2. PI3K/Akt/mTOR Stem Cell/Wnt
  3. GSK-3
  4. Tideglusib

Tideglusib  (Synonyms: チデグルシブ; NP031112)

製品番号: HY-14872 純度: 99.70%
COA 取扱説明書 Technical Support

Tideglusib (NP031112) is an irreversible GSK-3 inhibitor with IC50s of 5 nM and 60 nM for GSK-3βWT (1 h preincubation) and GSK-3βC199A (1 h preincubation), respectively.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 865854-05-3

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 35 在庫あり
Solution
10 mM * 1 mL in DMSO USD 35 在庫あり
Solid
5 mg $32 在庫あり
10 mg $50 在庫あり
25 mg $95 在庫あり
50 mg $150 在庫あり
100 mg $250 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 7 publication(s) in Google Scholar

Tideglusib 関連抗体

Top Publications Citing Use of Products

    Tideglusib purchased from MedChemExpress. Usage Cited in: Mol Cell Biochem. 2026 Jan 29.  [Abstract]

    Western blot analysis of p-GSK3β and GSK3β in BEAS-2B and MLE-12 cells treated under indicated conditions: No EF, EFs (100 mV/mm, 2 h), and EFs (100 mV/mm, 2 h)+Tideglusib (TID, 20 µM, 24 h), with quantification of p-GSK3β/GSK3β ratio.

    Tideglusib purchased from MedChemExpress. Usage Cited in: Mol Cell Biochem. 2026 Jan 29.  [Abstract]

    Western blot analysis of p-GSK3β and GSK3β in BEAS-2B and MLE-12 cells treated under indicated conditions: No EF, EFs (100 mV/mm, 2 h), and EFs (100 mV/mm, 2 h)+Tideglusib (TID, 20 µM, 24 h), with quantification of p-GSK3β/GSK3β ratio.

    Tideglusib purchased from MedChemExpress. Usage Cited in: Mol Cell Biochem. 2026 Jan 29.  [Abstract]

    Immunofluorescence analysis of Ki-67 in MLE-12 cells treated under indicated conditions: No EF, Tideglusib (TID; 20 µM, 24 h), EFs (100 mV/mm, 2 h), and EFs (100 mV/mm, 2 h)+Tideglusib (TID; 20 µM, 24 h), with quantification of Ki-67 positive cells.

    Tideglusib purchased from MedChemExpress. Usage Cited in: Mol Cell Biochem. 2026 Jan 29.  [Abstract]

    Immunofluorescence analysis of Ki-67 in BEAS2B cells treated under indicated conditions: No EF, Tideglusib (TID; 20 μM, 24 h), EFs (100 mV/mm, 2 h), and EFs (100 mV/mm, 2 h) + TID(20 μM, 24 h), with quantification of Ki-67 positive cells.

    Tideglusib purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2025 Oct 30:242:275-287.  [Abstract]

    HuH7 cells were treated with 7,8-DHF (5 μM), Tideglusib (10 μM) and concentration gradient RSL3 (0-10 μM) for 24 h. Cell viability was measured by CCK-8 assay.

    Tideglusib purchased from MedChemExpress. Usage Cited in: Int J Clin Exp Pathol. 2017;10(3):3033-3042.

    Response of 6 HCC cell lines to 12 antitumor agents (Tideglusib; 0-10μM). The IC50 values were determined by MTS assay and calculated with the aid of GraphPad Prism 5.0 software by nonlinear regression.

    Tideglusib purchased from MedChemExpress. Usage Cited in: Int J Clin Exp Pathol. 2017;10(3):3033-3042.

    Response of 6 HCC cell lines to 12 antitumor agents (Tideglusib; 0-10μM).

    GSK-3 アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    GSK-3 GSK-3α GSK-3β

    • 生物活性

    • プロトコル

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Tideglusib (NP031112) is an irreversible GSK-3 inhibitor with IC50s of 5 nM and 60 nM for GSK-3βWT (1 h preincubation) and GSK-3βC199A (1 h preincubation), respectively.

    IC50 & Target[1]

    GSK-3β(WT)

    5 nM (IC50)

    GSK-3β(C199A)

    60 nM (IC50)

    Cellular Effect
    Cell Line Type Value Description References
    Vero C1008 IC50
    0.67 μM
    Compound: 13
    Antiviral activity against SARS-CoV-2 infected in African green monkey Vero E6 cells assessed as reduction in cell growth
    Antiviral activity against SARS-CoV-2 infected in African green monkey Vero E6 cells assessed as reduction in cell growth
    		[PMID: 37244162]
    体外実験

    Incubation of both astrocyte and microglial cultures with Tideglusib (NP031112) completely abrogates the induction of TNF-α and COX-2 expression after glutamate treatment. These effects of NP031112 are not caused by a loss of cell viability, because the 24 h exposure of astrocyte and microglial cells to this TDZD does not modify cell viability[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Tideglusib 関連抗体
    体内実験

    Injection of Tideglusib (NP031112) (50 mg/kg) into the rat hippocampus dramatically reduces kainic acid-induced inflammation, as measured by edema formation using T2-weighted magnetic resonance imaging and glial activation and has a neuroprotective effect in the damaged areas of the hippocampus[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    臨床実験
    分子量

    334.39

    分子式

    C19H14N2O2S
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(N(SC1=O)C2=C3C=CC=CC3=CC=C2)N1CC4=CC=CC=C4
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 33.33 mg/mL (99.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.9905 mL 14.9526 mL 29.9052 mL
    5 mM 0.5981 mL 2.9905 mL 5.9810 mL
    10 mM 0.2991 mL 1.4953 mL 2.9905 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (7.48 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (7.48 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.70%

    COA (262 KB) HNMR (266 KB) RP-HPLC (222 KB) MS (70 KB)

    取扱説明書 (2659 KB)
    参考文献
    動物実験
    [2]

    Rats[2]
    Adult male Wistar rats (8-12 weeks old) are used in this study. Rats (n≥5 per group) are placed into a stereotaxic apparatus. KA (1 μg in 2.5 μL PBS) alone or in combination with Tideglusib (2 ng in 2.5 μL PBS) is injected into the hippocampus. Control animals of the same age are injected with vehicle.

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.9905 mL 14.9526 mL 29.9052 mL 74.7630 mL
    5 mM 0.5981 mL 2.9905 mL 5.9810 mL 14.9526 mL
    10 mM 0.2991 mL 1.4953 mL 2.9905 mL 7.4763 mL
    15 mM 0.1994 mL 0.9968 mL 1.9937 mL 4.9842 mL
    20 mM 0.1495 mL 0.7476 mL 1.4953 mL 3.7381 mL
    25 mM 0.1196 mL 0.5981 mL 1.1962 mL 2.9905 mL
    30 mM 0.0997 mL 0.4984 mL 0.9968 mL 2.4921 mL
    40 mM 0.0748 mL 0.3738 mL 0.7476 mL 1.8691 mL
    50 mM 0.0598 mL 0.2991 mL 0.5981 mL 1.4953 mL
    60 mM 0.0498 mL 0.2492 mL 0.4984 mL 1.2460 mL
    80 mM 0.0374 mL 0.1869 mL 0.3738 mL 0.9345 mL
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    Tideglusib Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Tideglusib865854-05-3NP031112NP 031112NP-031112GSK-3Glycogen synthase kinase-3Glycogen synthase kinase 3Inhibitorinhibitorinhibit

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    製品名:
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    製品番号:
    HY-14872
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