2. Metabolic Enzyme/Protease Anti-infection
  3. Adenosine Deaminase Antibiotic
  4. Pentostatin

ペントスタチン  (Synonyms: Pentostatin; Deoxycoformycin; CI-825)

製品番号: HY-A0006 純度: 99.82%
COA 取扱説明書 Technical Support

Pentostatin (CI-825; Deoxycoformycin) is an irreversible inhibitor of adenosine deaminase with Ki of 2.5 pM.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 53910-25-1

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 82 在庫あり
Solution
10 mM * 1 mL in DMSO USD 82 在庫あり
Solid
5 mg $75 在庫あり
10 mg $120 在庫あり
25 mg $285 在庫あり
50 mg $542 在庫あり
100 mg $870 在庫あり
500 mg $2610 在庫あり
1 g   お問い合わせ  
5 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 10 publication(s) in Google Scholar

Other Forms of Pentostatin:

ペントスタチン 関連抗体

Top Publications Citing Use of Products

    Pentostatin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jun 14:e2306514.  [Abstract]

    Pentostatin (Deoxycoformycin; DCF) (0.2 mg/kg; i.c.) elevated ICPs of C57BL/6 mice.

    Pentostatin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jun 14:e2306514.  [Abstract]

    Pentostatin (Deoxycoformycin; DCF) (0.2 mg/kg; i.c.) elevated AUCs of C57BL/6 mice.

    Pentostatin purchased from MedChemExpress. Usage Cited in: Virulence. 2018;9(1):1509-1520.  [Abstract]

    The viability of RAW 264.7 cells treated with or without Pentostatin (Deoxycoformycin; dCF) (100 μM).

    Pentostatin purchased from MedChemExpress. Usage Cited in: Virulence. 2018;9(1):1509-1520.  [Abstract]

    Pentostatin (Deoxycoformycin; dCF) (100 μM; 12-24 h) induced a detectable band of active caspase-3 in mouse macrophages RAW 264.7 treated with dAdo.

    Pentostatin purchased from MedChemExpress. Usage Cited in: Virulence. 2018;9(1):1509-1520.  [Abstract]

    Pentostatin (Deoxycoformycin; dCF) (100 μM; 12 h) induced a detectable band of active caspase-3 in mouse macrophages RAW 264.7 treated with tNT+dAMP.

    Antibiotic アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

    • 生物活性

    • プロトコル

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Pentostatin (CI-825; Deoxycoformycin) is an irreversible inhibitor of adenosine deaminase with Ki of 2.5 pM.

    IC50 & Target

    Ki: 2.5 pM (adenosine deaminase)
    Cellular Effect
    Cell Line Type Value Description References
    L1210 IC50
    1.9 3
    Compound: pentostatin
    Compound was evaluated for the cytotoxicity against L1210 cells in absence of coformycin
    Compound was evaluated for the cytotoxicity against L1210 cells in absence of coformycin
    		[PMID: 3489838]
    L1210 IC50
    0.25 3
    Compound: pentostatin
    Compound was evaluated for the cytotoxicity against L1210 cells in presence of coformycin
    Compound was evaluated for the cytotoxicity against L1210 cells in presence of coformycin
    		[PMID: 3489838]
    L1210 IC50
    0.25 3
    Compound: pentostatin
    Compound was evaluated for the cytotoxicity against L1210 cells in presence of coformycin
    Compound was evaluated for the cytotoxicity against L1210 cells in presence of coformycin
    		[PMID: 3489838]
    L1210 IC50
    1.9 3
    Compound: pentostatin
    Compound was evaluated for the cytotoxicity against L1210 cells in absence of coformycin
    Compound was evaluated for the cytotoxicity against L1210 cells in absence of coformycin
    		[PMID: 3489838]
    体内実験

    In ECP and Pentostatin (CI-825; Deoxycoformycin; 4 mg/m2, i.v.) treatment, all dogs develop granulocytopenia with <500 granulocytes/μL from day 4. Thrombocytopenia (<20,000 platelets/μL) occurs from day 7 after HCT with nadirs of 3000 to 14000 platelets/μL[1]. Pentostatin (2 mg/kg) in combination with cordycepin (2 mg/kg) is 100% effective in the T. evansi-infected mice. There is an increase in levels of some biochemical parameters, especially on liver enzymes, which are accompanied by histological lesions in the liver and kidneys. Pentostatin individually has no curative effect on infected groups[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    臨床実験
    分子量

    268.27

    分子式

    C11H16N4O4
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O[C@H]1C(N=CN2[C@H](O[C@@H]3CO)C[C@@H]3O)=C2NC=NC1
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    H2O : 100 mg/mL (372.75 mM; Need ultrasonic)

    DMSO : ≥ 50 mg/mL (186.38 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.7275 mL 18.6377 mL 37.2755 mL
    5 mM 0.7455 mL 3.7275 mL 7.4551 mL
    10 mM 0.3728 mL 1.8638 mL 3.7275 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (7.75 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (7.75 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  Saline

      Solubility: 100 mg/mL (372.75 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.93%

    COA (255 KB) HNMR (214 KB) LCMS (124 KB)

    取扱説明書 (2659 KB)
    参考文献
    動物実験
    [1]

    All recipient dogs are conditioned for transplantation by 920 cGy TBI at 7 cGy/minute using a linear accelerator. Dogs in group A1receive ECP administered on days −2 and −1 with TBI on day 0 and dogs in group A2receive ECP on days −6 and −5, intravenous (IV) infusion of pentostatin at a dose of 4 mg/m2 on days −4 and −3, and TBI on day 0. Donor marrow cells from DLA-nonidentical donors are aspirated under general anesthesia through needles inserted into humeri and femora and stored in heparinized tissue culture medium at 4°C for no more than 6 hours. Within 4 hours of TBI, harvested marrow cells are infused IV into recipients at a median dose of 2.9 (range, 1.9 to 6.1) ×108 total nucleated cells (TNC)/kg. The day of marrow grafting is designated as day 0. In addition to marrow graft, recipients are given IV infusions of peripheral blood buffy coat cells obtained by leukapheresis from the marrow donor on days 1 and 2, at a median dose of 2.3 (range, 1.2 to 6.9) ×108 TNC/kg to ensure consistent hematopoietic engraftment. MTX, at a dose of 0.4 mg/kg intravenously is used as postgrafting immunosuppression and administered on days +1, +3, +6 and +11, then weekly thereafter until day 102.

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 3.7275 mL 18.6377 mL 37.2755 mL 93.1887 mL
    5 mM 0.7455 mL 3.7275 mL 7.4551 mL 18.6377 mL
    10 mM 0.3728 mL 1.8638 mL 3.7275 mL 9.3189 mL
    15 mM 0.2485 mL 1.2425 mL 2.4850 mL 6.2126 mL
    20 mM 0.1864 mL 0.9319 mL 1.8638 mL 4.6594 mL
    25 mM 0.1491 mL 0.7455 mL 1.4910 mL 3.7275 mL
    30 mM 0.1243 mL 0.6213 mL 1.2425 mL 3.1063 mL
    40 mM 0.0932 mL 0.4659 mL 0.9319 mL 2.3297 mL
    50 mM 0.0746 mL 0.3728 mL 0.7455 mL 1.8638 mL
    60 mM 0.0621 mL 0.3106 mL 0.6213 mL 1.5531 mL
    80 mM 0.0466 mL 0.2330 mL 0.4659 mL 1.1649 mL
    100 mM 0.0373 mL 0.1864 mL 0.3728 mL 0.9319 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Pentostatin Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Pentostatin53910-25-1CI-825 DeoxycoformycinCI825CI 825Adenosine DeaminaseAntibioticInhibitorinhibitorinhibit

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