2. GPCR/G Protein Neuronal Signaling Autophagy
  3. Adrenergic Receptor Autophagy
  4. Prazosin hydrochloride

プラゾシン塩酸塩  (Synonyms: Prazosin (hydrochloride))

製品番号: HY-B0193A 純度: 99.89%
COA 取扱説明書 Technical Support

Prazosin hydrochloride is a well-tolerated, CNS-active α1-adrenergic receptor antagonist for the research of high blood pressure and alcohol use disorders. Prazosin hydrochloride potently inhibits Norepinephrine (NE)-stimulated 45Ca efflux with an IC50 of 0.15 nM.Prazosin hydrochloride inhibits organic cation transporters OCT-1 and OCT-3 with IC50s of 1.8, and 13 μM, respectively.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 19237-84-4

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 72 在庫あり
Solution
10 mM * 1 mL in DMSO USD 72 在庫あり
Solid
100 mg $66 在庫あり
200 mg $95 在庫あり
500 mg $190 在庫あり
1 g   お問い合わせ  
5 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 17 publication(s) in Google Scholar

Other Forms of Prazosin hydrochloride:

プラゾシン塩酸塩 関連抗体

Top Publications Citing Use of Products

    Prazosin hydrochloride purchased from MedChemExpress. Usage Cited in: Redox Rep. 2025 Dec;30(1):2494314.  [Abstract]

    Prazosin (10 μM) abolished NE-induced increases in NOX2 expression in VAFs of Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR).

    Prazosin hydrochloride purchased from MedChemExpress. Usage Cited in: BMC Pulm Med. 2021 Jun 5;21(1):189.  [Abstract]

    Prazosin (2 mg/kg; i.p.; 60 min) abolished the inhibition of PAVA on TNF-α production in C57BL/6J male mice.

    Prazosin hydrochloride purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2020 Apr;124:109731.  [Abstract]

    The expression of TNS1 in U937 and HL60 cells treated with different concentration of Prazosin is detected by Western blot analysis

    Prazosin hydrochloride purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2020 Apr;124:109731.  [Abstract]

    Prazosin (5-15 μM) promoted apoptosis in HL60 and U937 cells in a dose-dependent manner under serum deprivation.

    Prazosin hydrochloride purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2020 Apr;124:109731.  [Abstract]

    Cell proliferation was measured by the CCK-8 assay in U937 and HL60 cells treated with Prazosin (5-15 μM).

    Prazosin hydrochloride purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2020 Apr;124:109731.  [Abstract]

    Prazosin (15 μM; 24 h) down-regulated the expression of all candidate genes except ABCD1 in U937 cells, with TNS1 being the most significantly down-regulated.

    Adrenergic Receptor アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    α adrenergic receptor β adrenergic receptor

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Prazosin hydrochloride is a well-tolerated, CNS-active α1-adrenergic receptor antagonist for the research of high blood pressure and alcohol use disorders[1]. Prazosin hydrochloride potently inhibits Norepinephrine (NE)-stimulated 45Ca efflux with an IC50 of 0.15 nM[2].Prazosin hydrochloride inhibits organic cation transporters OCT-1 and OCT-3 with IC50s of 1.8, and 13 μM, respectively[3].

    IC50 & Target

    α adrenergic receptor

     

    体外実験

    Prazosin (0, 2.5, 5, 7.5, 10, 15, 20, 30, 40 and 50 μM) effectively inhibits the proliferation of U251 and U87 cells[4].
    Prazosin inhibits the migration and invasion of U251 and U87 cells[4].
    Prazosin treatment decreases the protein expression of components of the PI3K/AKT/mTOR signaling pathway. Prazosin (13.16 and 11.57 μM for U251 and U87 cells, 48 hours) decreases the expression levels of P70 and cyclin D1, which are downstream target genes of the PI3K/AKT/mTOR signaling pathway[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    プラゾシン塩酸塩 関連抗体

    Cell Proliferation Assay

    Cell Line: U251 and U87 cells
    Concentration: 0, 2.5, 5, 7.5, 10, 15, 20, 30, 40 and 50 µM.
    Incubation Time: 48 hours
    Result: The IC50s were 13.16±0.95 and 11.57±0.79 µM for U251 and U87 cells, respectively.

    Western Blot Analysis

    Cell Line: U251 and U87 cells
    Concentration: 13.16 and 11.57 µM for U251 and U87 cells, respectively
    Incubation Time: 48 hours
    Result: Protein expression levels of Bax and active Caspase-3 were increased.
    Bcl-2 levels were also decreased after prazosin treatment (P<0.05).
    The expression of p-AKT and p-mTOR, P70 and cyclin D1 were decreased.
    体内実験

    Peripheral administration of Prazosin (0, 0.5, 1.0, 1.5 or 2.0 mg/kg; i.p.) can suppress not only central α1-adrenergic-mediated hyperexcitability but also stress-induced anxiety[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Fifty-five alcohol-naive male rats from the 60th generation of selective breeding for alcohol preference[1]
    Dosage: 0.5, 1.0, 1.5, or 2.0 mg/kg
    Administration: Injected intraperitoneally (IP); 0.5 mg/mL; once a day at 15 min prior to onset of the daily two hour two-bottle choice, alcohol versus water, access period for two consecutive days and then three weeks later for five consecutive days.
    Result: Significantly reduced alcohol intake during the initial two daily administrations, and this reduction of alcohol intake was maintained for five consecutive days by daily prazosin treatment in the subsequent more prolonged trial.
    臨床実験
    分子量

    419.86

    分子式

    C19H22ClN5O4
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(N1CCN(C2=NC(N)=C3C=C(OC)C(OC)=CC3=N2)CC1)C4=CC=CO4.Cl
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    溶剤 & 溶解度
    体外: 

    DMSO : 10 mg/mL (23.82 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 0.59 mg/mL (1.41 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.3817 mL 11.9087 mL 23.8175 mL
    5 mM 0.4763 mL 2.3817 mL 4.7635 mL
    10 mM 0.2382 mL 1.1909 mL 2.3817 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 0.83 mg/mL (1.98 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 0.83 mg/mL (1.98 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (8.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.89%

    COA (250 KB) HNMR (263 KB) RP-HPLC (152 KB) LCMS (334 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture). When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 2.3817 mL 11.9087 mL 23.8175 mL 59.5437 mL
    DMSO 5 mM 0.4763 mL 2.3817 mL 4.7635 mL 11.9087 mL
    10 mM 0.2382 mL 1.1909 mL 2.3817 mL 5.9544 mL
    15 mM 0.1588 mL 0.7939 mL 1.5878 mL 3.9696 mL
    20 mM 0.1191 mL 0.5954 mL 1.1909 mL 2.9772 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Prazosin hydrochloride Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Prazosin19237-84-4Adrenergic ReceptorAutophagyBeta Receptoradrenergicreceptorhighbloodpressurealcoholuse disordersNorepinephrine45CaeffluxorganiccationtransportersOCT-1OCT-3Inhibitorinhibitorinhibit

    最近チェックした製品:

    オンラインお問い合わせ

    Your information is safe with us. * Required Fields.

    製品名

    		  

    カスタマ需要量 *

    お名前 *

    		 

    タイトル

    メールアドレス *

    		 

    電話番号 *

    デパートメント

    		 

    組纖名 *

    市区町村

    都道府県

    国或いは地域 *

    		      

    必ず会社名を記載ください。個人への返信は行いません。

    備考

    バルクお問い合わせ

    Inquiry Information

    製品名:
    Prazosin hydrochloride
    製品番号:
    HY-B0193A
    数量:
    MCE 日本正規代理店:

    カスタマーレビュー

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    製品名

    		  

    Lot #

    お名前 *

    		 

    メールアドレス *

    電話番号 *

    		 

    部門 *

    組纖名 *

    		 

    国或いは地域 *

    備考

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.