2. Metabolic Enzyme/Protease Anti-infection
  3. MMP SARS-CoV
  4. TAPI-2

TAPI-2  (Synonyms: TNF Protease Inhibitor 2)

Art. -Nr.: HY-100211 Reinheit: 97.41%
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TAPI-2 (TNF Protease Inhibitor 2) is a broad-spectrum inhibitor of matrix metalloprotease (MMP), tumour necrosis factorα-converting enzyme (TACE) and a disintegrin and metalloproteinase (ADAM), with an IC50 of 20 μM for MMP. TAPI-2 blocks the entry of infectious SARS-CoV.

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CAS. Nr. : 187034-31-7

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Solution
10 mM * 1 mL in Ethanol Auf Lager
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Based on 5 publication(s) in Google Scholar

Other Forms of TAPI-2:

TAPI-2 Related Antibodies

Top Publications Citing Use of Products

    TAPI-2 purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2020 Jul 29;39(1):145.  [Abstract]

    Representative images of HE staining of 3D cultures of Huh-7 and CD147 knockout cells treated with or without TAPI-2.

    Alle MMP Isoform-spezifische Produkte anzeigen:

    Alle Isoformen anzeigen
    MMP-1 MMP-2 MMP-3 MMP-8 MMP-9 MMP-13 MMP-14 MMP-17 ADAM10 ADAM17 MMP-7 MMP-12 MMP-10 MMP ADAM8 MMP-19

    • Biologische Aktivität

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    Beschreibung

    TAPI-2 (TNF Protease Inhibitor 2) is a broad-spectrum inhibitor of matrix metalloprotease (MMP), tumour necrosis factorα-converting enzyme (TACE) and a disintegrin and metalloproteinase (ADAM), with an IC50 of 20 μM for MMP[1]. TAPI-2 blocks the entry of infectious SARS-CoV[2].

    IC50 & Target[1]

    MMP

    20 μM (IC50)

    In Vitro

    The hydroxamate-based metalloprotease inhibitor TAPI-2 bounds to hmeprin with inhibition constants IC50 20±10 μM for hmeprin β subunit and 1.5±0.27 nM for hmeprin α subunit. Generally, hmeprin α is inhibited more strongly than the β subunit[1]. Without affecting ADAM17 expression, TAPI-2 dramatically decreases the protein levels of NICD and its downstream target HES-1 in both HCP-1 and HT29 cells. Moreover, treating cells with TAPI-2 significantly decreases the CSC phenotype by -50% in both CRC cell lines. The dose-dependent effects of TAPI-2 on the sphere formation and protein levels of NICD and HES-1 confirm that the concentration used (20 μM) is within the effective dose range of TAPI-2 (5-40 μM)[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    TAPI-2 Related Antibodies
    Molekulargewicht

    415.53

    Formel

    C19H37N5O5
    CAS. Nr.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    CC(C)(C)[C@@H](C(N[C@H](C(NCCN)=O)C)=O)NC(C(CC(NO)=O)CC(C)C)=O
    Versand

    Room temperature in continental US; may vary elsewhere.
    Speicherung

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Lösungsmittel & Löslichkeit
    In Vitro: 

    Ethanol : ≥ 100 mg/mL (240.66 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4066 mL 12.0328 mL 24.0657 mL
    5 mM 0.4813 mL 2.4066 mL 4.8131 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molaritätsrechner

    • Verdünnungsrechner

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

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    (per animal)

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    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Reinheit & Dokumentation

    Purity: 97.41%

    SDS (252 KB)

    COA (244 KB) HNMR (438 KB) RP-HPLC (244 KB) MS (71 KB)

    Handling Instructions (2659 KB)
    Verweise
    Zellassay
    [3]

    TAPI-2 is dissolved in DMSO and diluted with appropriate medium before use. All experiments are performed using 20 μM TAPI-2. Cells are cultured with or without TAPI-2 for 48 hours and then seeded at 3,000 cells per well in 96-well plates. After pretreatment, increasing doses of 5-fluorouracil (5-FU) that are relevant to the recommended clinical dose (up to 2 μg/mL) are added, with or without TAPI-2, for 72 hours. Cell viability is assessed by adding MTT substrate (0.25% in phosphate-buffered saline [PBS]) in growth medium (1:5 dilution) to cells for 1 hour at 37°C. The cells are ished with PBS, and 100 μL of dimethyl sulfoxideis added. Optical density is measured at 570 nM, and relative MTT is presented as a percentage of control[3].

    MCE hat die Genauigkeit dieser Methoden nicht unabhängig bestätigt. Sie dienen nur als Referenz.
    Verweise

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Ethanol 1 mM 2.4066 mL 12.0328 mL 24.0657 mL 60.1641 mL
    5 mM 0.4813 mL 2.4066 mL 4.8131 mL 12.0328 mL
    10 mM 0.2407 mL 1.2033 mL 2.4066 mL 6.0164 mL
    15 mM 0.1604 mL 0.8022 mL 1.6044 mL 4.0109 mL
    20 mM 0.1203 mL 0.6016 mL 1.2033 mL 3.0082 mL
    25 mM 0.0963 mL 0.4813 mL 0.9626 mL 2.4066 mL
    30 mM 0.0802 mL 0.4011 mL 0.8022 mL 2.0055 mL
    40 mM 0.0602 mL 0.3008 mL 0.6016 mL 1.5041 mL
    50 mM 0.0481 mL 0.2407 mL 0.4813 mL 1.2033 mL
    60 mM 0.0401 mL 0.2005 mL 0.4011 mL 1.0027 mL
    80 mM 0.0301 mL 0.1504 mL 0.3008 mL 0.7521 mL
    100 mM 0.0241 mL 0.1203 mL 0.2407 mL 0.6016 mL
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    TAPI-2 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    TAPI-2187034-31-7TNF Protease Inhibitor 2TAPI2TAPI 2TNF Protease Inhibitor2TNF Protease Inhibitor-2MMPSARS-CoVMatrix metalloproteinasesSARS coronavirusInhibitorinhibitorinhibit

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