Lycorine

Name: Lycorine
Brand: MedChemExpress
SKU: HY-N0288
Rating: 5.0 (22 reviews)

製品番号: HY-N0288 純度: 99.98%: Data Sheet SDS COA 取扱説明書
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Lycorine is a natural alkaloid extracted from the Amaryllidaceae plant. Lycorine is a potent and orally active SCAP inhibitor with a K_d value 15.24 nM. Lycorine downregulates the SCAP protein level without changing its transcription. Lycorine is also a melanoma vasculogenic inhibitor. Lycorine can be used for the study of prostate cancer and metabolic diseases.

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CAS 番号 : 476-28-8

容量	価格（税別）	在庫状況	数量

>無料サンプル (0.1 - 0.2 mg)		今すぐ申し込む
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 96	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 96	在庫あり	Estimated Time of Arrival: December 31
Solid
50 mg	$88	在庫あり	Estimated Time of Arrival: December 31
100 mg	$143	在庫あり	Estimated Time of Arrival: December 31
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カスタマーレビュー

Based on 22 publication(s) in Google Scholar

Other Forms of Lycorine:

顧客検証

Cell Proliferation/Viability Assay

Cell Migration/Invasion Assay

Flow Cytometry

In Vivo Efficacy Study

: Lycorine purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2022 Mar 30;41(1):116. [Abstract]; Immunoblot analysis of SCAP protein expression at different concentrations. PLC-SR cells (control) were treated with Lycorine (Ly) (1, 2, 4 μM) alone, Sorafenib (Sora) alone or a combination of Lycorine and Sorafenib (Ly + Sora).

: Lycorine purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2022 Mar 30;41(1):116. [Abstract]; The combined use of Lycorine (1, 2, 4 μM) and Sorafenib significantly inhibited the proliferation of PLC-SR cells.

: Lycorine purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2022 Mar 30;41(1):116. [Abstract]; Lycorine (1, 2, 4 μM) combined with Sorafenib decelerated the migration and invasion of sorafenib-resistant HCC cells.

: Lycorine purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2022 Mar 30;41(1):116. [Abstract]; Flow cytometry analysis indicated that when Lycorine (1, 2, 4 μM) was combined with Sorafenib, the percentage of S phase cells was decreased, and apoptosis was significantly increased.

: Lycorine purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2022 Mar 30;41(1):116. [Abstract]; Lycorine (10 mg/kg, i.g.) combined with Sorafenib can reduce the tumor volume in mice.

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製品説明

Lycorine is a natural alkaloid extracted from the Amaryllidaceae plant. Lycorine is a potent and orally active SCAP inhibitor with a K_d value 15.24 nM. Lycorine downregulates the SCAP protein level without changing its transcription^[2]. Lycorine is also a melanoma vasculogenic inhibitor^[3]. Lycorine can be used for the study of prostate cancer and metabolic diseases^[2].

IC₅₀ & Target

Kd: 15.24 nM (SCAP)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A-431	ED₅₀	1.3 μg/mL Compound: 2	Cytotoxicity against human A431 cells after 3 days by sulforhodamine B assay Cytotoxicity against human A431 cells after 3 days by sulforhodamine B assay	[PMID: 8229016]
A2780	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human A2780 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human A2780 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
A549	GI₅₀	0.9 μM Compound: 1	Growth inhibition of pro-apoptotic stimuli-resistant human A549 cells after 72 hrs by MTT assay Growth inhibition of pro-apoptotic stimuli-resistant human A549 cells after 72 hrs by MTT assay	[PMID: 22019045]
A549	GI₅₀	3 μM Compound: 1	Growth inhibition of human A549 cells by MTT assay Growth inhibition of human A549 cells by MTT assay	[PMID: 24393582]
A549	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human A549 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human A549 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
A549	IC₅₀	1.9 μM Compound: 14	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 23190013]
A549	IC₅₀	2 μM Compound: lycorine	Growth inhibition of apoptosis-resistant human A549 cells after 72 hrs by MTT assay Growth inhibition of apoptosis-resistant human A549 cells after 72 hrs by MTT assay	[PMID: 20415482]
A549	IC₅₀	4.2 μM Compound: 1	Cytotoxicity against human A549 cells after 72 hrs by MTT colorimetric assay Cytotoxicity against human A549 cells after 72 hrs by MTT colorimetric assay	[PMID: 20550100]
A549	IC₅₀	4.3 μM Compound: 1, lycorine	Growth inhibition of proapoptotic stimuli-resistant human A549 cells after 72 hrs by MTT assay Growth inhibition of proapoptotic stimuli-resistant human A549 cells after 72 hrs by MTT assay	[PMID: 19788245]
AGS	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human AGS cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human AGS cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
B16-F10	GI₅₀	2 μM Compound: 1	Growth inhibition of pro-apoptotic stimuli-sensitive mouse B16F10 cells after 72 hrs by MTT assay Growth inhibition of pro-apoptotic stimuli-sensitive mouse B16F10 cells after 72 hrs by MTT assay	[PMID: 22019045]
B16-F10	GI₅₀	2.6 μM Compound: 1	Growth inhibition of mouse B16F10 cells by MTT assay Growth inhibition of mouse B16F10 cells by MTT assay	[PMID: 24393582]
B16-F10	IC₅₀	2 μM Compound: lycorine	Growth inhibition of apoptosis-sensitive mouse B16F10 cells after 72 hrs by MTT assay Growth inhibition of apoptosis-sensitive mouse B16F10 cells after 72 hrs by MTT assay	[PMID: 20415482]
B16-F10	IC₅₀	6.3 μM Compound: 1	Cytotoxicity against mouse B16F10 cells after 72 hrs by MTT colorimetric assay Cytotoxicity against mouse B16F10 cells after 72 hrs by MTT colorimetric assay	[PMID: 20550100]
B16-F10	IC₅₀	6.3 μM Compound: 1, lycorine	Growth inhibition of proapoptotic stimuli-sensitive mouse B16F10 cells after 72 hrs by MTT assay Growth inhibition of proapoptotic stimuli-sensitive mouse B16F10 cells after 72 hrs by MTT assay	[PMID: 19788245]
BEAS-2B	IC₅₀	4.9 μM Compound: 14	Cytotoxicity against human BEAS2B cells after 48 hrs by MTT assay Cytotoxicity against human BEAS2B cells after 48 hrs by MTT assay	[PMID: 23190013]
BHK-21	CC₅₀	4.37 μM Compound: 8	cytotoxicity against golden hamster BHK-21 cells incubated for 72 hrs by MTT assay cytotoxicity against golden hamster BHK-21 cells incubated for 72 hrs by MTT assay	[PMID: 32845145]
BJ	IC₅₀	1.9 μM Compound: 2	Cytotoxicity against human BJ cells after 72 hrs by Calcein AM assay Cytotoxicity against human BJ cells after 72 hrs by Calcein AM assay	[PMID: 22921081]
BT-549	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human BT-549 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human BT-549 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
CCRF-CEM	IC₅₀	1.6 μM Compound: 2	Cytotoxicity against human CEM cells after 72 hrs by Calcein AM assay Cytotoxicity against human CEM cells after 72 hrs by Calcein AM assay	[PMID: 22921081]
COLO 201	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human COLO201 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human COLO201 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
G-361	IC₅₀	5 μM Compound: 2	Cytotoxicity against human G361 cells after 72 hrs by Calcein AM assay Cytotoxicity against human G361 cells after 72 hrs by Calcein AM assay	[PMID: 22921081]
HEK-293T	CC₅₀	1.044 μM Compound: 95	Cytotoxicity against human HEK293T cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Cytotoxicity against human HEK293T cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 37597436]
HL-60	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human HL-60 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human HL-60 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
HL-60	IC₅₀	1.6 μM Compound: 14	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 23190013]
HT-1080	ED₅₀	0.5 μg/mL Compound: 2	Cytotoxicity against human HT1080 cells after 3 days by sulforhodamine B assay Cytotoxicity against human HT1080 cells after 3 days by sulforhodamine B assay	[PMID: 8229016]
HT-29	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human HT-29 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human HT-29 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
HT-29	IC₅₀	3.2 μM Compound: 14	Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay	[PMID: 23190013]
HeLa	IC₅₀	10.6 μM Compound: 2	Cytotoxicity against human HeLa cells after 72 hrs by Calcein AM assay Cytotoxicity against human HeLa cells after 72 hrs by Calcein AM assay	[PMID: 22921081]
HepG2	IC₅₀	3.7 μM Compound: 14	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 23190013]
Hs 683	GI₅₀	0.9 μM Compound: 1	Growth inhibition of pro-apoptotic stimuli-sensitive human Hs683 cells after 72 hrs by MTT assay Growth inhibition of pro-apoptotic stimuli-sensitive human Hs683 cells after 72 hrs by MTT assay	[PMID: 22019045]
Hs 683	GI₅₀	2.1 μM Compound: 1	Growth inhibition of human Hs683 cells by MTT assay Growth inhibition of human Hs683 cells by MTT assay	[PMID: 24393582]
Hs 683	IC₅₀	2 μM Compound: lycorine	Growth inhibition of apoptosis-sensitive human Hs 683 cells after 72 hrs by MTT assay Growth inhibition of apoptosis-sensitive human Hs 683 cells after 72 hrs by MTT assay	[PMID: 20415482]
Hs 683	IC₅₀	6.7 μM Compound: 1, lycorine	Growth inhibition of proapoptotic stimuli-sensitive human Hs683 cells after 72 hrs by MTT assay Growth inhibition of proapoptotic stimuli-sensitive human Hs683 cells after 72 hrs by MTT assay	[PMID: 19788245]
Hs 683	IC₅₀	6.9 μM Compound: 1	Cytotoxicity against human Hs683 cells after 72 hrs by MTT colorimetric assay Cytotoxicity against human Hs683 cells after 72 hrs by MTT colorimetric assay	[PMID: 20550100]
Huh-7	CC₅₀	0.834 μM Compound: 95	Cytotoxicity against human Huh-7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human Huh-7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 37597436]
Huh-7	CC₅₀	66.12 μM Compound: GNF-Pf-4974	NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7) NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)	[PMID: 18579783]
Jurkat	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human Jurkat cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human Jurkat cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
K562	IC₅₀	2.3 μM Compound: 14	Cytotoxicity against human K562 cells after 48 hrs by MTT assay Cytotoxicity against human K562 cells after 48 hrs by MTT assay	[PMID: 23190013]
K562	IC₅₀	3.6 μM Compound: 2	Cytotoxicity against human K562 cells after 72 hrs by Calcein AM assay Cytotoxicity against human K562 cells after 72 hrs by Calcein AM assay	[PMID: 22921081]
KB	ED₅₀	0.3 μg/mL Compound: 2	Cytotoxicity against human KB cells after 3 days by sulforhodamine B assay Cytotoxicity against human KB cells after 3 days by sulforhodamine B assay	[PMID: 8229016]
KB-V1	ED₅₀	0.4 μg/mL Compound: 2	Cytotoxicity against human KBV1 cells after 3 days by sulforhodamine B assay Cytotoxicity against human KBV1 cells after 3 days by sulforhodamine B assay	[PMID: 8229016]
LLC-MK2	CC₅₀	3.81 μM Compound: 8	Cytotoxicity against Rhesus macaque LLC-MK2 cells incubated for 72 hrs by MTT assay Cytotoxicity against Rhesus macaque LLC-MK2 cells incubated for 72 hrs by MTT assay	[PMID: 32845145]
LNCaP	ED₅₀	0.5 μg/mL Compound: 2	Cytotoxicity against human LNCAP cells after 3 days by sulforhodamine B assay Cytotoxicity against human LNCAP cells after 3 days by sulforhodamine B assay	[PMID: 8229016]
MCF7	GI₅₀	10.9 μM Compound: 1	Growth inhibition of human MCF7 cells by MTT assay Growth inhibition of human MCF7 cells by MTT assay	[PMID: 24393582]
MCF7	GI₅₀	4 μM Compound: 1	Growth inhibition of human MCF7 cells after 72 hrs by MTT assay Growth inhibition of human MCF7 cells after 72 hrs by MTT assay	[PMID: 22019045]
MCF7	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human MCF7 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human MCF7 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
MCF7	IC₅₀	13 μM Compound: 2	Cytotoxicity against human MCF7 cells after 72 hrs by Calcein AM assay Cytotoxicity against human MCF7 cells after 72 hrs by Calcein AM assay	[PMID: 22921081]
MDA-MB-231	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human MDA-MB-231 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human MDA-MB-231 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
MDCK	CC₅₀	20.9 μM Compound: 24; AA1	Cytotoxicity against MDCK cells incubated for 72 hrs by CCK-8 assay Cytotoxicity against MDCK cells incubated for 72 hrs by CCK-8 assay	[PMID: 38283223]
MOLT-4	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human MOLT-4 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human MOLT-4 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
Macrophage	IC₅₀	2.5 μM Compound: 9	Inhibition of LPS-induced NO production in ddY mouse macrophages after 20 hrs by Griess reagent assay Inhibition of LPS-induced NO production in ddY mouse macrophages after 20 hrs by Griess reagent assay	[PMID: 15270564]
NCI-H1299	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human NCI-H1299 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human NCI-H1299 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
NHDF	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human NHDF cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human NHDF cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
NHDF	IC₅₀	> 100 μM Compound: 1, lycorine	Growth inhibition of human NHDF after 72 hrs by MTT assay Growth inhibition of human NHDF after 72 hrs by MTT assay	[PMID: 19788245]
P388	ED₅₀	0.9 μg/mL Compound: 2	Cytotoxicity against mouse P388 cells after 3 days by sulforhodamine B assay Cytotoxicity against mouse P388 cells after 3 days by sulforhodamine B assay	[PMID: 8229016]
PANC-1	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human PANC-1 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human PANC-1 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
PC-3	GI₅₀	4 μM Compound: 1	Growth inhibition of human PC3 cells after 72 hrs by MTT assay Growth inhibition of human PC3 cells after 72 hrs by MTT assay	[PMID: 22019045]
SAOS-2	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human SAOS-2 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human SAOS-2 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
SK-BR-3	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human SK-BR-3 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human SK-BR-3 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
SK-MEL	IC₅₀	8.4 μM Compound: 1	Cytotoxicity against human SK-MEL cells after 72 hrs by MTT colorimetric assay Cytotoxicity against human SK-MEL cells after 72 hrs by MTT colorimetric assay	[PMID: 20550100]
SK-MEL-2	ED₅₀	1.6 μg/mL Compound: 2	Cytotoxicity against human SK-MEL-2 cells after 3 days by sulforhodamine B assay Cytotoxicity against human SK-MEL-2 cells after 3 days by sulforhodamine B assay	[PMID: 8229016]
SK-MEL-28	GI₅₀	3.7 μM Compound: 1	Growth inhibition of human SK-MEL-28 cells by MTT assay Growth inhibition of human SK-MEL-28 cells by MTT assay	[PMID: 24393582]
SK-MEL-28	GI₅₀	4 μM Compound: 1	Growth inhibition of pro-apoptotic stimuli-resistant human SK-MEL-28 cells after 72 hrs by MTT assay Growth inhibition of pro-apoptotic stimuli-resistant human SK-MEL-28 cells after 72 hrs by MTT assay	[PMID: 22019045]
SK-MEL-28	IC₅₀	2 μM Compound: lycorine	Growth inhibition of apoptosis-resistant human SK-MEL-28 cells after 72 hrs by MTT assay Growth inhibition of apoptosis-resistant human SK-MEL-28 cells after 72 hrs by MTT assay	[PMID: 20415482]
SK-MEL-28	IC₅₀	8.5 μM Compound: 1, lycorine	Growth inhibition of proapoptotic stimuli-resistant human SK-MEL-28 cells after 72 hrs by MTT assay Growth inhibition of proapoptotic stimuli-resistant human SK-MEL-28 cells after 72 hrs by MTT assay	[PMID: 19788245]
SW480	IC₅₀	0.3 μM Compound: Lycorine	Cytotoxicity against human SW480 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay Cytotoxicity against human SW480 cells assessed as cell growth inhibition incubated for 48 hrs by WST-1 assay	[PMID: 34536668]
T98G	GI₅₀	3 μM Compound: 1	Growth inhibition of pro-apoptotic stimuli-resistant human T98G cells after 72 hrs by MTT assay Growth inhibition of pro-apoptotic stimuli-resistant human T98G cells after 72 hrs by MTT assay	[PMID: 22019045]
T98G	GI₅₀	5.2 μM Compound: 1	Growth inhibition of human T98G cells by MTT assay Growth inhibition of human T98G cells by MTT assay	[PMID: 24393582]
U-373MG ATCC	ED₅₀	0.3 μg/mL Compound: 2	Cytotoxicity against human U373 cells after 3 days by sulforhodamine B assay Cytotoxicity against human U373 cells after 3 days by sulforhodamine B assay	[PMID: 8229016]
U-373MG ATCC	GI₅₀	3 μM Compound: 1	Growth inhibition of pro-apoptotic stimuli-resistant human U373 cells after 72 hrs by MTT assay Growth inhibition of pro-apoptotic stimuli-resistant human U373 cells after 72 hrs by MTT assay	[PMID: 22019045]
U-373MG ATCC	IC₅₀	2 μM Compound: lycorine	Growth inhibition of apoptosis-resistant human U373 cells after 72 hrs by MTT assay Growth inhibition of apoptosis-resistant human U373 cells after 72 hrs by MTT assay	[PMID: 20415482]
U-373MG ATCC	IC₅₀	7.6 μM Compound: 1	Cytotoxicity against human U373 cells after 72 hrs by MTT colorimetric assay Cytotoxicity against human U373 cells after 72 hrs by MTT colorimetric assay	[PMID: 20550100]
U-373MG ATCC	IC₅₀	7.6 μM Compound: 1, lycorine	Growth inhibition of proapoptotic stimuli-resistant human U373 cells after 72 hrs by MTT assay Growth inhibition of proapoptotic stimuli-resistant human U373 cells after 72 hrs by MTT assay	[PMID: 19788245]
Vero	CC₅₀	21 μM Compound: 14	Cytotoxicity against African green monkey Vero cells Cytotoxicity against African green monkey Vero cells	[PMID: 34274831]
Vero	CC₅₀	> 1000 μM Compound: 95	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability measured after 72 hrs by MTS assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability measured after 72 hrs by MTS assay	[PMID: 37597436]
Vero	IC₅₀	0.24 μg/mL Compound: 16	Antiviral activity against Dengue virus type 4 infected in african green monkey Vero cells after 6 days by plaque reduction assay Antiviral activity against Dengue virus type 4 infected in african green monkey Vero cells after 6 days by plaque reduction assay	[PMID: 1336040]
Vero	IC₅₀	0.28 μg/mL Compound: 16	Antiviral activity against Yellow fever virus Ashibi infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect by MTT assay Antiviral activity against Yellow fever virus Ashibi infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect by MTT assay	[PMID: 1336040]
Vero	IC₅₀	0.33 μg/mL Compound: 16	Antiviral activity against Japanese encephalitis virus Nakayama infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect by MTT assay Antiviral activity against Japanese encephalitis virus Nakayama infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect by MTT assay	[PMID: 1336040]
Vero	IC₅₀	0.5 μg/mL Compound: 16	Antiviral activity against Punta Toro virus Adames infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect by MTT assay Antiviral activity against Punta Toro virus Adames infected in african green monkey Vero cells assessed as inhibition of virus-induced cytopathic effect by MTT assay	[PMID: 1336040]
Vero	IC₅₀	0.93 μg/mL Compound: 16	Antiviral activity against Rift Valley fever virus infected in african green monkey Vero cells after 6 days by plaque reduction assay Antiviral activity against Rift Valley fever virus infected in african green monkey Vero cells after 6 days by plaque reduction assay	[PMID: 1336040]
Vero C1008	CC₅₀	14.9 μM Compound: 8	Cytotoxicity against African green monkey Vero E6 cells incubated for 72 hrs by MTS assay Cytotoxicity against African green monkey Vero E6 cells incubated for 72 hrs by MTS assay	[PMID: 32845145]
WI-38	IC₅₀	> 100 μM Compound: 1, lycorine	Growth inhibition of human WI38 cells after 72 hrs by MTT assay Growth inhibition of human WI38 cells after 72 hrs by MTT assay	[PMID: 19788245]
ZR-75-1	ED₅₀	0.9 μg/mL Compound: 2	Cytotoxicity against human ZR-75-1 cells after 3 days by sulforhodamine B assay Cytotoxicity against human ZR-75-1 cells after 3 days by sulforhodamine B assay	[PMID: 8229016]

体外実験

Lycorine inhibits cell proliferation in a dose-dependent manner in the abovementioned 4 PCa cell lines, and the IC₅₀ ranged from 5 μM to 10 μM., it also shows Lycorine has little effects on PNT1A cell's proliferation^[1].
SCAP (SREBF chaperone) is an ER-toGolgi transport protein, undergoes a conformational change, and regulates/preserves SREBFs in the ER by forming a complex with INSIG1 (insulin induced gene 1)^[2].
Lycorine (5-40 μM; 16 hours) significantly suppresses SREBFs activity (up to -70%) in a dosedependent manner and does not cause obvious cytotoxicity in cells^[2].
Lycorine (10-20 μM; 2-16 hours) dose- and time-dependently decreases the mature SREBF1 and SREBF2 proteins in HL-7702 cells^[2].
Lycorine (20 μM; 16 hours) neither activates NR1H3 transcription nor affect NR1H3 target genes, including ABCG5? and ABCG8, but Sterols activates NR1H3 transcription activity^[1].
Lycorine (0-25 μM; 48 hours) treatment markedly suppresses the expression of vascular endothelial (VE)-cadherin in a dose-dependent manner and also slightly diminishes the expression of Sema4D in C8161 cells. However, the expression of the other six genes is not affected^[3].
Lycorine (0-25 μM; 48 hours) treatment markedly reduces VE-cadherin protein levels in C8161 cells in a dose-dependent fashion^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	HL-7702/SRE-Luc cells
Concentration:	16 hours
Incubation Time:	5 μM; 10 μM; 20 μM; 40 μM
Result:	Had no cytotoxicity on HL-7702 cells.

Western Blot Analysis^[2]

Cell Line:	HL-7702/SRE-Luc cells
Concentration:	2 hours, 4 hours, 8 hours, 12 hours, 16 hours
Incubation Time:	10 μM; 20 μM
Result:	Decreased p-SREBF1, m-SREBF1, p-SREBF2 and p-SREBF1 protein expression.

RT-PCR^[3]

Cell Line:	C8161 cells
Concentration:	0 μM, 1.56 μM, 3.13 μM, 6.25 μM, 12.5 μM, 25 μM
Incubation Time:	48 hours
Result:	Markedly suppressed the expression of VE-cadherin in a dose-dependent manner and also slightly diminished the expression of Sema4D in C8161 cells.

体内実験

Lycorine (oral chow; 15 mg/kg, 30 mg/kg; once daily) alleviates fat accumulation and metabolic syndrome, increases lipolysis and betaoxidation of fatty acid along with precursor and mature SREBFs in mice ^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	High-fat diet (HFD)-fed C57BL/6J mice^[2]
Dosage:	15 mg/kg, 30 mg/kg
Administration:	Oral chow; once daily
Result:	Ameliorated high-fat diet-induced hyperlipidemia, hepatic steatosis, and insulin resistance in mice.

分子量

287.31

分子式

C₁₆H₁₇NO₄

CAS 番号

476-28-8

Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]1[C@@H](O)C=C(CC2)[C@@]3([H])N2CC4=C([C@@]31[H])C=C(OCO5)C5=C4

Structure Classification

Initial Source

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 25 mg/mL (87.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4806 mL	17.4028 mL	34.8056 mL
5 mM	0.6961 mL	3.4806 mL	6.9611 mL
10 mM	0.3481 mL	1.7403 mL	3.4806 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 25 mg/mL (87.01 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.98%

Select Batch:

データシート (286 KB) SDS (480 KB)

COA (257 KB) HNMR (254 KB) RP-HPLC (246 KB) MS (72 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Hu M, et al.Lycorine is a novel inhibitor of the growth and metastasis of hormone-refractory prostate cancer.Oncotarget. 2015 Jun 20;6(17):15348-61.

[2]. Zu-Guo Zheng, et al. Discovery of a Potent SCAP Degrader That Ameliorates HFD-induced Obesity, Hyperlipidemia and Insulin Resistance via an Autophagy-Independent Lysosomal Pathway. Autophagy. 2020 May 20;1-22. [Content Brief]

[3]. [1].Liu, R. et al. Lycorine hydrochloride inhibits metastatic melanoma cell-dominant vasculogenic mimicry. Pigment cell & melanoma research 25, 630-638, doi:10.1111/j.1755-148X.2012.01036.x (2012). [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.4806 mL	17.4028 mL	34.8056 mL	87.0140 mL
	5 mM	0.6961 mL	3.4806 mL	6.9611 mL	17.4028 mL
	10 mM	0.3481 mL	1.7403 mL	3.4806 mL	8.7014 mL
	15 mM	0.2320 mL	1.1602 mL	2.3204 mL	5.8009 mL
	20 mM	0.1740 mL	0.8701 mL	1.7403 mL	4.3507 mL
	25 mM	0.1392 mL	0.6961 mL	1.3922 mL	3.4806 mL
	30 mM	0.1160 mL	0.5801 mL	1.1602 mL	2.9005 mL
	40 mM	0.0870 mL	0.4351 mL	0.8701 mL	2.1754 mL
	50 mM	0.0696 mL	0.3481 mL	0.6961 mL	1.7403 mL
	60 mM	0.0580 mL	0.2900 mL	0.5801 mL	1.4502 mL
	80 mM	0.0435 mL	0.2175 mL	0.4351 mL	1.0877 mL

Lycorine Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量		濃度		体積		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）	×	体積 _（開始）	=	濃度 _（終了）	×	体積 _（終了）
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Lycorine

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Other Forms of Lycorine:

Lycorine 関連抗体

顧客検証

おすすめ

•関連スクリーニングライブラリ:

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生物活性

純度とドキュメンテーション

参考文献

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Lycorine 関連抗体

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Lycorine Related Classifications

Molarity Calculator

Dilution Calculator

The molarity calculator equation

The dilution calculator equation

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