Leronlimab  (Synonyms: PRO 140)

Leronlimab (PRO 140) is a humanized IgG4 anti-CCR5 monoclonal antibody. Leronlimab inhibits CCR5-mediated HIV-1 viral and lung metastasis in mouse tumor models. Leronlimab can be used for the research of HIV nonalcoholic steatohepatitis (NASH) and cancer^[1].

Human IgG4 kappa

Human IgG4 (S228P) kappa, Isotype Control

Human

CCR5/CD195

Leronlimab (1-140 mg/mL) binds to CCR5-positive cells up to 98%^[1].
Leronlimab (80-1750 μg/mL) blocks CCL5, CCL3, and CCL4-induced calcium responses in MDA-MB-231-CCR5 cells^[1].
Leronlimab (175 and 350 mg/mL) blocks MDA-MB-231 cell invasion^[1].
Leronlimab (10 μg/mL; 72 hours) enhances the cytotoxicity of doxorubicin for MDA-MB-231 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Leronlimab (2 mg; i.p., twice a week) reduces lung metastasis in murine xenograft models^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1234  [NCBI]

P51681

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

146.7 kDa

674782-26-4

Liquid

Colorless to light yellow

[Leronlimab]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Human IgG4 kappa

• 
  Flow cytometric analysis of 1.5×10⁶ THP-1 cells with Leronlimab (HY-P99697, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG4 (S228P) kappa Isotype Control (HY-P99003, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

• [1]. Jiao X, et al. Leronlimab, a humanized monoclonal antibody to CCR5, blocks breast cancer cellular metastasis and enhances cell death induced by DNA damaging chemotherapy. Breast Cancer Res. 2021 Jan 23;23(1):11.  [Content Brief]