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(-)-Epigallocatechin Gallate (Synonyms: EGCG; Epigallocatechol Gallate)

Cat. No.: HY-13653 Purity: 99.91%
Handling Instructions

(-)-Epigallocatechin Gallate is a tea flavonoid with potent antioxidant, antiinflammatory, and anticarcinogenic properties. (-)-Epigallocatechin Gallate is reported to inhibit EGFR signaling and thereby exert anticancer effects.

For research use only. We do not sell to patients.

(-)-Epigallocatechin Gallate Chemical Structure

(-)-Epigallocatechin Gallate Chemical Structure

CAS No. : 989-51-5

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
50 mg USD 50 In-stock
Estimated Time of Arrival: December 31
100 mg USD 65 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Other Forms of (-)-Epigallocatechin Gallate:

Top Publications Citing Use of Products

    (-)-Epigallocatechin Gallate purchased from MCE. Usage Cited in: Oncol Lett. 2017 Nov;14(5):6314-6320.

    Effects of EGCG on the levels of SHP 1 and p p38α protein expres¬sion in NB4 cells.
    • Biological Activity

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    • Technical Information

    • Purity & Documentation

    • References

    Description

    (-)-Epigallocatechin Gallate is a tea flavonoid with potent antioxidant, antiinflammatory, and anticarcinogenic properties. (-)-Epigallocatechin Gallate is reported to inhibit EGFR signaling and thereby exert anticancer effects.

    IC50 & Target

    EGFR

     

    HER2

     

    HER3

     

    In Vitro

    (-)-Epigallocatechin Gallate (EGCG) inhibits activation EGFR, HER2 and HER3 in the SW837 human colon cancer cell line. (-)-Epigallocatechin Gallate (10 μM) also inhibits cell growth, suppresses activation of EGFR, HER2, and HER3, and causes decrease in the levels of COX-2 and Bcl-xL proteins, and apoptosis after treatment for 96 h[1]. (-)-Epigallocatechin Gallate (0-35 μg/mL) inhibits the proliferation of colorectal cancer cells. (-)-Epigallocatechin Gallate (35 μg/mL) induces apoptosis of colorectal cancer cells[2]. (-)-Epigallocatechin Gallate (EGCG; 50, 75 and 100 μM) dose-dependently inhibits the growth of HepG2 cells, and induces apoptosis in HepG2 cells[3].

    In Vivo

    (-)-Epigallocatechin Gallate (5, 10, and 20 mg/kg, p.o.) inhibits the growth of orthotopic colorectal cancer cells in mice[2].

    Clinical Trial
    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 30 mg/mL (65.45 mM)

    H2O : 20 mg/mL (43.63 mM; Need ultrasonic)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1816 mL 10.9082 mL 21.8164 mL
    5 mM 0.4363 mL 2.1816 mL 4.3633 mL
    10 mM 0.2182 mL 1.0908 mL 2.1816 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Cell Assay
    [2]

    LoVo, SW480, HCT-8, and HT-29 cells are seeded in 96-well plates at a concentration of 5×103 cells; each cell line is totally seeded in the 12 wells. Complete medium is added to the wells, up to 200 μL; the medium contains 0 μg/mL, 10 μg/mL, 20 μg/mL, and 35 μg/mL of (-)-Epigallocatechin Gallate. The inhibition rate=[1 - (absorbance of (-)-Epigallocatechin Gallate group - absorbance of control group)/(absorbance of control group - absorbance of blank control group)] × 100[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [2]

    Mice[2]
    At 2 weeks postsurgery, 39 out of the 40 nude mice presented with tumors. Based on the volume of the tumors, the 39 mice with tumors are divided into four groups: a control group (n=9); a group that receives 5 mg/kg of (-)-Epigallocatechin Gallate (n=10); a group that receives 10 mg/kg of (-)-Epigallocatechin Gallate (n=10); and a group that receives 20 mg/kg of (-)-Epigallocatechin Gallate (n=10). In the therapeutic groups, (-)-Epigallocatechin Gallate is administrated intragastrically, and in the control group, 100 uL of physiological saline is administrated intragastrically, once daily for 14 days. After the treatment of the mice with (-)-Epigallocatechin Gallate for 4 weeks, the growth and metastasis of the primary tumors are continuously monitored using a fluorescent imaging system. After 4 weeks, the primary tumors are weighed and immediately put into liquid nitrogen (−196°C) and 2 to 3 hours later, these specimens are stored at −80°C. In addition, the other parts of the primary tumor and metastases are fixed in 4% formaldehyde[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    458.37

    Formula

    C₂₂H₁₈O₁₁

    CAS No.

    989-51-5

    SMILES

    O=C(O[[email protected]]1[[email protected]@H](C2=CC(O)=C(O)C(O)=C2)OC3=CC(O)=CC(O)=C3C1)C4=CC(O)=C(O)C(O)=C4

    Shipping

    Room temperature in continental US; may vary elsewhere

    Purity: 99.91%

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    Product Name:
    (-)-Epigallocatechin Gallate
    Cat. No.:
    HY-13653
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    (-)-Epigallocatechin Gallate

    Cat. No.: HY-13653