1. Cell Cycle/DNA Damage
    Epigenetics
  2. HDAC
  3. Splitomicin

Splitomicin (Synonyms: Splitomycin)

Cat. No.: HY-100585 Purity: 98.42%
Handling Instructions

Splitomicin (Splitomycin) is a selective Sir2p inhibitor. Splitomicin inhibits NAD+-dependent HDAC activity of Sir2 protein. Splitomicin induces dose-dependent inhibition of HDAC in the yeast extract with an IC50 of 60 μM.

For research use only. We do not sell to patients.

Splitomicin Chemical Structure

Splitomicin Chemical Structure

CAS No. : 5690-03-9

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
5 mg USD 50 In-stock
Estimated Time of Arrival: December 31
10 mg USD 70 In-stock
Estimated Time of Arrival: December 31
25 mg USD 140 In-stock
Estimated Time of Arrival: December 31
50 mg USD 220 In-stock
Estimated Time of Arrival: December 31
100 mg USD 420 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Splitomicin (Splitomycin) is a selective Sir2p inhibitor. Splitomicin inhibits NAD+-dependent HDAC activity of Sir2 protein. Splitomicin induces dose-dependent inhibition of HDAC in the yeast extract with an IC50 of 60 μM[1].

IC50 & Target[1]

Sir2p

60 μM (IC50)

In Vitro

Splitomicin (10-333 μM; 24 hours) elicits antiproliferative effects in MCF-7 and H1299 cells in a dose-dependent manner in colony formation assay. Splitomicin (33 μM) fails to decrease the number of colonies, but Splitomicin (100 and 333 μM) effectively inhibits colony formation in MCF-7 and H1299 cells[2].

Cell Proliferation Assay[2]

Cell Line: Human breast cancer MCF-7 and lung cancer H1299 cells
Concentration: 10, 33, 100, and 333 μM
Incubation Time: 24 hours
Result: Inhibited colony formation in a dose-dependent manner.
In Vivo

Splitomicin (80 mg/kg with an intraperitoneal injection every 24 h for 5 days, in mice) enhances tissue factor (TF) activity in the arterial vessel wall and accelerates carotid artery thrombus formation in a photochemical injury model[3].

Animal Model: C57BL/6 mice aged 12-14 weeks weighing on average 27 g[3]
Dosage: 80 mg/kg
Administration: Intraperitoneal injection every 24 h for 5 days
Result: Increased TF activity in mouse carotid artery as compared with the controls.
Molecular Weight

198.22

Formula

C₁₃H₁₀O₂

CAS No.

5690-03-9

SMILES

O=C1CCC2=C3C=CC=CC3=CC=C2O1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (1261.22 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.0449 mL 25.2245 mL 50.4490 mL
5 mM 1.0090 mL 5.0449 mL 10.0898 mL
10 mM 0.5045 mL 2.5224 mL 5.0449 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (10.49 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (10.49 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (10.49 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product name

 

Salutation

Applicant name *

 

Email address *

Phone number *

 

Organization name *

Country or Region *

 

Requested quantity *

Remarks

Bulk Inquiry

Inquiry Information

Product name:
Splitomicin
Cat. No.:
HY-100585
Quantity:
MCE Japan Authorized Agent: