Meclofenamic acid (Synonyms: Meclofenamate)

Name: Meclofenamic acid
Brand: MedChemExpress
SKU: HY-117275
Rating: 5.0 (8 reviews)

Cat. No.: HY-117275 Pureza: 99.66%: Ficha de datos
COA

SDS
Instrucciones de manejo
FAQs
Technical Support

Meclofenamic acid (Meclofenamate) is a non-steroidal anti-inflammatory agent. Meclofenamic acid is a highly selective FTO (fat mass and obesity-associated) enzyme inhibitor. Meclofenamic acid competes with FTO binding for the m(6)A-containing nucleic acid. Meclofenamic acid is a non-selective gap-junction blocker. Meclofenamic acid inhibits hKv2.1 and hKv1.1, with IC₅₀ values of 56.0 and 155.9 μM, respectively.

Para uso exclusivo en investigación. No vendemos a pacientes.

No. CAS : 644-62-2

Tamaño	Stock	Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
1 mg	En stock	Estimated Time of Arrival: December 31
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtener un presupuesto
500 mg	Obtener un presupuesto

or Consulta para venta a granel

* Seleccione Cantidad antes de agregar artículos.

This product is a controlled substance and not for sale in your territory.

Revisión del cliente

Based on 8 publication(s) in Google Scholar

Other Forms of Meclofenamic acid:

Meclofenamic acid sodium In-stock
Meclofenamic acid-d₄ Obtener un presupuesto
Meclofenamic acid-¹³C₆ Obtener un presupuesto
Meclofenamic acid sodium hydrate Obtener un presupuesto
Meclofenamic acid (Standard) Obtener un presupuesto

8 Publications Citing Use of MCE Meclofenamic acid

: Meclofenamic acid purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Jul 25;11(17):8464-8479. [Abstract]; The effect of FTO inhibitor Meclofenamate sodium (MS) (80 μM and 120 μM) on ATF4 protein expression and mTORC1 activity during 968 treatment is detected by western blotting. MS efficiently reduces ATF4 expression and rescues the mTOR downregulation induced by compound 968.

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas Endogenous Metabolite:

Ver todas las isoformas

Human Endogenous Metabolite Microbial Metabolite

Actividad biológica
Pureza y Documentación
Referencias
Revisión del cliente

Descripciòn

IC₅₀ & Target

IC50: 1 μM (cyclooxygenase), 56.0 μM (hKv2.1), 155.9 μM (hKv1.1)^[3]

Cellular Effect

Cell Line	Type	Value	Description	References
RBL-1	IC₅₀	0.1 3 Compound: 1a (Meclofenamic acid)	Compound was evaluated in an intact RBL-1 cell line for inhibition of 5-Cyclooxygenase Compound was evaluated in an intact RBL-1 cell line for inhibition of 5-Cyclooxygenase	10.1016/S0960-894X(00)80657-5
RBL-1	IC₅₀	24 3 Compound: 1a (Meclofenamic acid)	Compound was evaluated in an intact RBL-1 cell line for inhibition of 5-lipoxygenase Compound was evaluated in an intact RBL-1 cell line for inhibition of 5-lipoxygenase	10.1016/S0960-894X(00)80657-5
RBL-1	IC₅₀	0.1 3 Compound: 1a	Inhibition of Prostaglandin G/H synthase mediated PGF2-alpha formation in rat basophilic leukemia (RBL-1) cells Inhibition of Prostaglandin G/H synthase mediated PGF2-alpha formation in rat basophilic leukemia (RBL-1) cells	[PMID: 8515419]
RBL-1	IC₅₀	24 3 Compound: 1a	Inhibition 5-lipoxygenase mediated LTB4 formation in rat basophilic leukemia (RBL-1) cells Inhibition 5-lipoxygenase mediated LTB4 formation in rat basophilic leukemia (RBL-1) cells	[PMID: 8515419]
RBL-1	IC₅₀	0.1 3 Compound: 1a	Inhibition of Prostaglandin G/H synthase mediated PGF2-alpha formation in rat basophilic leukemia (RBL-1) cells Inhibition of Prostaglandin G/H synthase mediated PGF2-alpha formation in rat basophilic leukemia (RBL-1) cells	[PMID: 8515419]
RBL-1	IC₅₀	0.1 3 Compound: 1a (Meclofenamic acid)	Compound was evaluated in an intact RBL-1 cell line for inhibition of 5-Cyclooxygenase Compound was evaluated in an intact RBL-1 cell line for inhibition of 5-Cyclooxygenase	10.1016/S0960-894X(00)80657-5
RBL-1	IC₅₀	24 3 Compound: 1a	Inhibition 5-lipoxygenase mediated LTB4 formation in rat basophilic leukemia (RBL-1) cells Inhibition 5-lipoxygenase mediated LTB4 formation in rat basophilic leukemia (RBL-1) cells	[PMID: 8515419]
RBL-1	IC₅₀	24 3 Compound: 1a (Meclofenamic acid)	Compound was evaluated in an intact RBL-1 cell line for inhibition of 5-lipoxygenase Compound was evaluated in an intact RBL-1 cell line for inhibition of 5-lipoxygenase	10.1016/S0960-894X(00)80657-5

In Vitro

Meclofenamic acid (0-100 μM, 24 h) inhibits FTO demethylation in a dose-response manner^[1].
Meclofenamic acid inhibits enzyme cyclooxygenase, with an IC₅₀ about 1 μM, thereby inhibiting the production of prostaglandins^[2].
Meclofenamic acid inhibits the release of 5-HETE and LTB4 from human neutrophils stimulated with calcium ionophore and antagonizes the response of tissues to certain prostaglandins^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	HeLa cells
Concentration:	0, 12.5, 25, 50, 100 μM
Incubation Time:	24 h
Result:	Inhibited FTO demethylation in a dose-response manner, and elevates the levels of cellular m6A in mRNA by targeting FTO.

Ensayo clínico

Peso molecular

296.15

Fòrmula

C₁₄H₁₁Cl₂NO₂

No. CAS

644-62-2

Appearance

Solid

Color

White to yellow

SMILES

O=C(O)C1=CC=CC=C1NC2=C(Cl)C=CC(C)=C2Cl

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (337.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Methanol : 7.14 mg/mL (24.11 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3767 mL	16.8833 mL	33.7667 mL
5 mM	0.6753 mL	3.3767 mL	6.7533 mL
10 mM	0.3377 mL	1.6883 mL	3.3767 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: 99.66%

Select Batch:

Ficha de datos (278 KB) SDS (480 KB)

COA (251 KB) HNMR (251 KB) LCMS (95 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Huang Y, et al. Meclofenamic acid selectively inhibits FTO demethylation of m6A over ALKBH5. Nucleic Acids Res. 2015 Jan;43(1):373-84. [Content Brief]

[2]. Conroy MC, et al. Pharmacology, pharmacokinetics, and therapeutic use of meclofenamate sodium. Clin J Pain. 1991;7 Suppl 1:S44-8. [Content Brief]

[3]. Lee YT, et al. Inhibition of hKv2.1, a major human neuronal voltage-gated K+ channel, by meclofenamic acid. Eur J Pharmacol. 1999 Aug 13;378(3):349-56. [Content Brief]

[4]. Eleftheriou CG, et al. Meclofenamic acid improves the signal to noise ratio for visual responses produced by ectopicexpression of human rod opsin. Mol Vis. 2017 Jun 16;23:334-345. eCollection 2017. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Methanol / DMSO	1 mM	3.3767 mL	16.8833 mL	33.7667 mL	84.4167 mL
	5 mM	0.6753 mL	3.3767 mL	6.7533 mL	16.8833 mL
	10 mM	0.3377 mL	1.6883 mL	3.3767 mL	8.4417 mL
	15 mM	0.2251 mL	1.1256 mL	2.2511 mL	5.6278 mL
	20 mM	0.1688 mL	0.8442 mL	1.6883 mL	4.2208 mL
DMSO	25 mM	0.1351 mL	0.6753 mL	1.3507 mL	3.3767 mL
	30 mM	0.1126 mL	0.5628 mL	1.1256 mL	2.8139 mL
	40 mM	0.0844 mL	0.4221 mL	0.8442 mL	2.1104 mL
	50 mM	0.0675 mL	0.3377 mL	0.6753 mL	1.6883 mL
	60 mM	0.0563 mL	0.2814 mL	0.5628 mL	1.4069 mL
	80 mM	0.0422 mL	0.2110 mL	0.4221 mL	1.0552 mL
	100 mM	0.0338 mL	0.1688 mL	0.3377 mL	0.8442 mL

Meclofenamic acid Related Classifications

Neurological Disease Metabolic Disease Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Meclofenamic acid644-62-2MeclofenamateFat Mass and Obesity-associated Protein (FTO)Potassium ChannelGap Junction ProteinEndogenous MetaboliteKcsApotassium channelK+ channelvoltage-gatedhKv2.1anti-inflammatoryRGCsgap junctionInhibitorinhibitorinhibit

Productos vistos recientemente:

Nombre del producto

Requested Quantity *

Nombre del solicitante *

Saludo

Direcciòn del E-mail *

Número de teléfono *

Department

Nombre de la Organizaciòn *

City

Provincia

Country or Region *

Observaciones

Nombre del producto

Lot #

Nombre del solicitante *

Direcciòn del E-mail *

Número de teléfono

Department *

Nombre de la Organizaciòn *

Country or Region *

Observaciones

Name
E-mail *

	Sorry, but the email address you supplied was invalid.

Meclofenamic acid (Synonyms: Meclofenamate)

Revisión del cliente

Other Forms of Meclofenamic acid:

Meclofenamic acid Related Antibodies

8 Publications Citing Use of MCE Meclofenamic acid

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas Endogenous Metabolite:

Actividad biológica

Pureza y Documentación

Referencias

Revisión del cliente

Meclofenamic acid Related Antibodies

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

Meclofenamic acid Related Classifications

Keywords:

Productos vistos recientemente:

Revisión del cliente

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report