N-alpha-Tosyl-Lys chloromethyl ketone hydrochloride  (Synonyms: TLCK hydrochloride)

N-alpha-Tosyl-Lys chloromethyl ketone hydrochloride (TLCK hydrochloride) is a Serine protease inhibitor with an IC₅₀ of 12.0 μM against Caspase-3, 54.5 μM against Caspase-6, and 19.3 μM against Caspase-7. It inhibits thrombin-induced nuclear translocation of FOXO1. N-alpha-Tosyl-Lys chloromethyl ketone hydrochloride can be used in research related to leukemia and cardiovascular diseases^[1].

N-alpha-Tosyl-Lys chloromethyl ketone (15 min) hydrochloride is a potent inhibitor that acts on recombinant caspase-3 (IC₅₀ = 12.0 μM), caspase-6 (IC₅₀ = 54.5 μM), and caspase-7 (IC₅₀ = 19.3 μM)^[1].
At concentrations up to 120 μM, N-alpha-Tosyl-Lys chloromethyl ketone hydrochloride (20-120 μM; 24 h) does not significantly reduce the viability of mouse cardiac microvascular endothelial cells after 24 h of incubation, and a concentration of 100 μM is selected for subsequent studies^[2].
Hydrochloride salt of N-alpha-Tosyl-Lys chloromethyl ketone (100 μM; 2 h) slightly reduces thrombin-induced intracellular Ca²⁺ elevation in mouse cardiac microvascular endothelial cells, but does not significantly decrease the total intracellular Ca²⁺ concentration^[2].
N-alpha-Tosyl-Lys chloromethyl ketone hydrochloride inhibits thrombin-induced nuclear translocation of FOXO1, but does not inhibit the nuclear translocation of phosphorylated STAT3 (p-STAT3), in mouse cardiac microvascular endothelial cells^[2].
Hydrochloride of N-alpha-Tosyl-Lys chloromethyl ketone (100 μM) fails to reduce the nuclear fluorescence intensity of FOXO1 or phosphorylated STAT3 (p-STAT3) in thrombin-induced mouse cardiac microvascular endothelial cells at a concentration of 100 μM^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

369.31

C₁₄H₂₂Cl₂N₂O₃S

4238-41-9

O=S(C1=CC=C(C)C=C1)(NC(C(CCl)=O)CCCCN)=O.Cl

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Frydrych I, et al. Serine protease inhibitors N-alpha-tosyl-L-lysinyl-chloromethylketone (TLCK) and N-tosyl-L-phenylalaninyl-chloromethylketone (TPCK) are potent inhibitors of activated caspase proteases. J Cell Biochem. 2008;103(5):1646-1656.  [Content Brief]

  [2]. Lu YN, et al. Urinary trypsin inhibitor exerts multifaceted regulation of angiopoietin 2 in septic cardiomyopathy. Eur J Pharmacol. 2025;1003:177944.