Oxamflatin (Synonyms: Metacept-3)

Cat. No.: HY-102033 Purity: 98.13%: FAQs
Data Sheet SDS COA Handling Instructions

Oxamflatin (Metacept-3) is a potent HDAC inhibitor with an IC₅₀ of 15.7 nM.

For research use only. We do not sell to patients.

Oxamflatin Chemical Structure

CAS No. : 151720-43-3

Size	Price	Stock	Quantity
Solution
10 mM * 1 mL in DMSO	USD 163	In-stock	Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL ready for reconstitution	USD 163	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 72	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 216	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 336	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 732	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Oxamflatin (Metacept-3) is a potent HDAC inhibitor with an IC₅₀ of 15.7 nM.

IC₅₀ & Target^[1]

HDAC

15.7 nM (IC₅₀)

In Vitro

Oxamflatin induces transcriptional activation of junD and morphological reversion in various NIH3T3-derived transformed cell lines. Oxamflatin shows antiproliferative activity against various mouse and human tumor cell lines with drastic changes in the cell morphology. Oxamflatin causes an elongated cell shape with filamentous protrusions as well as arrest of the cell cycle at the G1 phase in HeLa cells. Oxamflatin greatly enhances the transcriptional activity of the CMV promoter in a dose-dependent manner and inhibits intracellular HDAC activity^[1]. Oxamflatin in the nanomolar range induces morphological changes in OVCAR-5 and SKOV-3 ovarian cancer cell lines. Treatment with oxamflatin also leads to decreased cell viability. Oxamflatin is able to significantly inhibit DNA synthesis and cell proliferation^[2]. Oxamflatin can induce E-cadherin expression and also reduce cell viability in the MKN-45 cell line^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Injection of oxamflatin, six times at the dose of 20 mg/kg, exhibits a significant increase in the days of survival (38% of ILS). The ILS of the mice treated with oxamflatin at the dose of 50 mg/kg is calculated to be more than 67% and one mouse survived over 60 days after tumor inoculation. No subsidiary effect, such as body weight loss, is observed at least up to this dose^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

342.37

Appearance

Solid

Formula

C₁₇H₁₄N₂O₄S

CAS No.

151720-43-3

SMILES

O=C(NO)/C=C/C#CC1=CC=CC(NS(=O)(C2=CC=CC=C2)=O)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (146.04 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9208 mL	14.6041 mL	29.2082 mL
5 mM	0.5842 mL	2.9208 mL	5.8416 mL
10 mM	0.2921 mL	1.4604 mL	2.9208 mL

*Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (7.30 mM); Clear solution
2.

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (6.08 mM); Clear solution
3.

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: 2.08 mg/mL (6.08 mM); Suspended solution; Need ultrasonic

*All of the co-solvents are available by MCE.

Purity & Documentation

Purity: 98.13%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (245 KB) HNMR (261 KB) LCMS (268 KB)

Handling Instructions (2659 KB)

References

[1]. Kim YB, et al. Oxamflatin is a novel antitumor compound that inhibits mammalian histone deacetylase. Oncogene. 1999 Apr 15;18(15):2461-70. [Content Brief]

[2]. Wang YL, et al. HDAC Inhibitor Oxamflatin Induces Morphological Changes and has Strong Cytostatic Effects in Ovarian Cancer Cell Lines. Curr Mol Med. 2016;16(3):232-42. [Content Brief]

[3]. Faghihloo E, et al. The effect of oxamflatin on the E-cadherin expression in gastric cancer cell line. Cancer Gene Ther. 2016 Nov;23(11):396-399. [Content Brief]

Cell Assay ^[1]	Cells grown in DMEM supplemented with 10% fetal bovine serum are challenged with serial two fold dilutions of oxamflatin on day 1 after the cells are seeded, and incubated for 2 days for the suspension cell cultures and for 3 days for the adherent cell cultures. Inhibition of the cell growth by oxamflatin is determined by staining with MTT as described previously^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Mice: Oxamflatin is injected intraperitoneally into BDF1 mice on day 1, 3, 5, 7, 9 and 11 and after the intraperitoneal inoculation of single cell suspension of the B16 melanoma cells. The survival days of the animals are recorded and the percent of increased life span (ILS%) is calculated^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Kim YB, et al. Oxamflatin is a novel antitumor compound that inhibits mammalian histone deacetylase. Oncogene. 1999 Apr 15;18(15):2461-70. [Content Brief] [2]. Wang YL, et al. HDAC Inhibitor Oxamflatin Induces Morphological Changes and has Strong Cytostatic Effects in Ovarian Cancer Cell Lines. Curr Mol Med. 2016;16(3):232-42. [Content Brief] [3]. Faghihloo E, et al. The effect of oxamflatin on the E-cadherin expression in gastric cancer cell line. Cancer Gene Ther. 2016 Nov;23(11):396-399. [Content Brief]

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Keywords:

Oxamflatin151720-43-3Metacept-3Metacept3Metacept 3HDACHistone deacetylasesInhibitorinhibitorinhibit

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Oxamflatin (Synonyms: Metacept-3)

Customer Review

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References

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