1. Neuronal Signaling Metabolic Enzyme/Protease Apoptosis
  2. Monoamine Oxidase COMT Apoptosis Endogenous Metabolite
  3. Rosmarinic acid

ロスマリン酸  (Synonyms: Rosmarinic acid; Labiatenic acid)

製品番号: HY-N0529 純度: 99.73%
COA 取扱説明書 Technical Support

Rosmarinic acid is a widespread phenolic ester compound in the plants. Rosmarinic acid inhibits MAO-A, MAO-B and COMT enzymes with IC50s of 50.1, 184.6 and 26.7 μM, respectively.

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CAS 番号 : 20283-92-5

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10 mM * 1 mL in DMSO
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100 mg $108 在庫あり
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カスタマーレビュー

Based on 17 publication(s) in Google Scholar

Other Forms of Rosmarinic acid:

Top Publications Citing Use of Products

    Rosmarinic acid purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Oct 26:148:157482.  [Abstract]

    Detection of cell viability of senescent HL-1 cells treated with different concentrations of Rosmarinic acid (RA) (1.5625-50 μM) and 10 μM ABT263 for 24 h.

    Rosmarinic acid purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Oct 26:148:157482.  [Abstract]

    The representative graphs of SA-β-gal staining and statistical result of SA-β-gal positive cell number in the NC, NC + Rosmarinic acid (RA) (20 mg/kg; i.g.), DOX and DOX + RA groups.

    Rosmarinic acid purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Oct 26:148:157482.  [Abstract]

    Rosmarinic acid (RA) (400 μM) interacted with the Ywhaq protein in cells via the pull-down assay, followed by Western-blot.

    Rosmarinic acid purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Oct 26:148:157482.  [Abstract]

    Rosmarinic acid (RA) (20 μM, 24 h) treatment and Ywhaq overexpression downregulated the mRNA expression of senescence markers (p21, p53), embryonic cardiac genes (Anp, Bnp), and the fibrosis-related gene Col1a1 compared to DOX-induced HL-1 cells.

    Rosmarinic acid purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Oct 26:148:157482.  [Abstract]

    Rosmarinic acid (RA) (20 mg/kg, 100 mg/kg; i.g.) treatment effectively attenuated DOX-induced p21 expression increase and restored the expression of both Ywhaq and Ywhaz.

    Monoamine Oxidase アイソフォーム固有の製品をすべて表示:

    Endogenous Metabolite アイソフォーム固有の製品をすべて表示:

    • 生物活性

    • プロトコル

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Rosmarinic acid is a widespread phenolic ester compound in the plants. Rosmarinic acid inhibits MAO-A, MAO-B and COMT enzymes with IC50s of 50.1, 184.6 and 26.7 μM, respectively.

    IC50 & Target

    IC50: 50.1 μM (MAO-A), 184.6 μM (MAO-B), 26.7 μM (COMT)[1]

    Cellular Effect
    Cell Line Type Value Description References
    HeLa IC50
    0.96 μM
    Compound: 5
    Cytotoxicity against human HeLa cells assessed as inhibition of cell growth and measured after 72 hrs resazurin reduction assay
    Cytotoxicity against human HeLa cells assessed as inhibition of cell growth and measured after 72 hrs resazurin reduction assay
    [PMID: 36126331]
    HeLa IC50
    16.2 μM
    Compound: 4
    Inhibition of transcription factor AP-1 binding to oligonucleotide containing TPA-responsive element in TPA-activated human HeLa cells after 1 hr by ELISA
    Inhibition of transcription factor AP-1 binding to oligonucleotide containing TPA-responsive element in TPA-activated human HeLa cells after 1 hr by ELISA
    [PMID: 24491635]
    HT-22 EC50
    86 μM
    Compound: 23
    Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay
    Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay
    [PMID: 32991171]
    MOLM-13 IC50
    2.9 μM
    Compound: 19
    Antiproliferative activity against human MOLM13 cells by Cell-Titer Glo assay
    Antiproliferative activity against human MOLM13 cells by Cell-Titer Glo assay
    [PMID: 30370766]
    MOLM-14 IC50
    7.1 μM
    Compound: 19
    Antiproliferative activity against human MOLM14 cells by Cell-Titer Glo assay
    Antiproliferative activity against human MOLM14 cells by Cell-Titer Glo assay
    [PMID: 30370766]
    MT4 CC50
    55 μM
    Compound: rosmarinic acid, 1
    Cytotoxicity against human MT4 cells by MTT method
    Cytotoxicity against human MT4 cells by MTT method
    [PMID: 18351727]
    MT4 EC50
    >55 μM
    Compound: rosmarinic acid, 1
    Antiviral activity against HIV1 3B infected in human MT4 cells assessed as reduction of virus-induced cytopathogenicity by MTT assay
    Antiviral activity against HIV1 3B infected in human MT4 cells assessed as reduction of virus-induced cytopathogenicity by MTT assay
    [PMID: 18351727]
    MV4-11 IC50
    >10 μM
    Compound: 19
    Antiproliferative activity against human MV4-11 cells by Cell-Titer Glo assay
    Antiproliferative activity against human MV4-11 cells by Cell-Titer Glo assay
    [PMID: 30370766]
    PC-12 EC50
    57.2 μM
    Compound: rosmarinic acid
    Inhibition of amyloid beta (1 to 42 residues) (unknown origin) aggregate-induced toxicity in rat PC12 cells assessed as cell viability at 4 to 100 ug/ml pre-incubated for 24 hrs with Abeta ( 1 to 42) followed by compound-Abeta (1 to 42) mixture addition t
    Inhibition of amyloid beta (1 to 42 residues) (unknown origin) aggregate-induced toxicity in rat PC12 cells assessed as cell viability at 4 to 100 ug/ml pre-incubated for 24 hrs with Abeta ( 1 to 42) followed by compound-Abeta (1 to 42) mixture addition t
    [PMID: 26517152]
    PC-12 EC50
    68.1 μM
    Compound: rosmarinic acid
    Inhibition of amyloid beta (25 to 35 residues) (unknown origin) aggregate-induced toxicity in rat PC12 cells preincubated for 1 hr followed by amyloid beta challenge measured after 24 hrs by MTT assay
    Inhibition of amyloid beta (25 to 35 residues) (unknown origin) aggregate-induced toxicity in rat PC12 cells preincubated for 1 hr followed by amyloid beta challenge measured after 24 hrs by MTT assay
    [PMID: 26517152]
    PC-3 IC50
    >10 μM
    Compound: 5
    Cytotoxicity against human PC-3 cells assessed as inhibition of cell growth and measured after 72 hrs resazurin reduction assay
    Cytotoxicity against human PC-3 cells assessed as inhibition of cell growth and measured after 72 hrs resazurin reduction assay
    [PMID: 36126331]
    RAW264.7 IC50
    34.2 μM
    Compound: 25
    Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as decrease in PGE2 production preincubated for 1 hr followed by LPS stimulation and measured after 24 hrs by ELISA
    Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as decrease in PGE2 production preincubated for 1 hr followed by LPS stimulation and measured after 24 hrs by ELISA
    [PMID: 31747281]
    Sf9 IC50
    1.3 μM
    Compound: Rosmarinic acid
    Inhibition of human Fyn expressed in Sf9 cells after 20 mins by ELISA in presence of 1 umol/L ATP
    Inhibition of human Fyn expressed in Sf9 cells after 20 mins by ELISA in presence of 1 umol/L ATP
    [PMID: 17315853]
    Sf9 IC50
    17 μM
    Compound: Rosmarinic acid
    Inhibition of human Fyn expressed in Sf9 cells after 20 mins by ELISA in presence of 100 umol/L ATP
    Inhibition of human Fyn expressed in Sf9 cells after 20 mins by ELISA in presence of 100 umol/L ATP
    [PMID: 17315853]
    Sf9 IC50
    3.6 μM
    Compound: Rosmarinic acid
    Inhibition of human Fyn expressed in Sf9 cells after 20 mins by ELISA in presence of 10 umol/L ATP
    Inhibition of human Fyn expressed in Sf9 cells after 20 mins by ELISA in presence of 10 umol/L ATP
    [PMID: 17315853]
    Sf9 IC50
    36 μM
    Compound: Rosmarinic acid
    Inhibition of human Fyn expressed in Sf9 cells after 1 min by ELISA in presence of 1 umol/L ATP
    Inhibition of human Fyn expressed in Sf9 cells after 1 min by ELISA in presence of 1 umol/L ATP
    [PMID: 17315853]
    Sf9 IC50
    63 μM
    Compound: Rosmarinic acid
    Inhibition of human Fyn expressed in Sf9 cells after 1 min by ELISA in presence of 100 umol/L ATP
    Inhibition of human Fyn expressed in Sf9 cells after 1 min by ELISA in presence of 100 umol/L ATP
    [PMID: 17315853]
    Sf9 IC50
    74 μM
    Compound: Rosmarinic acid
    Inhibition of human Fyn expressed in Sf9 cells after 1 min by ELISA in presence of 10 umol/L ATP
    Inhibition of human Fyn expressed in Sf9 cells after 1 min by ELISA in presence of 10 umol/L ATP
    [PMID: 17315853]
    SH-SY5Y IC50
    >10 μM
    Compound: 5
    Cytotoxicity against human SH-SY5Y cells assessed as inhibition of cell growth and measured after 72 hrs resazurin reduction assay
    Cytotoxicity against human SH-SY5Y cells assessed as inhibition of cell growth and measured after 72 hrs resazurin reduction assay
    [PMID: 36126331]
    体外実験

    Rosmarinic acid (RA) shows an in vitro multifunctional profile characterized by antioxidant effects, and monoamine oxidases (MAO-A and MAO-B) and catechol-O-methyl transferase (COMT) inhibition. Rosmarinic acid shows antioxidant effects against hydroxyl (HO-) and nitric oxide (NO) radicals (IC50 of 29.4 and 140 μM, respectively), and inhibition of lipid peroxidation (IC50 of 19.6 μM)[1]. Rosmarinic acid (RA) exerts a significant cytoprotective effect by scavenging intracellular ROS induced by UVB. In H2O2-treated cells, 2.5 μM Rosmarinic acid scavenges 60% of intracellular ROS compared to 77% of intracellular ROS scavenging effect in N-acetyl-L-cysteine (NAC)[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    体内実験

    Rosmarinic acid (RA) is a widespread phenolic ester compound in the plants, particularly those in the Labiatae family of herbs, such as Rosmarinus officinali, Salvia miltiorrhiza, and Prunella vulgaris. Rosmarinic acid suppresses colonic inflammation in dextran sulphate sodium (DSS)-induced mice via dual inhibition of NF-κB and STAT3 activation. In the DSS-induced colitis model, Treatment with Rosmarinic acid (30, 60 mg/kg, p.o.) markedly attenuates the production of cytokines[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    臨床実験
    分子量

    360.31

    分子式

    C18H16O8

    CAS 番号
    Appearance

    Solid

    Color

    Off-white to light brown

    SMILES

    OC([C@H](OC(/C=C/C1=CC(O)=C(O)C=C1)=O)CC2=CC(O)=C(O)C=C2)=O

    Structure Classification
    Initial Source
    輸送条件

    Room temperature in continental US; may vary elsewhere.

    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 50 mg/mL (138.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 2 mg/mL (5.55 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.7754 mL 13.8769 mL 27.7539 mL
    5 mM 0.5551 mL 2.7754 mL 5.5508 mL
    10 mM 0.2775 mL 1.3877 mL 2.7754 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

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    一般には略語で表示されます:C1V1 = C2V2

    濃度 (開始)

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    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (5.77 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (5.77 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    +
    %
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    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.73%

    参考文献
    細胞実験
    [2]

    Human keratinocytes (HaCaT cells) are treated with Rosmarinic acid (0.625, 1.25, 2.5, or 5 μM) and exposed to UVB radiation 1 h later. They are then incubated at 37°C for 48 h. At this time, MTT is added to each well to obtain a total reaction volume of 200 μL. After 4 h incubation, the supernatant is removed by aspiration. The formazan crystals in each well are dissolved in dimethyl sulfoxide (DMSO; 150 μL), and the absorbance at 540 nm is measured on a scanning multi-well spectrophotometer[2].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。

    動物実験
    [3]

    Mice[3]
    Experimental colitis is induced by giving mice drinking water ad libitum containing 5% (w/v) DSS for 7 days. Mice of each of the groups are monitored carefully every day to confirm that they have consumed an approximately equal volume of water containing DSS. For each experiment, the mice are divided into five experimental groups (n = 10/group). The first group is kept as the vehicle-treated control, and the second group is given drinking water with DSS only during the experimental period. The other three groups consist of mice receiving 5% DSS who are administrated 5-ASA (75 mg/kg/day p.o.) or Rosmarinic acid (30 or 60 mg/kg/day p.o.) daily for 7 days. Control groups are given the vehicle daily for 7 days as appropriate. Administration of each drug is initiated simultaneously with the DSS treatment.

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。

    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 2.7754 mL 13.8769 mL 27.7539 mL 69.3847 mL
    5 mM 0.5551 mL 2.7754 mL 5.5508 mL 13.8769 mL
    DMSO 10 mM 0.2775 mL 1.3877 mL 2.7754 mL 6.9385 mL
    15 mM 0.1850 mL 0.9251 mL 1.8503 mL 4.6256 mL
    20 mM 0.1388 mL 0.6938 mL 1.3877 mL 3.4692 mL
    25 mM 0.1110 mL 0.5551 mL 1.1102 mL 2.7754 mL
    30 mM 0.0925 mL 0.4626 mL 0.9251 mL 2.3128 mL
    40 mM 0.0694 mL 0.3469 mL 0.6938 mL 1.7346 mL
    50 mM 0.0555 mL 0.2775 mL 0.5551 mL 1.3877 mL
    60 mM 0.0463 mL 0.2313 mL 0.4626 mL 1.1564 mL
    80 mM 0.0347 mL 0.1735 mL 0.3469 mL 0.8673 mL
    100 mM 0.0278 mL 0.1388 mL 0.2775 mL 0.6938 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    一般には略語で表示されます:C1V1 = C2V2

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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    製品名:
    Rosmarinic acid
    製品番号:
    HY-N0529
    数量:
    MCE 日本正規代理店: