2. Cell Cycle/DNA Damage Epigenetics
  3. HDAC
  4. M344

M344  (Synonyms: D 237; MS 344; N-(7-(hydroxyamino)-7-oxoheptyl)benzamide)

製品番号: HY-13506 純度: 99.30%
COA 取扱説明書 Technical Support

M344 (D 237) is an inhibitor of histone deacetylase (IC50=100 nM) and an inducer of terminal cell fifferentiation.

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M344

M344 構造式

CAS 番号 : 251456-60-7

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 66 在庫あり
Solution
10 mM * 1 mL in DMSO USD 66 在庫あり
Solid
5 mg $60 在庫あり
10 mg $102 在庫あり
25 mg $235 在庫あり
50 mg $432 在庫あり
100 mg $645 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 2 publication(s) in Google Scholar

Other Forms of M344:

M344 関連抗体

Top Publications Citing Use of Products

HDAC アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

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製品説明

M344 (D 237) is an inhibitor of histone deacetylase (IC50=100 nM) and an inducer of terminal cell fifferentiation.

IC50 & Target[1]

HDAC

100 nM (IC50)

Cellular Effect
Cell Line Type Value Description References
A-431 IC50
2.3 μM
Compound: 23
Concentration required to inhibit the epidermoid A431 cell growth by 50%, P53 status of cell line used was wild-type.
Concentration required to inhibit the epidermoid A431 cell growth by 50%, P53 status of cell line used was wild-type.
[PMID: 11831887]
A549 IC50
0.8 μM
Compound: 23
Concentration required to inhibit the lung A549 cell growth by 50%,P53 status of cell line used was wild-type.
Concentration required to inhibit the lung A549 cell growth by 50%,P53 status of cell line used was wild-type.
[PMID: 11831887]
HCT-116 IC50
0.2 μM
Compound: 23
Concentration required to inhibit the colon HCT116 cell growth by 50%,P53 status of cell line used was wild-type.
Concentration required to inhibit the colon HCT116 cell growth by 50%,P53 status of cell line used was wild-type.
[PMID: 11831887]
HCT-116 IC50
0.21 μM
Compound: 23
Concentration required to inhibit the HCT116 cell growth by 50%.
Concentration required to inhibit the HCT116 cell growth by 50%.
[PMID: 11831887]
LS174T IC50
1.5 μM
Compound: 23
Concentration required to inhibit the colon LS 174T cell growth by 50%,P53 status of cell line used was mutant-type.
Concentration required to inhibit the colon LS 174T cell growth by 50%,P53 status of cell line used was mutant-type.
[PMID: 11831887]
NCI-H1299 IC50
3.9 μM
Compound: 23
Concentration required to inhibit the lung H1299 cell growth by 50%,P53 status of cell line used was wild-type.
Concentration required to inhibit the lung H1299 cell growth by 50%,P53 status of cell line used was wild-type.
[PMID: 11831887]
SW-620 IC50
0.7 μM
Compound: 23
Concentration required to inhibit the colon SW 620 cell growth by 50%,P53 status of cell line used was mutant-type.
Concentration required to inhibit the colon SW 620 cell growth by 50%,P53 status of cell line used was mutant-type.
[PMID: 11831887]
T-24 IC50
3.8 μM
Compound: 23
Concentration required to inhibit the bladder T24 cell growth by 50%,P53 status of cell line used was wild-type.
Concentration required to inhibit the bladder T24 cell growth by 50%,P53 status of cell line used was wild-type.
[PMID: 11831887]
体外実験

M344 is a potential histone deacetylase (HDAC) inhibitor. With increasing concentrations of M344, there is a dose dependant decrease in BRCA1 mRNA and treatment with both 1 and 5 μM concentrations of M344 resulting in a significant decrease in BRCA1 expression in all cell lines examined. M344 in combination with Cisplatin leads to a decrease in BRCA1 mRNA expression as compared to Cisplatin treatment alone in all cell lines with the exception of A2780s, which is recognized as having potent cytotoxicity to Cisplatin. In the MCF7 cell line, BRCA1 is down regulated at physiological doses of M344 (0.5 μM and 1 μM) but M344 does not have the same inhibitory effect on BRCA1 at the 5.0 μM dose. Co-treatment with Cisplatin and increasing concentrations of M344 reduces BRCA1 protein levels in all breast and ovarian cell lines examined[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

M344 関連抗体
分子量

307.39

分子式

C16H25N3O3
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

O=C(NCCCCCCC(NO)=O)C1=CC=C(N(C)C)C=C1
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : ≥ 100 mg/mL (325.32 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2532 mL 16.2660 mL 32.5320 mL
5 mM 0.6506 mL 3.2532 mL 6.5064 mL
10 mM 0.3253 mL 1.6266 mL 3.2532 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.13 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.13 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  50% PEG300    50% Saline

    Solubility: 10 mg/mL (32.53 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.30%

COA (241 KB) HNMR (254 KB) RP-HPLC (258 KB) MS (70 KB)

取扱説明書 (2659 KB)
参考文献
細胞実験
[2]

The A2780s and A2780cp cell lines, the T-47D and OVCAR-4 cell lines. and the MCF7 and HCC1937cell lines, are maintained in Dulbecco's-MEM supplemented with 10% fetal bovine serum and 100 μg/mL penicillin-streptomycin. Unless otherwise described, cells are treated for 24 hrs with 2 μg/mL Cisplatin alone, and in combination with the HDAC inhibitor M344 at concentrations of 0.5, 1.0, or 5.0 μM. Phase contrast images are collected using the 10× objective of an Eclipse TE2000-U. Cell viability is measured by the MTT rapid colorimetric assay. Approximately 4,500 cells are seeded into each well of a 96-well flat bottom plate. The cells are incubated overnight to allow for cell attachment. Cells are then treated with Cisplatin in concentrations of 0-8 μg/mL alone or in combination with 1 μM of the HDAC inhibitor, M344. Forty eight hours following treatment, 42 μL of a 5 mg/mL MTT substrate solution in phosphate buffered saline (PBS) is added and incubated for up to 4 hrs at 37°C. The resulting violet formazan precipitate is solubilized by the addition of 82 μL of a 0.01 M HCl/10% SDS solution and plates are incubated overnight at 37°C. The plates are then analyzed on an MRX Microplate Reader at 570 nm to determine the optical density of the samples[2].

MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2532 mL 16.2660 mL 32.5320 mL 81.3299 mL
5 mM 0.6506 mL 3.2532 mL 6.5064 mL 16.2660 mL
10 mM 0.3253 mL 1.6266 mL 3.2532 mL 8.1330 mL
15 mM 0.2169 mL 1.0844 mL 2.1688 mL 5.4220 mL
20 mM 0.1627 mL 0.8133 mL 1.6266 mL 4.0665 mL
25 mM 0.1301 mL 0.6506 mL 1.3013 mL 3.2532 mL
30 mM 0.1084 mL 0.5422 mL 1.0844 mL 2.7110 mL
40 mM 0.0813 mL 0.4066 mL 0.8133 mL 2.0332 mL
50 mM 0.0651 mL 0.3253 mL 0.6506 mL 1.6266 mL
60 mM 0.0542 mL 0.2711 mL 0.5422 mL 1.3555 mL
80 mM 0.0407 mL 0.2033 mL 0.4066 mL 1.0166 mL
100 mM 0.0325 mL 0.1627 mL 0.3253 mL 0.8133 mL
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M344 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

M344251456-60-7D 237 MS 344M 344M-344D237D-237MS344MS 344MS-344HDACHistone deacetylasesInhibitorinhibitorinhibit

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