4'-Methoxychalcone

Name: 4'-Methoxychalcone
Brand: MedChemExpress
SKU: HY-128400
Rating: 4.5 (0 reviews)

製品番号: HY-128400 純度: 99.82%: Data Sheet SDS COA 取扱説明書
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4'-Methoxychalcone regulates adipocyte differentiation through PPARγ activation. 4'-Methoxychalcone modulates the expression and secretion of various adipokines in adipose tissue that are involved in insulin sensitivity.

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4'-Methoxychalcone 構造式

CAS 番号 : 959-23-9

容量	価格（税別）	在庫状況	数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 27	在庫あり	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 27	在庫あり	Estimated Time of Arrival: December 31
Solid
5 g	$25	在庫あり	Estimated Time of Arrival: December 31
10 g	$42	在庫あり	Estimated Time of Arrival: December 31
25 g	$88	在庫あり	Estimated Time of Arrival: December 31
50 g	$147	在庫あり	Estimated Time of Arrival: December 31
100 g	$245	在庫あり	Estimated Time of Arrival: December 31
> 100 g		お問い合わせ

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カスタマーレビュー

Based on 0 publication(s) in Google Scholar

Other Forms of 4'-Methoxychalcone:

4'-Methoxychalcone (Standard) お問い合わせ

PARP アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示

PARP PARP1 PARP2 PARP3 PARP4 TNKS1/PARP5A TNKS2/PARP5B PARP7 PARP10 PARP14 PARP15 PARP12 PARP16

生物活性
純度とドキュメンテーション
参考文献
カスタマーレビュー

製品説明

IC₅₀ & Target

PPARγ^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	GI₅₀	6.8 μM Compound: 17	Antiproliferative activity against human A2780 cells assessed as cell growth inhibition measured after 72 hrs by trypan blue assay Antiproliferative activity against human A2780 cells assessed as cell growth inhibition measured after 72 hrs by trypan blue assay	[PMID: 34262643]
CAL-51	GI₅₀	3.33 μM Compound: SSE14109	Antiproliferative activity against human CAL51 cells assessed as growth inhibition after 3 days by SRB assay Antiproliferative activity against human CAL51 cells assessed as growth inhibition after 3 days by SRB assay	[PMID: 28743509]
DLD-1	EC₅₀	35 μM Compound: 7	Cytotoxicity against human DLD-1 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay Cytotoxicity against human DLD-1 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay	[PMID: 36356534]
FHC	CC₅₀	62 μM Compound: 7	Cytotoxicity against human FHC cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay Cytotoxicity against human FHC cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay	[PMID: 36356534]
HCT-116	EC₅₀	16 μM Compound: 7	Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay	[PMID: 36356534]
HCT-116	EC₅₀	17 μM Compound: 7	Cytotoxicity against p53-/- human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay Cytotoxicity against p53-/- human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay	[PMID: 36356534]
HCT-116	GI₅₀	3.57 μM Compound: SSE14109	Antiproliferative activity against human HCT116 cells assessed as growth inhibition after 3 days by SRB assay Antiproliferative activity against human HCT116 cells assessed as growth inhibition after 3 days by SRB assay	[PMID: 28743509]
HT-29	EC₅₀	23 μM Compound: 7	Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay	[PMID: 36356534]
HT-29	GI₅₀	11.7 μM Compound: 17	Antiproliferative activity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by trypan blue assay Antiproliferative activity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by trypan blue assay	[PMID: 34262643]
L929	CC₅₀	50.4 μM Compound: 13	Cytotoxicity in mouse NCTC-929 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity in mouse NCTC-929 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31000155]
MSTO-211H	GI₅₀	11.7 μM Compound: 17	Antiproliferative activity against human MSTO-211H cells assessed as cell growth inhibition measured after 72 hrs by trypan blue assay Antiproliferative activity against human MSTO-211H cells assessed as cell growth inhibition measured after 72 hrs by trypan blue assay	[PMID: 34262643]

体外実験

4'-Methoxychalcone (5 μM; 8 days) markedly increases the mRNA expression of adipogenic genes during adipocyte differentiation, PPARγ, aP2, FAS, adiponectin and GluT 4^[1].
4'-Methoxychalcone (5 μM; 8 days) reduces the upregulated mRNA expression of IL-6, PAI-1, and MCP-1 by TNF-α in preadipocyte 3T3-L1 cells^[1].
4'-Methoxychalcone (5 μM; 0-8 days) causes an increase in PPARγ expression during differentiation, while C/EBPβ protein expression is relatively unaffected^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:	Preadipocyte 3T3-L1 cells
Concentration:	5 μM
Incubation Time:	0 day; 2 days; 4 days; 6 days; 8 days
Result:	Increased the mRNA expression of all adipogenic genes, except GluT1 in adipocyte.

Western Blot Analysis^[1]

Cell Line:	Preadipocyte 3T3-L1 cells
Concentration:	5 μM
Incubation Time:	8 days
Result:	Increased PPARγ expression in adipocyte.

分子量

238.28

分子式

C₁₆H₁₄O₂

CAS 番号

959-23-9

Appearance

Solid

Color

White to light yellow

SMILES

O=C(C1=CC=C(OC)C=C1)/C=C/C2=CC=CC=C2

Structure Classification

Initial Source

輸送条件

Room temperature in continental US; may vary elsewhere.

保管条件

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶剤 & 溶解度

体外:

DMSO : 50 mg/mL (209.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.1967 mL	20.9837 mL	41.9674 mL
5 mM	0.8393 mL	4.1967 mL	8.3935 mL
10 mM	0.4197 mL	2.0984 mL	4.1967 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

一般には略語で表示されます：C₁V₁ = C₂V₂

濃度 _（開始）

体積 _（開始）

濃度 _（終了）

体積 _（終了）

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (10.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

純度とドキュメンテーション

純度: 99.82%

Select Batch:

データシート (272 KB) SDS (393 KB)

COA (245 KB) HNMR (228 KB) LCMS (88 KB)

取扱説明書 (2659 KB)

参考文献

[1]. Han Y, et al. Regulatory effects of 4-methoxychalcone on adipocyte differentiation through PPARγ activation and reverse effect on TNF-α in 3T3-L1 cells. Food Chem Toxicol. 2017 Aug;106(Pt A):17-24. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.1967 mL	20.9837 mL	41.9674 mL	104.9186 mL
	5 mM	0.8393 mL	4.1967 mL	8.3935 mL	20.9837 mL
	10 mM	0.4197 mL	2.0984 mL	4.1967 mL	10.4919 mL
	15 mM	0.2798 mL	1.3989 mL	2.7978 mL	6.9946 mL
	20 mM	0.2098 mL	1.0492 mL	2.0984 mL	5.2459 mL
	25 mM	0.1679 mL	0.8393 mL	1.6787 mL	4.1967 mL
	30 mM	0.1399 mL	0.6995 mL	1.3989 mL	3.4973 mL
	40 mM	0.1049 mL	0.5246 mL	1.0492 mL	2.6230 mL
	50 mM	0.0839 mL	0.4197 mL	0.8393 mL	2.0984 mL
	60 mM	0.0699 mL	0.3497 mL	0.6995 mL	1.7486 mL
	80 mM	0.0525 mL	0.2623 mL	0.5246 mL	1.3115 mL
	100 mM	0.0420 mL	0.2098 mL	0.4197 mL	1.0492 mL