4-Aminosalicylic acid

Name: 4-Aminosalicylic acid
Brand: MedChemExpress
SKU: HY-I0447
Rating: 4.5 (1 reviews)

製品番号: HY-I0447 純度: 99.82%: Data Sheet SDS COA 取扱説明書
FAQs
Technical Support

4-Aminosalicylic acid is an inhibitor for dihydrofolate reductase (DHFR) and an antituberculous agent. 4-Aminosalicylic acid exhibits anti-inflammatory activity and can be used in research of inflammatory bowel disease (IBD). 4-Aminosalicylic acid is orally active.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

4-Aminosalicylic acid 構造式

CAS 番号 : 65-49-6

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 27	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 27	在庫あり	Estimated Time of Arrival: December 31
Solid
5 g	$25	在庫あり	Estimated Time of Arrival: December 31
10 g		お問い合わせ
50 g		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 1 publication(s) in Google Scholar

Other Forms of 4-Aminosalicylic acid:

Sodium 4-aminosalicylate dihydrate 在庫あり
4-Aminosalicylic acid-d₆ お問い合わせ
4-Aminosalicylic acid hemicalcium お問い合わせ
4-Aminosalicylic acid (Standard) お問い合わせ

顧客検証

•Nature. 2025 Jul;643(8070):192-200.

Antibiotic アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	IC₅₀	2240 3 Compound: PAS	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 24 hrs by MTS assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 24 hrs by MTS assay	[PMID: 24953953]
HepG2	IC₅₀	2240 3 Compound: PAS	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 25593095]
HepG2	IC₅₀	2240 3 Compound: PAS	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 24 hrs by MTS assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 24 hrs by MTS assay	[PMID: 24953953]
HepG2	IC₅₀	2240 3 Compound: PAS	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 24 hrs by MTS assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 24 hrs by MTS assay	[PMID: 24953953]
HepG2	IC₅₀	2240 3 Compound: PAS	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 25593095]
HepG2	IC₅₀	2240 3 Compound: PAS	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 25593095]
HepG2	IC₅₀	2240 3 Compound: PAS	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after overnight incubation by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after overnight incubation by MTT assay	[PMID: 31401536]
HepG2	IC₅₀	2240 3 Compound: PAS	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after overnight incubation by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after overnight incubation by MTT assay	[PMID: 31401536]
HepG2	IC₅₀	2240 3 Compound: PAS	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after overnight incubation by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after overnight incubation by MTT assay	[PMID: 31401536]
HepG2	IC₅₀	> 1270 3 Compound: PAS	Cytotoxicity against human HepG2 cells assessed as inhibition of cell viability Cytotoxicity against human HepG2 cells assessed as inhibition of cell viability	[PMID: 37423128]

体外実験

4-Aminosalicylic acid is incorporated into the folate pathway via dihydropteroate synthase (DHPS) and dihydrofolate synthase (DHFS) to generate the hydroxydihydrofolate antimetabolite, which in turn inhibits DHFR enzyme activity^[1].
4-Aminosalicylic acid inhibits M. tuberculosis H37Rv growth with MIC value of 0.4 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

153.14

分子式

C₇H₇NO₃

CAS 番号

65-49-6

Appearance

Solid

Color

Off-white to light brown

SMILES

O=C(O)C1=CC=C(N)C=C1O

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Store at room temperature 3 years

*In solvent : -80°C, 6 months; -20°C, 1 month (Store at room temperature 3 years)

溶剤 & 溶解度

体外:

DMSO : 100 mg/mL (653.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.5300 mL	32.6499 mL	65.2997 mL
5 mM	1.3060 mL	6.5300 mL	13.0599 mL
10 mM	0.6530 mL	3.2650 mL	6.5300 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (16.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (16.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (16.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.82%

Select Batch:

データシート (273 KB) SDS (393 KB)

COA (247 KB) HNMR (132 KB) RP-HPLC (240 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Zheng J, et al. para-Aminosalicylic acid is a prodrug targeting dihydrofolate reductase in Mycobacterium tuberculosis. J Biol Chem. 2013 Aug 9;288(32):23447-56. [Content Brief]

[2]. Abdu-Allah HHM, et al. Conjugation of 4-aminosalicylate with thiazolinones afforded non-cytotoxic potent in vitro and in vivo anti-inflammatory hybrids. Bioorg Chem. 2020 Jan;94:103378. [Content Brief]

[3]. Dhaneshwar SS. Colon-specific prodrugs of 4-aminosalicylic acid for inflammatory bowel disease. World J Gastroenterol. 2014 Apr 7;20(13):3564-71. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.5300 mL	32.6499 mL	65.2997 mL	163.2493 mL
	5 mM	1.3060 mL	6.5300 mL	13.0599 mL	32.6499 mL
	10 mM	0.6530 mL	3.2650 mL	6.5300 mL	16.3249 mL
	15 mM	0.4353 mL	2.1767 mL	4.3533 mL	10.8833 mL
	20 mM	0.3265 mL	1.6325 mL	3.2650 mL	8.1625 mL
	25 mM	0.2612 mL	1.3060 mL	2.6120 mL	6.5300 mL
	30 mM	0.2177 mL	1.0883 mL	2.1767 mL	5.4416 mL
	40 mM	0.1632 mL	0.8162 mL	1.6325 mL	4.0812 mL
	50 mM	0.1306 mL	0.6530 mL	1.3060 mL	3.2650 mL
	60 mM	0.1088 mL	0.5442 mL	1.0883 mL	2.7208 mL
	80 mM	0.0816 mL	0.4081 mL	0.8162 mL	2.0406 mL
	100 mM	0.0653 mL	0.3265 mL	0.6530 mL	1.6325 mL