1. Cell Cycle/DNA Damage
    Epigenetics
  2. HDAC
  3. Oxamflatin

Oxamflatin (Synonyms: Metacept-3)

Cat. No.: HY-102033 Purity: 98.67%
Handling Instructions

Oxamflatin (Metacept-3) is a potent HDAC inhibitor with an IC50 of 15.7 nM.

For research use only. We do not sell to patients.

Oxamflatin Chemical Structure

Oxamflatin Chemical Structure

CAS No. : 151720-43-3

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10 mM * 1 mL in DMSO USD 163 In-stock
Estimated Time of Arrival: December 31
1 mg USD 72 In-stock
Estimated Time of Arrival: December 31
5 mg USD 216 In-stock
Estimated Time of Arrival: December 31
10 mg USD 336 In-stock
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25 mg USD 732 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

Oxamflatin (Metacept-3) is a potent HDAC inhibitor with an IC50 of 15.7 nM.

IC50 & Target[1]

HDAC

15.7 nM (IC50)

In Vitro

Oxamflatin induces transcriptional activation of junD and morphological reversion in various NIH3T3-derived transformed cell lines. Oxamflatin shows antiproliferative activity against various mouse and human tumor cell lines with drastic changes in the cell morphology. Oxamflatin causes an elongated cell shape with filamentous protrusions as well as arrest of the cell cycle at the G1 phase in HeLa cells. Oxamflatin greatly enhances the transcriptional activity of the CMV promoter in a dose-dependent manner and inhibits intracellular HDAC activity[1]. Oxamflatin in the nanomolar range induces morphological changes in OVCAR-5 and SKOV-3 ovarian cancer cell lines. Treatment with oxamflatin also leads to decreased cell viability. Oxamflatin is able to significantly inhibit DNA synthesis and cell proliferation[2]. Oxamflatin can induce E-cadherin expression and also reduce cell viability in the MKN-45 cell line[3].

In Vivo

Injection of oxamflatin, six times at the dose of 20 mg/kg, exhibits a significant increase in the days of survival (38% of ILS). The ILS of the mice treated with oxamflatin at the dose of 50 mg/kg is calculated to be more than 67% and one mouse survived over 60 days after tumor inoculation. No subsidiary effect, such as body weight loss, is observed at least up to this dose[1].

Molecular Weight

342.37

Formula

C₁₇H₁₄N₂O₄S

CAS No.

151720-43-3

SMILES

O=C(NO)/C=C/C#CC1=CC=CC(NS(=O)(C2=CC=CC=C2)=O)=C1

Shipping

Room temperature in continental US; may vary elsewhere

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 125 mg/mL (365.10 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9208 mL 14.6041 mL 29.2082 mL
5 mM 0.5842 mL 2.9208 mL 5.8416 mL
10 mM 0.2921 mL 1.4604 mL 2.9208 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

Cells grown in DMEM supplemented with 10% fetal bovine serum are challenged with serial two fold dilutions of oxamflatin on day 1 after the cells are seeded, and incubated for 2 days for the suspension cell cultures and for 3 days for the adherent cell cultures. Inhibition of the cell growth by oxamflatin is determined by staining with MTT as described previously[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Mice: Oxamflatin is injected intraperitoneally into BDF1 mice on day 1, 3, 5, 7, 9 and 11 and after the intraperitoneal inoculation of single cell suspension of the B16 melanoma cells. The survival days of the animals are recorded and the percent of increased life span (ILS%) is calculated[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Oxamflatin
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HY-102033
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