2. Apoptosis
  3. Caspase
  4. Ac-YVAD-cmk

Ac-YVAD-cmk  (Synonyms: Caspase-1 Inhibitor II)

製品番号: HY-16990 純度: 99.63%
COA 取扱説明書 Technical Support

Ac-YVAD-cmk (Caspase-1 Inhibitor II) is a selective caspase-1 (IL-1beta converting enzyme, ICE)) inhibitor with neuroprotective and anti-inflammatory effects. Ac-YVAD-cmk effectively suppresses the expression of IL-1β and IL-18. Ac-YVAD-cmk inhibits pyroptosis in many diseases.

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CAS 番号 : 178603-78-6

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 333 在庫あり
Solution
10 mM * 1 mL in DMSO USD 333 在庫あり
Solid
1 mg $80 在庫あり
5 mg $280 在庫あり
10 mg $450 在庫あり
25 mg $735 在庫あり
50 mg $1040 在庫あり
100 mg $1450 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 34 publication(s) in Google Scholar

Ac-YVAD-cmk 関連抗体

Top Publications Citing Use of Products

顧客検証

ELISA
IF
WB
Histological Imaging/Staining

    Ac-YVAD-cmk purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 2;16(1):6074.  [Abstract]

    The concentration of IL-18 in cell culture supernatants of MCs after stimulation with or without LPS and Nig in the presence or absence of Ac-YVAD-cmk (50 μM/mL).

    Ac-YVAD-cmk purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 2;16(1):6074.  [Abstract]

    Immunoblot analysis of cell lysates of LUVA cell line after stimulation with or without LPS and Nig in the presence or absence of Ac-YVAD-cmk (50 μM/mL).

    Ac-YVAD-cmk purchased from MedChemExpress. Usage Cited in: Bone Res. 2024 Feb 22;12(1):11.  [Abstract]

    Immunofluorescence staining of N-GSDMD in fibroblasts incubated with plasma EVs, BEVs, plasma EV-depleted BEVs or BEVs plus a CASPASE-1 inhibitor (Ac-YVAD-cmk, 1 mg/mL) for 24 h. Scale bar, 10 μm.

    Ac-YVAD-cmk purchased from MedChemExpress. Usage Cited in: Bone Res. 2024 Feb 22;12(1):11.  [Abstract]

    Ac-YVAD-cmk (1 μg/rat three times per week) inhibited pyroptosis and prevented NHO development in rats. Schematic and micro-CT images (scale bars, 5 mm) and H&E staining images (scale bars, 100 μm) of heterotopic calcification in the Achilles tendon 3 weeks after surgery.

    Ac-YVAD-cmk purchased from MedChemExpress. Usage Cited in: Bone Res. 2024 Feb 22;12(1):11.  [Abstract]

    Ac-YVAD-cmk (1 μg/rat three times per week). Representative confocal images of Achilles tendons stained with α-SMA (green), N-GSDMD (red) and DAPI (blue) in the rat tendons 3 days after surgery. Scale bars, 100 μm.

    Ac-YVAD-cmk purchased from MedChemExpress. Usage Cited in: Chem Res Toxicol. 2018 Dec 17;31(12):1418-1425.  [Abstract]

    HUVEC cells are stimulated with TCBQ for 6 h, pre-treated with either 10 μM Z-VADFMK (pan-caspase inhibitor) or 10 μM AC-YVAD-CHO (caspase 1/4/5 inhibitor) for 1 h. Then caspase 1/4/5 and GSDMD expressions are detected by Western blotting analysis.

    Caspase アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Ac-YVAD-cmk (Caspase-1 Inhibitor II) is a selective caspase-1 (IL-1beta converting enzyme, ICE)) inhibitor with neuroprotective and anti-inflammatory effects. Ac-YVAD-cmk effectively suppresses the expression of IL-1β and IL-18. Ac-YVAD-cmk inhibits pyroptosis in many diseases[1][2].

    IC50 & Target[1]

    Caspase-1

     

    体外実験

    Ac-YVAD-cmk (40 μM or 80 μM) reduces the expression of IL-1β and IL-18 in activated microglia in vitro[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Ac-YVAD-cmk 関連抗体
    体内実験

    Ac-YVAD-cmk treatment (1 μg/rats; SD rat; injected into the left lateral ventricle) significantly decreases the protein levels of caspase-1 (p20), mature IL-1β/IL-18 compared with the ICH group[2].
    Ac-YVAD-cmk (rats with ac-YVAD-cmk at a dose of 12.5 μmol/kg) significantly reduces mortality from 83 to 33% using Log Rank analysis[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    540.99

    分子式

    C24H33ClN4O8
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    OC1=CC=C(C[C@H](NC(C)=O)C(N[C@H](C(N[C@@H](C)C(N[C@@H](CC(O)=O)C(CCl)=O)=O)=O)C(C)C)=O)C=C1
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    -20°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (184.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.8485 mL 9.2423 mL 18.4846 mL
    5 mM 0.3697 mL 1.8485 mL 3.6969 mL
    10 mM 0.1848 mL 0.9242 mL 1.8485 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (3.84 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.08 mg/mL (3.84 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.63%

    COA (251 KB) HNMR (321 KB) RP-HPLC (230 KB) MS (236 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.8485 mL 9.2423 mL 18.4846 mL 46.2116 mL
    5 mM 0.3697 mL 1.8485 mL 3.6969 mL 9.2423 mL
    10 mM 0.1848 mL 0.9242 mL 1.8485 mL 4.6212 mL
    15 mM 0.1232 mL 0.6162 mL 1.2323 mL 3.0808 mL
    20 mM 0.0924 mL 0.4621 mL 0.9242 mL 2.3106 mL
    25 mM 0.0739 mL 0.3697 mL 0.7394 mL 1.8485 mL
    30 mM 0.0616 mL 0.3081 mL 0.6162 mL 1.5404 mL
    40 mM 0.0462 mL 0.2311 mL 0.4621 mL 1.1553 mL
    50 mM 0.0370 mL 0.1848 mL 0.3697 mL 0.9242 mL
    60 mM 0.0308 mL 0.1540 mL 0.3081 mL 0.7702 mL
    80 mM 0.0231 mL 0.1155 mL 0.2311 mL 0.5776 mL
    100 mM 0.0185 mL 0.0924 mL 0.1848 mL 0.4621 mL
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    Ac-YVAD-cmk Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Ac-YVAD-cmk178603-78-6Caspase-1 Inhibitor IICaspasecaspase-1IL-1βIL-6IL-18intracerebral hemorrhageinflammatoryInhibitorinhibitorinhibit

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    製品名:
    Ac-YVAD-cmk
    製品番号:
    HY-16990
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