2. PI3K/Akt/mTOR NF-κB Metabolic Enzyme/Protease Immunology/Inflammation Apoptosis
  3. Akt Reactive Oxygen Species Apoptosis
  4. KP372-1

KP372-1 is an Akt inhibitor that inhibits proliferation and induces apoptosis and anoikis. KP372-1 is also an NQO1 redox cycling agent that causes DNA damage (including DNA breakage) by generating ROS. KP372-1 can be used in cancer research (such as head and neck squamous cell carcinoma (HNSCC) and pancreatic cancer).

For research use only. We do not sell to patients.

KP372-1 Chemical Structure

KP372-1 Chemical Structure

CAS No. : 1374996-60-7

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Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 308 In-stock
Solution
10 mM * 1 mL in DMSO USD 308 In-stock
Solid
5 mg USD 280 In-stock
10 mg USD 480 In-stock
25 mg USD 960 In-stock
50 mg USD 1440 In-stock
100 mg USD 2160 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of KP372-1:

KP372-1 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE KP372-1

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  • Purity & Documentation

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Description

KP372-1 is an Akt inhibitor that inhibits proliferation and induces apoptosis and anoikis. KP372-1 is also an NQO1 redox cycling agent that causes DNA damage (including DNA breakage) by generating ROS. KP372-1 can be used in cancer research (such as head and neck squamous cell carcinoma (HNSCC) and pancreatic cancer)[1][2][3].

In Vitro

KP372-1 (0.0625, 0.125, 0.25, 0.5, 1.0 µM; 48 h) inhibits growth of JMARc42 and Tu167c2 cells with IC50s of 200 and 100 nM, respectively[1].
KP372-1 (125 nM; 24 h) induces Tu167c2 cells apoptosis and induces anoikis in the JMARc42 cells[1].
KP372-1 (125 nM; 30 min) blocks Akt, thereby decreasing the phosphorylation of the S6 ribosomal protein in both Tu167 and JMAR cells[1].
KP372-1 (0.250, 0.5, 1.0 µM; 30 min) inhibits Akt kinase activity with an IC50 of 250 nM in JMAR cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

KP372-1 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: JMARc42 and Tu167c2 cells
Concentration: 0.0625, 0.125, 0.25, 0.5, 1.0 µM
Incubation Time: 48 h
Result: Showed antiproliferative activity.

Apoptosis Analysis[1]

Cell Line: Tu167c2 and JMARc42 cells
Concentration: 125 nM
Incubation Time: 24 h
Result: Induced approximately 90% of cells apoptosis.

Western Blot Analysis[1]

Cell Line: Tu167 and JMAR cells
Concentration: 125 nM
Incubation Time: 30 min
Result: Induced a small but consistent decrease in Akt phosphorylation with a concomitant marked decrease in S6 phosphorylation.
Inhibited the EGF induced phosphorylation of Aktser473 in Tu167 and AktThr308 in JMAR.

Western Blot Analysis[1]

Cell Line: JMAR cells
Concentration: 0.250, 0.5, 1.0 µM
Incubation Time: 30 min
Result: Significantly blocked Akt kinase activity in a dose-dependent fashion, with an IC50 of 250 nM.
In Vivo

KP372-1(10, 20 mg/kg; i.v.; single daily for 33 days) induces NADH oxidation and impairs tumor growth in vivo without apparent toxicity[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Nude mice (H1299 xenografts model)[2].
Dosage: 10, 20 mg/kg
Administration: Tailvein injection; single daily for 33 days
Result: Affected tumor metabolism and suppressed tumor growth.
Molecular Weight

448.36

Formula

C20H8N12O2
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C2=C(C3=NC4=NN=NN4N=C31)C=CC=C2.O=C5C6=NC7=NN=NN7N=C6C8=C5C=CC=C8
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 17.86 mg/mL (39.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2304 mL 11.1518 mL 22.3035 mL
5 mM 0.4461 mL 2.2304 mL 4.4607 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.75%

COA (262 KB) LCMS (100 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2304 mL 11.1518 mL 22.3035 mL 55.7588 mL
5 mM 0.4461 mL 2.2304 mL 4.4607 mL 11.1518 mL
10 mM 0.2230 mL 1.1152 mL 2.2304 mL 5.5759 mL
15 mM 0.1487 mL 0.7435 mL 1.4869 mL 3.7173 mL
20 mM 0.1115 mL 0.5576 mL 1.1152 mL 2.7879 mL
25 mM 0.0892 mL 0.4461 mL 0.8921 mL 2.2304 mL
30 mM 0.0743 mL 0.3717 mL 0.7435 mL 1.8586 mL
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KP372-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

KP372-11374996-60-7AktReactive Oxygen SpeciesApoptosisPKBProtein kinase Bpancreatic cancerHNSCCJMARc42Tu167c2Tu167cancerROSapoptosisInhibitorinhibitorinhibit

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