Sarsasapogenin (Synonyms: Parigenin; Sarsagenin)

Name: Sarsasapogenin
Brand: MedChemExpress
SKU: HY-N0073
Rating: 5.0 (4 reviews)

Cat. No.: HY-N0073 Purity: 99.93%: Data Sheet
COA

SDS
Handling Instructions
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Sarsasapogenin is a sapogenin from the Chinese medical herb Anemarrhena asphodeloides Bunge, with antidiabetic, anti-oxidative, anticancer and anti-inflamatory activities.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Sarsasapogenin 화학구조

CAS No. : 126-19-2

사이즈	재고	수량
무료 샘플 (0.1 - 0.2 mg)	지금 신청하기
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

or 대량구매 문의

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This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 4 publication(s) in Google Scholar

Other Forms of Sarsasapogenin:

Sarsasapogenin (Standard) In-stock
Spirostan-3-ol 견적 받기

4 Publications Citing Use of MCE Sarsasapogenin

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View All Endogenous Metabolite Isoform Specific Products:

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Human Endogenous Metabolite Microbial Metabolite

View All NF-κB Isoform Specific Products:

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

Biological Activity
Protocol
순도&문서
References
고객리뷰

제품 설명

Sarsasapogenin is a sapogenin from the Chinese medical herb Anemarrhena asphodeloides Bunge, with antidiabetic, anti-oxidative, anticancer and anti-inflamatory activities.

IC₅₀ & Target^[3]

NF-κB

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	38.15 μM Compound: Sarsasapogenin	Cytotoxicity against human A549 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay Cytotoxicity against human A549 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay	[PMID: 28038327]
Bcap37	IC₅₀	> 100 μM Compound: Sarsasapogenin	Cytotoxicity against human Bcap37 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay Cytotoxicity against human Bcap37 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay	[PMID: 28038327]
Hep 3B2	IC₅₀	> 100 μM Compound: Sarsasapogenin	Cytotoxicity against human Hep3B cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay Cytotoxicity against human Hep3B cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay	[PMID: 28038327]
HepG2	IC₅₀	40.34 μM Compound: Sarsasapogenin	Cytotoxicity against human HepG2 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay Cytotoxicity against human HepG2 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay	[PMID: 28038327]
MCF7	IC₅₀	49.23 μM Compound: Sarsasapogenin	Cytotoxicity against human MCF7 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay Cytotoxicity against human MCF7 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay	[PMID: 28038327]
THP-1	IC₅₀	3.3 μM Compound: 3	Anticoagulant activity in human THP1 cells assessed as inhibition of TNF-alpha-stimulated tissue factor expression incubated 1 hr before TNFalpha challenge measured after 5 hrs Anticoagulant activity in human THP1 cells assessed as inhibition of TNF-alpha-stimulated tissue factor expression incubated 1 hr before TNFalpha challenge measured after 5 hrs	[PMID: 20561785]
U-251	IC₅₀	> 100 μM Compound: Sarsasapogenin	Cytotoxicity against human U251 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay Cytotoxicity against human U251 cells assessed as decrease in cell proliferation after 48 hrs by MTT colorimetric assay	[PMID: 28038327]

In Vitro

Sarsasapogenin (20-80 μM) induces apoptosis via caspase-dependent mitochondrial pathway in heLa cells. Sarsasapogenin also induces apoptosis via the caspase-dependent mitochondrial apoptotic pathway. Sarsasapogenin (60 μM)-induced ROS generation triggers ER stress and mitochondrial dysfunction^[1]. Sarsasapogenin potently inhibits NF-κB and MAPK activation, as well as IRAK1, TAK1, and IκBα phosphorylation in LPS-stimulated macrophages. Moreover, Sarsasapogenin inhibits the binding of LPS to macrophage Toll-like receptor 4, and polarization of M2 to M1 macrophages^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Sarsasapogenin (20 and 40 mg/kg) significantly restores the sucrose preference deficit induced by olfactory bulbectomy (OB), and increases locomotor activity. Sarsasapogenin groups (20 and 40 mg/kg) have significantly lower immobility times, higher AChE protein expression levels than the OB group. Furthermore, Sarsasapogenin (20 and 40 mg/kg) groups have significantly higher α7-nAChR protein expression, and increases higher α4-nAChR protein expression levels compared to rats in the OB group^[2]. Sarsasapogenin (5 or 10 mg/kg, p.o.) inhibits TNBS-induced colon shortening and myeloperoxidase activity in mice, reducing NF-κB activation and interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and IL-6 levels, while simultaneously increasing IL-10^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

416.64

화학식

C₂₇H₄₄O₃

CAS No.

126-19-2

Appearance

Solid

Color

White to off-white

SMILES

C[C@@H]1[C@]2(OC[C@@H](C)CC2)O[C@@]3([H])C[C@@]4([H])[C@]5([H])CC[C@]6([H])C[C@@H](O)CC[C@]6(C)[C@@]5([H])CC[C@]4(C)[C@]31[H]

Structure Classification

Steroids

Initial Source

Endogenous metabolite

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

용액&용해도

In Vitro:

DMSO : 3.57 mg/mL (8.57 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 1 mg/mL (2.40 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4002 mL	12.0008 mL	24.0015 mL
5 mM	0.4800 mL	2.4002 mL	4.8003 mL
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC-Na/saline water
Solubility: 5 mg/mL (12.00 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

순도&문서

Purity: 99.93%

Select Batch:

Data Sheet (281 KB) SDS (393 KB)

COA (251 KB) HNMR (296 KB) RP-HPLC (242 KB) MS (71 KB)

Handling Instructions (2659 KB)

References

[1]. Shen S, et al. Sarsasapogenin induces apoptosis via the reactive oxygen species-mediated mitochondrial pathway and ER stress pathway in HeLa cells. Biochem Biophys Res Commun. 2013 Oct 26.

[2]. Feng B, et al. Sarsasapogenin reverses depressive-like behaviors and nicotinic acetylcholine receptors induced by olfactory bulbectomy. Neurosci Lett. 2017 Feb 3;639:173-178. [Content Brief]

[3]. Lim SM, et al. Timosaponin AIII and its metabolite sarsasapogenin ameliorate colitis in mice by inhibiting NF-κB and MAPK activation and restoring Th17/Treg cell balance. Int Immunopharmacol. 2015 Apr;25(2):493-503. [Content Brief]

Cell Assay ^[1]	The effect of Sarsasapogenin on cell viability is measured by the MTT assay method. After exposure to Sarsasapogenin in the absence or presence of Z-VAD-FMK pretreatment for the indicated time, cells in 96-well plates are incubated with MTT. The formazan precipitate is dissolved in 200 μL of dimethyl sulfoxide, and the absorbance at 570 nm is measured using a Benchmark microplate reader. MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.
Animal Administration ^[3]	The mice are randomly divided into 7 groups: normal control, TNBS-induced colitic control groups treated with vehicle, timosaponin AIII (5 or 10 mg/kg), Sarsasapogenin (5 or 10 mg/kg) or sulfasalazine (50 mg/kg). Each group consists of 6 mice. The colitis is induced by the intrarectal injection of 2.5% (w/v) TNBS solution (100 μL, dissolved in 50% ethanol) into the colon of anesthetized mice using a thin round-tip needle equipped with a syringe. The normal group is treated with vehicle alone instead of TNBS and test agents. If the injected TNBS solution is excreted, the mouse is excluded from the experiment. Timosaponin AIII or Sarsasapogenin (5 or 10 mg/kg) or sulfasalazine (50 mg/kg) dissolved in 2% tween 80 are orally administered once a day for 3 days after treatment with TNBS. The mice are killed 18 h after the final administration of test agents. The colon is quickly removed, opened longitudinally, and gently washed by PBS. Macroscopic evaluation of the colitis grade is scored (0, no ulcer and no inflammation; 1, no ulceration and local hyperemia; 2, ulceration with hyperemia; 3, ulceration and inflammation at one site only; 4, two or more sites of ulceration and inflammation; 5, ulceration extending more than 2 cm). The colons are stored at −80°C until used in the experiment for ELISA and immunoblotting. MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.
References	[1]. Shen S, et al. Sarsasapogenin induces apoptosis via the reactive oxygen species-mediated mitochondrial pathway and ER stress pathway in HeLa cells. Biochem Biophys Res Commun. 2013 Oct 26. [2]. Feng B, et al. Sarsasapogenin reverses depressive-like behaviors and nicotinic acetylcholine receptors induced by olfactory bulbectomy. Neurosci Lett. 2017 Feb 3;639:173-178. [Content Brief] [3]. Lim SM, et al. Timosaponin AIII and its metabolite sarsasapogenin ameliorate colitis in mice by inhibiting NF-κB and MAPK activation and restoring Th17/Treg cell balance. Int Immunopharmacol. 2015 Apr;25(2):493-503. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	2.4002 mL	12.0008 mL	24.0015 mL	60.0038 mL
DMSO	5 mM	0.4800 mL	2.4002 mL	4.8003 mL	12.0008 mL

Sarsasapogenin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sarsasapogenin126-19-2Parigenin SarsageninEndogenous MetaboliteNF-κBNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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Sarsasapogenin (Synonyms: Parigenin; Sarsagenin)

고객리뷰

Other Forms of Sarsasapogenin:

Sarsasapogenin Related Antibodies

4 Publications Citing Use of MCE Sarsasapogenin

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

View All NF-κB Isoform Specific Products:

Biological Activity

Protocol

순도&문서

References

고객리뷰

Sarsasapogenin Related Antibodies

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

Sarsasapogenin Related Classifications

Keywords:

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고객리뷰

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report