2. Metabolic Enzyme/Protease Anti-infection
  3. Cathepsin Ser/Thr Protease Virus Protease
  4. Leupeptin hemisulfate

Leupeptin (hemisulfate)  (Synonyms: ロイペプチンヘミ硫酸塩)

製品番号: HY-18234A 純度: 99.39%
COA 取扱説明書 Technical Support

Leupeptin hemisulfate is a broad-spectrum, membrane-permeable protease inhibitor. Leupeptin hemisulfate potently inhibits serine, cysteine and threonine proteases. Leupeptin hemisulfate inhibits Mpro (the main protease of SARS-CoV-2) and also has anti-inflammatory activity.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 103476-89-7

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 USD 63 在庫あり
Solution
10 mM * 1 mL in Water USD 63 在庫あり
Solid
5 mg $60 在庫あり
10 mg $84 在庫あり
25 mg $176 在庫あり
50 mg $285 在庫あり
100 mg $452 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 61 publication(s) in Google Scholar

Other Forms of Leupeptin hemisulfate:

Leupeptin (hemisulfate) 関連抗体

Top Publications Citing Use of Products

顧客検証

WB

    Leupeptin hemisulfate purchased from MedChemExpress. Usage Cited in: J Hematol Oncol. 2024 Sep 2;17(1):78.  [Abstract]

    The LTF expression was detected under MG132 (10 μM) or Leupeptin (100 μM) treatment.

    Leupeptin hemisulfate purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Feb;21(2):352-373.  [Abstract]

    Immunoblotting showing the level of PLCG1 in NPC after treatment with Leu (10 μM), NH4Cl (20 mM), or their combination for 12 h. Right panels represent normalized (/NC) band intensities of interest.

    Leupeptin hemisulfate purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Feb;21(2):352-373.  [Abstract]

    Western blot showing PLCG1 levels in NPC treated with a combination of Leu (10 μM) and NH4Cl (N/L) for 3, 6, and 9 h. Right panels represent normalized (/NC) band intensities of interest.

    Leupeptin hemisulfate purchased from MedChemExpress. Usage Cited in: Cell Death Differ. 2023 Jan;30(1):137-151.  [Abstract]

    Western blot analysis of SLC7A11, SOCS2 and tubulin proteins in SK-Hep-1 and HepG2 cells at 4 h after 4 Gy IR. Leupeptin (50 μM) were added before IR.

    Leupeptin hemisulfate purchased from MedChemExpress. Usage Cited in: Autophagy. 2021 Aug;17(8):1873-1888.  [Abstract]

    661 W cells were transfected with the indicated siRNA for 24 h. Then, 661 W cells were treated with 10 μg/ml Leupeptin for 24 h. Cell lysates were collected for western blot analysis of LC3B and GAPDH.

    Cathepsin アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Cathepsin B Cathepsin C Cathepsin D Cathepsin E Cathepsin K Cathepsin L Cathepsin S

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Leupeptin hemisulfate is a broad-spectrum, membrane-permeable protease inhibitor. Leupeptin hemisulfate potently inhibits serine, cysteine and threonine proteases. Leupeptin hemisulfate inhibits Mpro (the main protease of SARS-CoV-2) and also has anti-inflammatory activity[1][2][3].

    IC50 & Target

    Cathepsin B, Cathepsin H, Cathepsin L, Ser/Thr Protease, Mpro[1][2][3].
    体外実験

    Leupeptin hemisulfate (0.06-200 µM; 72 h) significantly decreases copy numbers of SARS-CoV-2 viral RNA (vRNA) in Vero cells[1]
    Leupeptin hemisulfate inhibits RNA levels of SARS-CoV-2 in Vero cells, with an EC50 value of 42.34 µM[1].
    Leupeptin hemisulfate has some inhibitory activity against Mpro, with an IC50 value of 127.2 µM[1].
    Leupeptin hemisulfate againsts human coronavirus strain 229E with an IC50 value of 0.4 µg/mL (about 1 µM)[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Leupeptin (hemisulfate) 関連抗体

    RT-PCR[1]

    Cell Line: Vero cells
    Concentration: 0.06-200 µM
    Incubation Time: 72 h
    Result: Significantly decreased copy numbers of SARS-CoV-2 viral RNA (vRNA).
    体内実験

    Leupeptin hemisulfate (0, 9, 18, 36 mg/kg; i.p.; single) is well tolerated by the animals and produces a strong, dose-dependent increase in LC3b-II in both the total tissue extracts and the lysosome and autophagosome-enriched pellet fraction[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: C57BL/6NCrl male mice (6-8 weeks old, 20-25 g)[1]
    Dosage: 0, 9, 18, 36 mg/kg
    Administration: Intraperitoneal injection; single
    Result: Promoted the accumulation of LC3b-II in mouse liver.
    分子量

    475.59

    分子式

    C20H38N6O4.1/2H2SO4
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    Sequence Shortening

    Ac-LLR-CHO
    SMILES

    O=C[C@@H](NC([C@@H](NC([C@@H](NC(C)=O)CC(C)C)=O)CC(C)C)=O)CCCNC(N)=N.[0.5H2SO4]
    Structure Classification
    Initial Source

    Microbial Metabolite
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    溶剤 & 溶解度
    体外: 

    H2O : 70 mg/mL (147.19 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1027 mL 10.5133 mL 21.0265 mL
    5 mM 0.4205 mL 2.1027 mL 4.2053 mL
    10 mM 0.2103 mL 1.0513 mL 2.1027 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (210.27 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.39%

    COA (250 KB) HNMR (272 KB) RP-HPLC (250 KB) MS (69 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 2.1027 mL 10.5133 mL 21.0265 mL 52.5663 mL
    5 mM 0.4205 mL 2.1027 mL 4.2053 mL 10.5133 mL
    10 mM 0.2103 mL 1.0513 mL 2.1027 mL 5.2566 mL
    15 mM 0.1402 mL 0.7009 mL 1.4018 mL 3.5044 mL
    20 mM 0.1051 mL 0.5257 mL 1.0513 mL 2.6283 mL
    25 mM 0.0841 mL 0.4205 mL 0.8411 mL 2.1027 mL
    30 mM 0.0701 mL 0.3504 mL 0.7009 mL 1.7522 mL
    40 mM 0.0526 mL 0.2628 mL 0.5257 mL 1.3142 mL
    50 mM 0.0421 mL 0.2103 mL 0.4205 mL 1.0513 mL
    60 mM 0.0350 mL 0.1752 mL 0.3504 mL 0.8761 mL
    80 mM 0.0263 mL 0.1314 mL 0.2628 mL 0.6571 mL
    100 mM 0.0210 mL 0.1051 mL 0.2103 mL 0.5257 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Leupeptin hemisulfate Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Leupeptin hemisulfate103476-89-7CathepsinSer/Thr ProteaseVirus ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesMproSARS-CoV-2Vero cellscathepsinserine proteasesthreonine proteasescysteine proteasesInhibitorinhibitorinhibit

    最近チェックした製品:

    オンラインお問い合わせ

    Your information is safe with us. * Required Fields.

    製品名

    		  

    カスタマ需要量 *

    お名前 *

    		 

    タイトル

    メールアドレス *

    		 

    電話番号 *

    デパートメント

    		 

    組纖名 *

    市区町村

    都道府県

    国或いは地域 *

    		      

    必ず会社名を記載ください。個人への返信は行いません。

    備考

    バルクお問い合わせ

    Inquiry Information

    製品名:
    Leupeptin hemisulfate
    製品番号:
    HY-18234A
    数量:
    MCE 日本正規代理店:

    カスタマーレビュー

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    製品名

    		  

    Lot #

    お名前 *

    		 

    メールアドレス *

    電話番号 *

    		 

    部門 *

    組纖名 *

    		 

    国或いは地域 *

    備考

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.