1. Metabolic Enzyme/Protease
    Anti-infection
    Apoptosis
  2. Fatty Acid Synthase (FASN)
    Virus Protease
    Bacterial
    Apoptosis
  3. Lycorine

Lycorine 

Cat. No.: HY-N0288 Purity: ≥98.0%
Handling Instructions

Lycorine is a natural alkaloid extracted from the Amaryllidaceae plant. Lycorine is a potent and orally active SCAP inhibitor with a Kd value 15.24 nM. Lycorine downregulates the SCAP protein level without changing its transcription. Lycorine is also a melanoma vasculogenic inhibitor. Lycorine can be used for the study of prostate cancer and metabolic diseases.

For research use only. We do not sell to patients.

Lycorine Chemical Structure

Lycorine Chemical Structure

CAS No. : 476-28-8

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Based on 1 publication(s) in Google Scholar

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Description

Lycorine is a natural alkaloid extracted from the Amaryllidaceae plant. Lycorine is a potent and orally active SCAP inhibitor with a Kd value 15.24 nM. Lycorine downregulates the SCAP protein level without changing its transcription[2]. Lycorine is also a melanoma vasculogenic inhibitor[3]. Lycorine can be used for the study of prostate cancer and metabolic diseases[2].

IC50 & Target

Kd: 15.24 nM (SCAP)[1]

In Vitro

Lycorine inhibits cell proliferation in a dose-dependent manner in the abovementioned 4 PCa cell lines, and the IC50 ranged from 5 μM to 10 μM., it also shows Lycorine has little effects on PNT1A cell's proliferation[1].
SCAP (SREBF chaperone) is an ER-toGolgi transport protein, undergoes a conformational change, and regulates/preserves SREBFs in the ER by forming a complex with INSIG1 (insulin induced gene 1)[2].
Lycorine (5-40 μM; 16 hours) significantly suppresses SREBFs activity (up to -70%) in a dosedependent manner and does not cause obvious cytotoxicity in cells[2].
Lycorine (10-20 μM; 2-16 hours) dose- and time-dependently decreases the mature SREBF1 and SREBF2 proteins in HL-7702 cells[2].
Lycorine (20 μM; 16 hours) neither activates NR1H3 transcription nor affect NR1H3 target genes, including ABCG5 and ABCG8, but Sterols activates NR1H3 transcription activity[1].
Lycorine hydrochloride (0-25 μM; 48 hours) treatment markedly suppresses the expression of vascular endothelial (VE)-cadherin in a dose-dependent manner and also slightly diminishes the expression of Sema4D in C8161 cells. However, the expression of the other six genes is not affected[3].
Lycorine hydrochloride (0-25 μM; 48 hours) treatment markedly reduces VE-cadherin protein levels in C8161 cells in a dose-dependent fashion[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: HL-7702/SRE-Luc cells
Concentration: 16 hours
Incubation Time: 5 μM; 10 μM; 20 μM; 40 μM
Result: Had no cytotoxicity on HL-7702 cells.

Western Blot Analysis[2]

Cell Line: HL-7702/SRE-Luc cells
Concentration: 2 hours, 4 hours, 8 hours, 12 hours, 16 hours
Incubation Time: 10 μM; 20 μM
Result: Decreased p-SREBF1, m-SREBF1, p-SREBF2 and p-SREBF1 protein expression.

RT-PCR[3]

Cell Line: C8161 cells
Concentration: 0 μM, 1.56 μM, 3.13 μM, 6.25 μM, 12.5 μM, 25 μM
Incubation Time: 48 hours
Result: Markedly suppressed the expression of VE-cadherin in a dose-dependent manner and also slightly diminished the expression of Sema4D in C8161 cells.
In Vivo

Lycorine (oral chow; 15 mg/kg, 30 mg/kg; once daily) alleviates fat accumulation and metabolic syndrome, increases lipolysis and betaoxidation of fatty acid along with precursor and mature SREBFs in mice [2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: High-fat diet (HFD)-fed C57BL/6J mice[2]
Dosage: 15 mg/kg, 30 mg/kg
Administration: Oral chow; once daily
Result: Ameliorated high-fat diet-induced hyperlipidemia, hepatic steatosis, and insulin resistance in mice.
Molecular Weight

287.31

Formula

C₁₆H₁₇NO₄

CAS No.

476-28-8

SMILES

O[[email protected]@H]1[[email protected]@H](O)C=C(CC2)[[email protected]@]3([H])N2CC4=C([[email protected]@]31[H])C=C(OCO5)C5=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (87.01 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4806 mL 17.4028 mL 34.8056 mL
5 mM 0.6961 mL 3.4806 mL 6.9611 mL
10 mM 0.3481 mL 1.7403 mL 3.4806 mL
*Please refer to the solubility information to select the appropriate solvent.
References
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Keywords:

LycorineFatty Acid Synthase (FASN)Virus ProteaseBacterialApoptosisanti-prostatecancerPCaSCAPSREBF chaperoneHL-7702ER stressABCG5ABCG8Amaryllidaceaedyslipidemiafatty liverHFDprostatetumormetabolicantiviralantimalarialantiinflammationInhibitorinhibitorinhibit

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