21-Hydroxypregnenolone

Name: 21-Hydroxypregnenolone
Brand: MedChemExpress
SKU: HY-113020
Rating: 4.5 (1 reviews)

Cat. No.: HY-113020 Purity: 98.05%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

21-Hydroxypregnenolone is a CYP17 inhibitor. 21-Hydroxypregnenolone competitively inhibits CYP17-mediated 17α-hydroxylation activity of progesterone, with a Ki value of 36.4 μM.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

21-Hydroxypregnenolone 화학구조

CAS No. : 1164-98-3

사이즈	재고	수량

무료 샘플 (0.1 - 0.2 mg)	지금 신청하기
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	견적 받기
100 mg	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 publication(s) in Google Scholar

Other Forms of 21-Hydroxypregnenolone:

21-Hydroxypregnenolone (Standard) 견적 받기

1 Publications Citing Use of MCE 21-Hydroxypregnenolone

•Cell Metab. 2024 Nov 5;36(11):2402-2418.e10. [Abstract]

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

View All Isoforms

Human Endogenous Metabolite Microbial Metabolite

View All Cytochrome P450 Isoform Specific Products:

View All Isoforms

CYP1 CYP2 CYP3 CYP4 CYP11 CYP17 Aromatase/CYP19A1 CYP26 CYP51 CYP1A2 CYP2D6 CYP2C9 CYP2C19 CYP3A CYP3A4 CYP17A1

Biological Activity
순도&문서
References
고객리뷰

제품 설명

21-Hydroxypregnenolone is a CYP17 inhibitor. 21-Hydroxypregnenolone competitively inhibits CYP17-mediated 17α-hydroxylation activity of progesterone, with a Ki value of 36.4 μM^[1].

IC₅₀ & Target^[1]

Human Endogenous Metabolite

CYP17

In Vitro

21-Hydroxypregnenolone (20-100 μM; 10 min) competitively inhibits progesterone 17α-hydroxylation by recombinant human CYP17 expressed in E. coli membrane fractions with a K_i value of 36.4 μM, causing 81% inhibition at 100 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

332.48

화학식

C₂₁H₃₂O₃

CAS No.

1164-98-3

Appearance

Solid

Color

White to off-white

SMILES

C[C@]12[C@]([H])([C@]3([C@]([H])(CC2)[C@]4(C)C(C[C@H](CC4)O)=CC3)[H])CC[C@@H]1C(CO)=O

Structure Classification

Steroids

Initial Source

Endogenous metabolite

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

용액&용해도

In Vitro:

DMSO : ≥ 25 mg/mL (75.19 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0077 mL	15.0385 mL	30.0770 mL
5 mM	0.6015 mL	3.0077 mL	6.0154 mL
10 mM	0.3008 mL	1.5038 mL	3.0077 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2 mg/mL (6.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2 mg/mL (6.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 98.05%

Select Batch:

Data Sheet (274 KB) SDS (251 KB)

COA (250 KB) LCMS (91 KB)

Handling Instructions (2659 KB)

References

[1]. Niwa T, et al. Inhibition of human steroidogenic cytochrome P450 c17 by 21-hydroxypregnenolone and related steroid hormones. Biol Pharm Bull. 2012;35(9):1594-1597.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.0077 mL	15.0385 mL	30.0770 mL	75.1925 mL
	5 mM	0.6015 mL	3.0077 mL	6.0154 mL	15.0385 mL
	10 mM	0.3008 mL	1.5038 mL	3.0077 mL	7.5192 mL
	15 mM	0.2005 mL	1.0026 mL	2.0051 mL	5.0128 mL
	20 mM	0.1504 mL	0.7519 mL	1.5038 mL	3.7596 mL
	25 mM	0.1203 mL	0.6015 mL	1.2031 mL	3.0077 mL
	30 mM	0.1003 mL	0.5013 mL	1.0026 mL	2.5064 mL
	40 mM	0.0752 mL	0.3760 mL	0.7519 mL	1.8798 mL
	50 mM	0.0602 mL	0.3008 mL	0.6015 mL	1.5038 mL
	60 mM	0.0501 mL	0.2506 mL	0.5013 mL	1.2532 mL