.7944 mL	13.5888 mL
10 mM	0.6794 mL	3.3972 mL	6.7944 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (16.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (16.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (16.99 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.16%

Select Batch:

データシート (279 KB) SDS (393 KB)

COA (251 KB) HNMR (234 KB) RP-HPLC (243 KB) MS (228 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Mohammadi S, et al. Indole-3-carbinol induces G1 cell cycle arrest and apoptosis through aryl hydrocarbon receptor in THP-1 monocytic cell line.J Recept Signal Transduct Res. 2017 Oct;37(5):506-514. [Content Brief]

[2]. Safa M, et al. Indole-3-carbinol suppresses NF-κB activity and stimulates the p53 pathway in pre-B acute lymphoblastic leukemia cells. Tumour Biol. 2015 May;36(5):3919-30. [Content Brief]

[3]. Lee YR, et al. Reactivation of PTEN tumor suppressor for cancer treatment through inhibition of a MYC-WWP1 inhibitory pathway. Science. 2019 May 17;364(6441). pii: eaau0159. [Content Brief]

細胞実験
^[1]

THP-1 cells are cultured in RPMI 1640 supplemented with 10% FBS, 100 U/mL penicillin, 100 mg/mL streptomycin and 2 mM Glutamax in a fully humidified atmosphere with 5% CO₂. Cells (2-5×10⁵ mL^-1) are seeded in six well plates followed by resuspension in complete growth media. THP-1 monocyte cells are then treated with 10 ng/mL phorbol 12-myristate 13-acetate as a tumor promoter to induce stable differentiation into attaching macrophage-like cells and overexpression of AhR. The cells are then treated with varying concentrations of Indole-3-carbinol (1, 10 and 100 μM, and 1 mM). THP-1 cells and enriching normal monocytes are seeded at 5×10⁴ cells/well in 24-well plate with different concentrations of Indole-3-carbinol and observed for 24 and 48 h followed by MTT assay. The cells are incubated in triplicates in a final volume of 200 mL of Phenol Red free RPMI 1640 for 20 h . An aliquot of 20 mL of MTT solution (5 mg/mL) is added to each well and incubated for 4 h. Formazan crystals are formed. An amount of 300 mL DMSO is then added to each well as a cell lysis solution. Percentage of cell viability is assessed by spectrophotometry at 570 nm^[1].

MedChemExpress (MCE) はこれらの方法の精度を確認していません。こちらは参照専用です。

参考文献

[1]. Mohammadi S, et al. Indole-3-carbinol induces G1 cell cycle arrest and apoptosis through aryl hydrocarbon receptor in THP-1 monocytic cell line.J Recept Signal Transduct Res. 2017 Oct;37(5):506-514. [Content Brief]

[2]. Safa M, et al. Indole-3-carbinol suppresses NF-κB activity and stimulates the p53 pathway in pre-B acute lymphoblastic leukemia cells. Tumour Biol. 2015 May;36(5):3919-30. [Content Brief]

[3]. Lee YR, et al. Reactivation of PTEN tumor suppressor for cancer treatment through inhibition of a MYC-WWP1 inhibitory pathway. Science. 2019 May 17;364(6441). pii: eaau0159. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.7944 mL	33.9720 mL	67.9440 mL	169.8600 mL
	5 mM	1.3589 mL	6.7944 mL	13.5888 mL	33.9720 mL
	10 mM	0.6794 mL	3.3972 mL	6.7944 mL	16.9860 mL
	15 mM	0.4530 mL	2.2648 mL	4.5296 mL	11.3240 mL
	20 mM	0.3397 mL	1.6986 mL	3.3972 mL	8.4930 mL
	25 mM	0.2718 mL	1.3589 mL	2.7178 mL	6.7944 mL
	30 mM	0.2265 mL	1.1324 mL	2.2648 mL	5.6620 mL
	40 mM	0.1699 mL	0.8493 mL	1.6986 mL	4.2465 mL
	50 mM	0.1359 mL	0.6794 mL	1.3589 mL	3.3972 mL
	60 mM	0.1132 mL	0.5662 mL	1.1324 mL	2.8310 mL
	80 mM	0.0849 mL	0.4247 mL	0.8493 mL	2.1233 mL
	100 mM	0.0679 mL	0.3397 mL	0.6794 mL	1.6986 mL

Indole-3-carbinol Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Indole-3-carbinol700-06-1Indole-3-methanolNF-κBAryl Hydrocarbon ReceptorE1/E2/E3 EnzymeEndogenous MetaboliteNuclear factor-κBNuclear factor-kappaBAhRE1 activating enzymeE2 conjugating enzymeE3 ligating enzymeUbiquitin activating enzymeUbiquitin conjugating enzymeUbiquitin ligaseInhibitorinhibitorinhibit

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Indole-3-carbinol (Synonyms: Indole-3-methanol; 3-インドールメタノール)

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生物活性

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純度とドキュメンテーション

参考文献

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Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Indole-3-carbinol Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

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Indole-3-carbinol (Synonyms: Indole-3-methanol; 3-インドールメタノール)

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Other Forms of Indole-3-carbinol:

Indole-3-carbinol 関連抗体

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おすすめ

•関連スクリーニングライブラリ:

•関連小分子:

•関連タンパク質:

NF-κB アイソフォーム固有の製品をすべて表示:

Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

生物活性

プロトコル

純度とドキュメンテーション

参考文献

カスタマーレビュー

Indole-3-carbinol 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Indole-3-carbinol Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

Keywords:

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