1. Cell Cycle/DNA Damage
    Stem Cell/Wnt
    Cytoskeleton
    TGF-beta/Smad
  2. ROCK
  3. Y-27632 dihydrochloride

Y-27632 dihydrochloride 

製品番号: HY-10583 純度: 99.98%
COA 取扱説明書

Y-27632 dihydrochloride is an orally active and ATP-competitive ROCK (Rho-kinase) inhibitor (ROCK-I Ki=220 nM; ROCK-II Ki=300 nM). Y-27632 dihydrochloride shows antiepileptic effects.

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Y-27632 dihydrochloride 構造式

Y-27632 dihydrochloride 構造式

CAS 番号 : 129830-38-2

容量 価格(税別) 在庫状況 数量
無料サンプル (0.5-1 mg)   今すぐ申し込む  
Solution
10 mM * 1 mL in DMSO USD 55 在庫あり
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 55 在庫あり
Estimated Time of Arrival: December 31
Solid
10 mg USD 70 在庫あり
Estimated Time of Arrival: December 31
50 mg USD 210 在庫あり
Estimated Time of Arrival: December 31
100 mg USD 350 在庫あり
Estimated Time of Arrival: December 31
200 mg USD 550 在庫あり
Estimated Time of Arrival: December 31
500 mg USD 1150 在庫あり
Estimated Time of Arrival: December 31
1 g USD 1650 在庫あり
Estimated Time of Arrival: December 31
2 g USD 2650 在庫あり
Estimated Time of Arrival: December 31
5 g   お問い合わせ  
10 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

カスタマーレビュー

Based on 137 publication(s) in Google Scholar

Other Forms of Y-27632 dihydrochloride:

Top Publications Citing Use of Products

顧客検証

WB
IF

    Y-27632 dihydrochloride purchased from MCE. Usage Cited in: J Exp Clin Cancer Res. 2018 Jun 28;37(1):128.  [Abstract]

    MHCC-97H and Huh-7 cells co-transfected with control or supervillin-specific siRNA and a RhoA(WT), RhoA(V14), or RhoA(N19) plasmids for 48 h are treated with PBS or Y27632 2HCl (10 μM) for 16 h during hypoxia, and then assayed for phosphorylated p38 and ERK by immunoblotting. β-actin is used as the loading control

    Y-27632 dihydrochloride purchased from MCE. Usage Cited in: Oncotarget. 2017 Jul 31;8(47):82506-82530.  [Abstract]

    SKOV3 mock-transfected control (Ctrl) and shRNA-Hic-5 knockdown (KD) (sh-S1 and sh-S2) cells were either untreated (-) or treated (+) with 10mM Y27632 over a period of 48 hr. SKOV3 mock-transfected control (Ctrl) and shRNA-Hic-5 knockdown (KD) (sh-S1 and sh-S2) clones were either untreated (-) or treated (+) with 10 mM Y27632 followed by stimulation without (-) or with (+) 5 ng/mL of TGFβ1 for 48 hr.

    Y-27632 dihydrochloride purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2020 Jun 17;7(15):1903583.  [Abstract]

    Immunofluorescence imaging of LNCaP cells treated with the Cytoskeleton B (CB), Nocodazole (NO), PF-573288 (PF), Y-27632 (Y), and (-)-Blebbistatin ((-) Bl). After the cells are cultured on different substrates for 3 days, inhibitors are added and incubated for 24 h.

    Y-27632 dihydrochloride purchased from MCE. Usage Cited in: Adv Sci (Weinh). 2020 Jun 17;7(15):1903583.  [Abstract]

    Expression of CTC cluster-related (plakoglobin and CD44) and EMT-related (vimentin and E-cadherin, 4 days) proteins in LNCaP cells grown on different substrates after treatment with different inhibitors (Cytoskeleton B (CB), Nocodazole (NO), PF-573288 (PF), Y-27632 (Y), and (-)-Blebbistatin ((-) Bl)).

    ROCK アイソフォーム固有の製品をすべて表示:

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Y-27632 dihydrochloride is an orally active and ATP-competitive ROCK (Rho-kinase) inhibitor (ROCK-I Ki=220 nM; ROCK-II Ki=300 nM). Y-27632 dihydrochloride shows antiepileptic effects[1][2][3][4].

    IC50 & Target[1]

    ROCK-I

    220 nM (Ki)

    ROCK-II

    300 nM (Ki)

    PKN

    3.1 μM (Ki)

    Citron kinase

    5.3 μM (Ki)

    PKCα

    73 μM (Ki)

    PKA

    25 μM (Ki)

    体外実験

    Y-27632 (1-5 μM; 0-60 min) promotes neuronal differentiation of adipose tissue-derived stem cells (ADSCs) [3].
    Y-27632 (1-5 μM; 0-60 min) induces the expression of NSE, MAP-2 and nestin in ADSCs [3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Western Blot Analysis[3]

    Cell Line: Adipose tissue-derived stem cells (ADSCs)
    Concentration: 20 μM
    Incubation Time: 24 hours
    Result: Resulted in the up-regulation of NSE, MAP-2 and nestin protein levels by 25.3, 3.1 and 2.5 fold, respectively, compared to control cells not treated by Y-27632.
    体内実験

    Y-27632 (oral gavage; 30 mg/kg; once daily; 4 w) prevents dimethylnitrosamine-induced hepatic fibrosis in rats[1].
    Y-27632 (oral gavage; 5-10 mg/kg; once) shows antiepileptic effects in epilepsy induced by PTZ and MES[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male Wistar rats injected with dimethylnitrosamine[1]
    Dosage: 30 mg/kg
    Administration: Oral gavage; 30 mg/kg; once daily; 4 weeks
    Result: Decreased the occurrence of dimethylnitrosamine-induced hepatic fibrosis and reduced the collagen and hydroxyproline content and α-smooth muscle actin expression in the liver.
    Animal Model: Male Swiss albino mice injected with PTZ (pentylenetetrazole) or induced by MES (maximal electroconvulsive shock)[2]
    Dosage: 5-10 mg/kg
    Administration: Oral gavage; 5-10 mg/kg; once
    Result: Prolonged the onset time of myoclonic jerks when compared with those observed in the saline group (P<0.05).
    Prolonged the onset time of clonic convulsions when compared with saline group (P<0.05).
    Prevented the occurrence of tonic hindlimb extensions and death.
    分子量

    320.26

    分子式

    C14H23Cl2N3O

    CAS 番号
    SMILES

    O=C([[email protected]@H]1CC[[email protected]]([[email protected]](N)C)([H])CC1)NC2=CC=NC=C2.[H]Cl.[H]Cl

    輸送条件

    Room temperature in continental US; may vary elsewhere.

    保管条件

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶剤 & 溶解度
    体外: 

    H2O : 100 mg/mL (312.25 mM; Need ultrasonic)

    DMSO : 33.33 mg/mL (104.07 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.1225 mL 15.6123 mL 31.2246 mL
    5 mM 0.6245 mL 3.1225 mL 6.2449 mL
    10 mM 0.3122 mL 1.5612 mL 3.1225 mL
    *Please refer to the solubility information to select the appropriate solvent.
    体内:
    • 1.

      Add each solvent one by one:  PBS

      Solubility: 220 mg/mL (686.94 mM); Clear solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (7.81 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (7.81 mM); Clear solution

    • 4.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (7.81 mM); Clear solution

    • 5.

      Add each solvent one by one:  5% DMSO    40% PEG300    5% Tween-80    50% saline

      Solubility: ≥ 1.25 mg/mL (3.90 mM); Clear solution

    • 6.

      Add each solvent one by one:  5% DMSO    95% (20% SBE-β-CD in saline)

      Solubility: ≥ 1.25 mg/mL (3.90 mM); Clear solution

    *All of the co-solvents are available by MCE.
    純度とドキュメンテーション

    純度: 99.98%

    参考文献
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    製品名:
    Y-27632 dihydrochloride
    製品番号:
    HY-10583
    数量:
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