Pentostatin (Synonyms: CI-825; Deoxycoformycin)

Name: Pentostatin
Brand: MedChemExpress
SKU: HY-A0006
Rating: 5.0 (10 reviews)

Cat. No.: HY-A0006 Pureza: 99.82%: Ficha de datos
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Pentostatin (CI-825; Deoxycoformycin) is an irreversible inhibitor of adenosine deaminase with K_i of 2.5 pM.

Para uso exclusivo en investigación. No vendemos a pacientes.

No. CAS : 53910-25-1

Tamaño	Stock	Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
500 mg	En stock	Estimated Time of Arrival: December 31
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5 g	Obtener un presupuesto

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Revisión del cliente

Based on 10 publication(s) in Google Scholar

Other Forms of Pentostatin:

Pentostatin (Standard) Obtener un presupuesto

10 Publications Citing Use of MCE Pentostatin

In Vivo Efficacy Study

Cell Proliferation/Viability Assay

: Pentostatin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jun 14:e2306514. [Abstract]; Pentostatin (Deoxycoformycin; DCF) (0.2 mg/kg; i.c.) elevated ICPs of C57BL/6 mice.

: Pentostatin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Jun 14:e2306514. [Abstract]; Pentostatin (Deoxycoformycin; DCF) (0.2 mg/kg; i.c.) elevated AUCs of C57BL/6 mice.

: Pentostatin purchased from MedChemExpress. Usage Cited in: Virulence. 2018;9(1):1509-1520. [Abstract]; The viability of RAW 264.7 cells treated with or without Pentostatin (Deoxycoformycin; dCF) (100 μM).

: Pentostatin purchased from MedChemExpress. Usage Cited in: Virulence. 2018;9(1):1509-1520. [Abstract]; Pentostatin (Deoxycoformycin; dCF) (100 μM; 12-24 h) induced a detectable band of active caspase-3 in mouse macrophages RAW 264.7 treated with dAdo.

: Pentostatin purchased from MedChemExpress. Usage Cited in: Virulence. 2018;9(1):1509-1520. [Abstract]; Pentostatin (Deoxycoformycin; dCF) (100 μM; 12 h) induced a detectable band of active caspase-3 in mouse macrophages RAW 264.7 treated with tNT+dAMP.

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Actividad biológica
Protocolo
Pureza y Documentación
Referencias
Revisión del cliente

Descripciòn

Pentostatin (CI-825; Deoxycoformycin) is an irreversible inhibitor of adenosine deaminase with K_i of 2.5 pM.

IC₅₀ & Target

Ki: 2.5 pM (adenosine deaminase)

Cellular Effect

Cell Line	Type	Value	Description	References
L1210	IC₅₀	0.25 μM Compound: pentostatin	Compound was evaluated for the cytotoxicity against L1210 cells in presence of coformycin Compound was evaluated for the cytotoxicity against L1210 cells in presence of coformycin	[PMID: 3489838]
L1210	IC₅₀	1.9 μM Compound: pentostatin	Compound was evaluated for the cytotoxicity against L1210 cells in absence of coformycin Compound was evaluated for the cytotoxicity against L1210 cells in absence of coformycin	[PMID: 3489838]

In Vivo

In ECP and Pentostatin (CI-825; Deoxycoformycin; 4 mg/m², i.v.) treatment, all dogs develop granulocytopenia with <500 granulocytes/μL from day 4. Thrombocytopenia (<20,000 platelets/μL) occurs from day 7 after HCT with nadirs of 3000 to 14000 platelets/μL^[1]. Pentostatin (2 mg/kg) in combination with cordycepin (2 mg/kg) is 100% effective in the T. evansi-infected mice. There is an increase in levels of some biochemical parameters, especially on liver enzymes, which are accompanied by histological lesions in the liver and kidneys. Pentostatin individually has no curative effect on infected groups^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ensayo clínico

Peso molecular

268.27

Fòrmula

C₁₁H₁₆N₄O₄

No. CAS

53910-25-1

Appearance

Solid

Color

White to off-white

SMILES

O[C@H]1C(N=CN2[C@H](O[C@@H]3CO)C[C@@H]3O)=C2NC=NC1

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvente y solubilidad

In Vitro:

H₂O : 100 mg/mL (372.75 mM; Need ultrasonic)

DMSO : ≥ 50 mg/mL (186.38 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7275 mL	18.6377 mL	37.2755 mL
5 mM	0.7455 mL	3.7275 mL	7.4551 mL
10 mM	0.3728 mL	1.8638 mL	3.7275 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (7.75 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: Saline
Solubility: 100 mg/mL (372.75 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Pureza y Documentación

Purity: 99.93%

Select Batch:

Ficha de datos (280 KB) SDS (479 KB)

COA (255 KB) HNMR (214 KB) LCMS (124 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Bethge WA, et al. Extracorporeal photopheresis combined with pentostatin in the conditioning regimen for canine hematopoietic cell transplantation does not prevent GVHD. Bone Marrow Transplant. 2014 Sep;49(9):1198-204. [Content Brief]

[2]. Dalla Rosa L, et al. Cordycepin (3'-deoxyadenosine) pentostatin (deoxycoformycin) combination treatment of mice experimentally infected with Trypanosoma evansi. Parasitology. 2013 Apr;140(5):663-71. [Content Brief]

Administraciòn de animales
^[1]

All recipient dogs are conditioned for transplantation by 920 cGy TBI at 7 cGy/minute using a linear accelerator. Dogs in group A1receive ECP administered on days −2 and −1 with TBI on day 0 and dogs in group A2receive ECP on days −6 and −5, intravenous (IV) infusion of pentostatin at a dose of 4 mg/m² on days −4 and −3, and TBI on day 0. Donor marrow cells from DLA-nonidentical donors are aspirated under general anesthesia through needles inserted into humeri and femora and stored in heparinized tissue culture medium at 4°C for no more than 6 hours. Within 4 hours of TBI, harvested marrow cells are infused IV into recipients at a median dose of 2.9 (range, 1.9 to 6.1) ×10⁸ total nucleated cells (TNC)/kg. The day of marrow grafting is designated as day 0. In addition to marrow graft, recipients are given IV infusions of peripheral blood buffy coat cells obtained by leukapheresis from the marrow donor on days 1 and 2, at a median dose of 2.3 (range, 1.2 to 6.9) ×10⁸ TNC/kg to ensure consistent hematopoietic engraftment. MTX, at a dose of 0.4 mg/kg intravenously is used as postgrafting immunosuppression and administered on days +1, +3, +6 and +11, then weekly thereafter until day 102.

MCE no ha confirmado la precisión de estos métodos independientemente. Son solo para referencia.

Referencias

[1]. Bethge WA, et al. Extracorporeal photopheresis combined with pentostatin in the conditioning regimen for canine hematopoietic cell transplantation does not prevent GVHD. Bone Marrow Transplant. 2014 Sep;49(9):1198-204. [Content Brief]

[2]. Dalla Rosa L, et al. Cordycepin (3'-deoxyadenosine) pentostatin (deoxycoformycin) combination treatment of mice experimentally infected with Trypanosoma evansi. Parasitology. 2013 Apr;140(5):663-71. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	3.7275 mL	18.6377 mL	37.2755 mL	93.1887 mL
	5 mM	0.7455 mL	3.7275 mL	7.4551 mL	18.6377 mL
	10 mM	0.3728 mL	1.8638 mL	3.7275 mL	9.3189 mL
	15 mM	0.2485 mL	1.2425 mL	2.4850 mL	6.2126 mL
	20 mM	0.1864 mL	0.9319 mL	1.8638 mL	4.6594 mL
	25 mM	0.1491 mL	0.7455 mL	1.4910 mL	3.7275 mL
	30 mM	0.1243 mL	0.6213 mL	1.2425 mL	3.1063 mL
	40 mM	0.0932 mL	0.4659 mL	0.9319 mL	2.3297 mL
	50 mM	0.0746 mL	0.3728 mL	0.7455 mL	1.8638 mL
	60 mM	0.0621 mL	0.3106 mL	0.6213 mL	1.5531 mL
	80 mM	0.0466 mL	0.2330 mL	0.4659 mL	1.1649 mL
	100 mM	0.0373 mL	0.1864 mL	0.3728 mL	0.9319 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Pentostatin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pentostatin53910-25-1CI-825 DeoxycoformycinCI825CI 825Adenosine DeaminaseAntibioticInhibitorinhibitorinhibit

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Pentostatin (Synonyms: CI-825; Deoxycoformycin)

Revisión del cliente

Other Forms of Pentostatin:

Pentostatin Related Antibodies

10 Publications Citing Use of MCE Pentostatin

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Ver todos los productos específicos de isoformas Antibiotic:

Actividad biológica

Protocolo

Pureza y Documentación

Referencias

Revisión del cliente

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

Pentostatin Related Classifications

Keywords:

Productos vistos recientemente:

Revisión del cliente

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report