2. Cell Cycle/DNA Damage Epigenetics Autophagy Apoptosis
  3. HDAC Autophagy Apoptosis
  4. Sodium 4-phenylbutyrate

Sodium 4-phenylbutyrate  (Synonyms: 4-PBA (sodium); 4-Phenylbutyric acid (sodium); Benzenebutyric acid (sodium))

製品番号: HY-15654 純度: 99.86%
COA 取扱説明書 Technical Support

Sodium 4-phenylbutyrate (4-PBA sodium) is an inhibitor of HDAC and endoplasmic reticulum (ER) stress, used in cancer and infection research.

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CAS 番号 : 1716-12-7

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100 mg $66 在庫あり
200 mg $106 在庫あり
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カスタマーレビュー

Based on 239 publication(s) in Google Scholar

Other Forms of Sodium 4-phenylbutyrate:

Sodium 4-phenylbutyrate 関連抗体

Top Publications Citing Use of Products

顧客検証

WB
Flow Cytometry
Cell Proliferation/Viability Assay
Cell Migration/Invasion Assay

    Sodium 4-phenylbutyrate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Aug 29;16(1):8074.  [Abstract]

    Western blot of the protein expression of ATF4, CHOP and the osteogenic marker BMP2 in the aortas from the male mice subjected to VitD3-induced vascular calcification, with or without treatment of 4PBA (20 mg/kg/day, ip).

    Sodium 4-phenylbutyrate purchased from MedChemExpress. Usage Cited in: Nat Cell Biol. 2024 Nov;26(11):1892-1902.  [Abstract]

    Percentages and numbers of CD45.1+CD8+ OT-I Teff cells in the spleens of mice treated with 4PBA (200 mg/kg, ip) or PBS (n = 8 mice per group).

    Sodium 4-phenylbutyrate purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2023 Apr 14:27:257-270.

    The protein levels of key molecules of ER stress and apoptosis in HG-induced fibroblasts after NET (1000 ng/mL, 24 h) treatment with or without pre-incubation for 1.5 h with 4-PBA (5 μM) or DNase I (2 U/mL).

    Sodium 4-phenylbutyrate purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2023 Apr 14:27:257-270.

    Edu assays were used to assess the proliferation of HG-induced fibroblasts after 24 hours of treatment with 4-PBA (5 μM) or DNase I (2 U/mL). Edu, red;DAPI, blue.

    Sodium 4-phenylbutyrate purchased from MedChemExpress. Usage Cited in: Bioact Mater. 2023 Apr 14:27:257-270.

    Migration ability of HG-induced fibroblasts was analyzed by transwell migration assay following the indicated treatment with 4-PBA (5 μM) or DNase I (2 U/mL) for 24 h.

    Sodium 4-phenylbutyrate purchased from MedChemExpress. Usage Cited in: Autophagy. 2021 Nov;17(11):3592-3606.  [Abstract]

    Cells are treated with 20 μM Cannabidiol for 24 h with or without pretreatment with 4-PBA (1 mM for 30 min); expression levels of ER stress and mitophagy related proteins are analyzed by western blotting.

    HDAC アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

    • 生物活性

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    • 純度とドキュメンテーション

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    製品説明

    Sodium 4-phenylbutyrate (4-PBA sodium) is an inhibitor of HDAC and endoplasmic reticulum (ER) stress, used in cancer and infection research[1].

    IC50 & Target[1]

    HDAC

     

    体外実験

    Sodium 4-phenylbutyrate (Sodium phenylbutyrate) is an inhibitor of HDAC, inhibits the growth of NSCLC Cell Lines at 2 mM. Sodium 4-phenylbutyrate in combination with ciglitizone results in enhanced growth arrest of cancer cells[1].
    Sodium 4-phenylbutyrate (Sodium phenylbutyrate; 0-5 mM) inhibits ASFV infection in a dose-dependent manner. Sodium 4-phenylbutyrate also inhibits the ASFV late protein synthesis and disrupts the virus-induced H3K9/K14 hypoacetylation status. Sodium 4-phenylbutyrate and Enrofloxacin act synergistically to abolish ASFV replication[2].
    Addition of Bafilomycin A1 results in accumulation of LC3II, whereas Benzenebutyric acid (4-PBA) substantially reduces this accumulation. LPS decreases the level of p62, whereas Benzenebutyric acid reverses this decrease upon LPS stimulation for 48 h. The percentage of cells with LPS-induced AVOs is increased at 48 h, whereas Benzenebutyric acid significantly reduces this percentage. Specifically, the percentage of cells with AVOs decreases from 61.6% to 53.1% upon Benzenebutyric acid treatment, supporting that Benzenebutyric acid inhibits LPS-induced autophagy. As a positive control for autophagy inhibition, bafilomycin A1 is used. The percentage of cells with LPS-induced AVOs is reduced by bafilomycin A1 treatment. The decreased OC area and fusion index observed after Benzenebutyric acid treatment are not observed with knockdown of ATG7. Inhibition of NF-κB using BAY 11-7082 and JSH23 reduce the LC3 II level upon LPS stimulation and completely abolish the inhibitory effect of Benzenebutyric acid on LPS-induced effects[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Sodium 4-phenylbutyrate 関連抗体
    体内実験

    LPS induces significant bone loss and decreases bone mineral density (BMD), bone volume (BV/TV), and trabecular thickness (Tb. Th) compared with PBS alone, whereas trabecular space (Tb. Sp.) is increased. Sodium 4-phenylbutyrate (Sodium phenylbutyrate) attenuates LPS-induced bone loss. Treatment with Sodium 4-phenylbutyrate increases BMD, BV/TV, and Tb. Th. compared with LPS alone, in addition to decreasing the enlargement of Tb. Sp., but no change is observed when mice are treated with Sodium 4-phenylbutyrate alone. OC.S/BS as assessed by TRAP staining is also significantly reduced when Sodium 4-phenylbutyrate is administered to LPS-treated mice. However, OC.N/BS tends to decrease, although not with statistical significance, when mice are treated with Sodium 4-phenylbutyrate and LPS. These results indicate that the effect of Sodium 4-phenylbutyrate on OC from LPS-treated mice is to reduce its size rather than number. Consistent with these findings, a marker of bone resorption in vivo, serum CTX-1 which is elevated by LPS treatment is decreased when Sodium 4-phenylbutyrate administered to LPS-injected mice. However, co-treatment with Sodium 4-phenylbutyrate do not significantly affect the levels of serum ALP and osteocalcin, 2 markers of bone formation in vivo, compared with LPS alone. Sodium 4-phenylbutyrate also reduces the LPS-induced rise in serum MCP-1, indicating that Sodium 4-phenylbutyrate decreases systemic inflammation induced by LPS[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    臨床実験
    分子量

    186.18

    分子式

    C10H11NaO2
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(O[Na])CCCC1=CC=CC=C1
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
    溶剤 & 溶解度
    体外: 

    H2O : 100 mg/mL (537.11 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.3711 mL 26.8557 mL 53.7115 mL
    5 mM 1.0742 mL 5.3711 mL 10.7423 mL
    10 mM 0.5371 mL 2.6856 mL 5.3711 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    一般には略語で表示されます：C1V1 = C2V2

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    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (537.11 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
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    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.86%

    COA (244 KB) HNMR (218 KB) LCMS (96 KB)

    取扱説明書 (2659 KB)
    参考文献
    細胞実験
    [1]

    Briefly, viable cells, as judged by trypan blue dye exclusion, are seeded at a density of 4 × 104 cells/mL in 60-mm dishes in RPMI 1640 with 10% fetal bovine serum and 0.35% agarose on a base layer of 0.7% agarose. DMSO, TSA, or PB is added to both bottom and top agarose layers. Assays are performed in triplicate on at least three separate occasions, and colonies are counted at 10-14 days[1].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    動物実験
    [3]

    Mice[3]
    Female 10-week-old C57BL/6J mice are housed in the pathogen-free animal facility of IRC. Animals are randomized into the following 4 groups: vehicle control (n=5), vehicle+Benzenebutyric acid (n=6), LPS (n=6), and LPS+Benzenebutyric acid (n=6). Mice are treated with LPS in 200 μL phosphate-buffered saline (PBS) once a week (5 mg/kg, i.p.) for 3 weeks. Sodium 4-phenylbutyrate (Benzenebutyric acid) solution is prepared by titrating equimolecular amounts of Benzenebutyric acid and sodium hydroxide to reach pH 7.4; mice are injected daily intraperitoneally in 200 μL PBS (or with PBS as a vehicle) at a dose of 240 mg/kg for 3 weeks. Mice are sacrificed by CO2 asphyxiation. To determine the bone mineral density (BMD) and microarchitecture of the long bone, the right femur is scanned. Scans are performed with an effective detector pixel size of 6.9 μm and a threshold of 77-255 mg/cc. Trabecular bone is analyzed in a region 1.6 mm in length and located 0.1 mm below the distal femur growth plate[3].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 5.3711 mL 26.8557 mL 53.7115 mL 134.2787 mL
    5 mM 1.0742 mL 5.3711 mL 10.7423 mL 26.8557 mL
    10 mM 0.5371 mL 2.6856 mL 5.3711 mL 13.4279 mL
    15 mM 0.3581 mL 1.7904 mL 3.5808 mL 8.9519 mL
    20 mM 0.2686 mL 1.3428 mL 2.6856 mL 6.7139 mL
    25 mM 0.2148 mL 1.0742 mL 2.1485 mL 5.3711 mL
    30 mM 0.1790 mL 0.8952 mL 1.7904 mL 4.4760 mL
    40 mM 0.1343 mL 0.6714 mL 1.3428 mL 3.3570 mL
    50 mM 0.1074 mL 0.5371 mL 1.0742 mL 2.6856 mL
    60 mM 0.0895 mL 0.4476 mL 0.8952 mL 2.2380 mL
    80 mM 0.0671 mL 0.3357 mL 0.6714 mL 1.6785 mL
    100 mM 0.0537 mL 0.2686 mL 0.5371 mL 1.3428 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Sodium 4-phenylbutyrate Related Classifications

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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Sodium 4-phenylbutyrate1716-12-74-PBA4-Phenylbutyric acidBenzenebutyric acidHDACAutophagyApoptosisHistone deacetylasesInhibitorinhibitorinhibit

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