Lycorine hydrochloride

Name: Lycorine hydrochloride
Brand: MedChemExpress
SKU: HY-N0289
Rating: 5.0 (12 reviews)

Cat. No.: HY-N0289 Purity: 98.38%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Lycorine hydrochloride is the main active ingredient of the herbal medicine derived from Lycoris radiata (L’Her.) Herb. and is also a melanoma vasculogenic inhibitor and has anti-tumor activity. Lycorine hydrochloride effectively inhibits mitotic proliferation of Hey1B cells (IC₅₀ of 1.2 μM).

For research use only. We do not sell to patients.

Lycorine hydrochloride Chemical Structure

CAS No. : 2188-68-3

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
25 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 12 publication(s) in Google Scholar

Other Forms of Lycorine hydrochloride:

Lycorine In-stock

12 Publications Citing Use of MCE Lycorine hydrochloride

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Lycorine hydrochloride is the main active ingredient of the herbal medicine derived from Lycoris radiata (L’Her.) Herb. and is also a melanoma vasculogenic inhibitor and has anti-tumor activity^[1]. Lycorine hydrochloride effectively inhibits mitotic proliferation of Hey1B cells (IC₅₀ of 1.2 μM)^[2].

IC₅₀ & Target

IC50: 1.2 μM (Hey1B cells )^[2]

In Vitro

Lycorine hydrochloride (0-25 μM; 48 hours; C8161 cells) treatment markedly suppresses the expression of vascular endothelial (VE)-cadherin in a dose-dependent manner and also slightly diminishes the expression of Sema4D in C8161 cells. However, the expression of the other six genes is not affected^[1].
Lycorine hydrochloride (0-25 μM; 48 hours; C8161 cells) treatment markedly reduces VE-cadherin protein levels in C8161 cells in a dose-dependent fashion^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:	C8161 cells
Concentration:	0 μM, 1.56 μM, 3.13 μM, 6.25 μM, 12.5 μM, 25 μM
Incubation Time:	48 hours
Result:	Markedly suppressed the expression of VE-cadherin in a dose-dependent manner and also slightly diminished the expression of Sema4D in C8161 cells.

Western Blot Analysis^[1]

Cell Line:	C8161 cells
Concentration:	0 μM, 1.56 μM, 3.13 μM, 6.25 μM, 12.5 μM, 25 μM
Incubation Time:	48 hours
Result:	Markedly reduced VE-cadherin protein levels in C8161 cells in a dose-dependent fashion.

In Vivo

Lycorine hydrochloride (0-15 μg; subcutaneous injection; for 14 days; BALB/C nude mice) treatment has an inhibitory effect on melanoma cell-mediated tumor vasculogenic mimicry^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	6- to 8-week-old BALB/C nude mice^[1]
Dosage:	0 μg, 7.5 μg, 15 μg
Administration:	Subcutaneous injection; for 14 days
Result:	Showed an inhibitory effect on melanoma cell-mediated tumor vasculogenic mimicry.

Molecular Weight

323.77

Formula

C₁₆H₁₈ClNO₄

CAS No.

2188-68-3

Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]1[C@@H](O)C=C(CC2)[C@@]3([H])N2CC4=C([C@@]31[H])C=C(OCO5)C5=C4.Cl

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (77.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 16.67 mg/mL (51.49 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0886 mL	15.4431 mL	30.8861 mL
5 mM	0.6177 mL	3.0886 mL	6.1772 mL
10 mM	0.3089 mL	1.5443 mL	3.0886 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (6.42 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.89%

Select Batch:

Data Sheet (285 KB) SDS (420 KB)

COA (259 KB) HNMR (354 KB) LCMS (138 KB)

Handling Instructions (2659 KB)

References

[1]. Liu, R. et al. Lycorine hydrochloride inhibits metastatic melanoma cell-dominant vasculogenic mimicry. Pigment cell & melanoma research 25, 630-638, doi:10.1111/j.1755-148X.2012.01036.x (2012). [Content Brief]

[2]. Cao, Z. et al.Lycorine hydrochloride selectively inhibits human ovarian cancer cell proliferation and tumor neovascularization with very low toxicity. Toxicology letters 218, 174-185, doi:10.1016/j.toxlet.2013.01.018 (2013). [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.0886 mL	15.4431 mL	30.8861 mL	77.2153 mL
	5 mM	0.6177 mL	3.0886 mL	6.1772 mL	15.4431 mL
	10 mM	0.3089 mL	1.5443 mL	3.0886 mL	7.7215 mL
	15 mM	0.2059 mL	1.0295 mL	2.0591 mL	5.1477 mL
	20 mM	0.1544 mL	0.7722 mL	1.5443 mL	3.8608 mL
	25 mM	0.1235 mL	0.6177 mL	1.2354 mL	3.0886 mL
	30 mM	0.1030 mL	0.5148 mL	1.0295 mL	2.5738 mL
	40 mM	0.0772 mL	0.3861 mL	0.7722 mL	1.9304 mL
	50 mM	0.0618 mL	0.3089 mL	0.6177 mL	1.5443 mL
DMSO	60 mM	0.0515 mL	0.2574 mL	0.5148 mL	1.2869 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.