1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. (-)-Epigallocatechin Gallate

(-)-Epigallocatechin Gallate (Synonyms: EGCG; Epigallocatechol Gallate)

Cat. No.: HY-13653 Purity: 99.87%
Handling Instructions

(-)-Epigallocatechin Gallate (EGCG) is a major polyphenol in green tea, which can inhibit cell proliferation and induce cell apoptosis. (-)-Epigallocatechin Gallate inhibits glutamate dehydrogenase 1/2 (GDH1/2, GLUD1/2) activity. (-)-Epigallocatechin Gallate has a potent anticancer, antioxidant and anti-inflammatory properties against various types of cancers such as colorectal cancer, myeloid leukemia, thyroid carcinoma.

For research use only. We do not sell to patients.

(-)-Epigallocatechin Gallate Chemical Structure

(-)-Epigallocatechin Gallate Chemical Structure

CAS No. : 989-51-5

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10 mM * 1 mL in DMSO USD 61 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 23 publication(s) in Google Scholar

Other Forms of (-)-Epigallocatechin Gallate:

Top Publications Citing Use of Products

    (-)-Epigallocatechin Gallate purchased from MCE. Usage Cited in: Oncol Lett. 2017 Nov;14(5):6314-6320.

    Effects of EGCG on the levels of SHP 1 and p p38α protein expres¬sion in NB4 cells.
    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    (-)-Epigallocatechin Gallate (EGCG) is a major polyphenol in green tea, which can inhibit cell proliferation and induce cell apoptosis. (-)-Epigallocatechin Gallate inhibits glutamate dehydrogenase 1/2 (GDH1/2, GLUD1/2) activity. (-)-Epigallocatechin Gallate has a potent anticancer, antioxidant and anti-inflammatory properties against various types of cancers such as colorectal cancer, myeloid leukemia, thyroid carcinoma[1][2][3][4].

    IC50 & Target[1]

    EGFR

     

    HER2

     

    HER3

     

    Human Endogenous Metabolite

     

    In Vitro

    (-)-Epigallocatechin Gallate (EGCG, 10-60 μM) inhibits the growth of FB-2 and WRO cells in a dose-dependent manner[1].
    (-)-Epigallocatechin Gallate (10-60 μM, 0-24 h) reduces cyclin D1 and phosphorylation of AKT and ERK1/2, and increases p21 and p53 expression[1].
    (-)-Epigallocatechin Gallate (10-60 μM, 12 h) reduces cell motility and migration[1].
    (-)-Epigallocatechin Gallate (0-20 μM, 0-20 min approximately) inhibits GLUD1/2 and IDH1 activity in a concentration and time-dependent way (biochemical assays)[2].
    (-)-Epigallocatechin Gallate (0-35 μg/mL, 24-72 h) inhibits the proliferation of colorectal cancer cells (LoVo, SW480, HT-29, HCT-8 cells), increases cell apoptosis and blocks cells at the G0/G1 phase[3].
    (-)-Epigallocatechin Gallate (30 μM, 3-24 h) suppresses the expression of COX-2 and mPGES-1 mRNAs, prostaglandin E2 production in LPS-induced osteoblasts[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Proliferation Assay[1]

    Cell Line: FB-2 and WRO cells (serum-starved for 48h)
    Concentration: 10, 40, 60 μM.
    Incubation Time: 4 days
    Result: Inhibited basal cell proliferation (40% in FB-2 and 35% in WRO) at 10 μM, inhibited cell number (by 68% to 73%) at 40 and 60 μM).

    Western Blot Analysis[1]

    Cell Line: FB-2 cells
    Concentration: 10, 40, 60 μM.
    Incubation Time: 24 h
    Result: Reduced cyclin D1 level, phosphorylation of AKT and ERK1/2.
    Induced the expression of p21 and p53, and E-cadherin, N-cadherin, Vimentin and α5-integrin.

    Cell Migration Assay [1]

    Cell Line: FB-2 and WRO cells (serum-starved for 48h)
    Concentration: 10, 40, 60 μM.
    Incubation Time: 12 h
    Result: Reduced migration activity in FB-2 and WRO cells.

    RT-PCR[4]

    Cell Line: Mouse primary osteoblasts (1 ng/ml LPS-treated)
    Concentration: 30 μM
    Incubation Time: 3, 6, 12, 24 h
    Result: Suppressed the LPS-induced expression of COX-2 and mPGES-1 mRNAs, prostaglandin E2 production.
    In Vivo

    (-)-Epigallocatechin Gallate (Intragastrical administration, 5-20 mg/kg, once daily for 14 days, orthotopic transplant model) decreases tumors growth[3].
    (-)-Epigallocatechin Gallate (Injected into the mouse lower gingiva, a single dose of 0.5 mg/mouse, experimental periodontitis model) decreases inhibits the LPS-induced loss of bone mineral density (BMD)[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Orthotopic transplant BALB/c nude mice model[3]
    Dosage: 5, 10, and 20 mg/kg, once daily for 14 days.
    Administration: Intragastrical administration.
    Result: Inhibited tumors growth with no liver or lung metastases.
    Animal Model: Model of experimental periodontitis, LPS (25 μg/mouse)[4]
    Dosage: 0.5 mg/mouse, a single dose.
    Administration: Injected into the mouse lower gingiva
    Result: Inhibited the LPS-induced loss of bone mineral density (BMD) in mice.
    Clinical Trial
    Molecular Weight

    458.37

    Formula

    C22H18O11

    CAS No.
    SMILES

    O=C(O[[email protected]]1[[email protected]@H](C2=CC(O)=C(O)C(O)=C2)OC3=CC(O)=CC(O)=C3C1)C4=CC(O)=C(O)C(O)=C4

    Structure Classification
    Source
    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 30 mg/mL (65.45 mM)

    H2O : 20 mg/mL (43.63 mM; Need ultrasonic)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1816 mL 10.9082 mL 21.8164 mL
    5 mM 0.4363 mL 2.1816 mL 4.3633 mL
    10 mM 0.2182 mL 1.0908 mL 2.1816 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.08 mg/mL (4.54 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.08 mg/mL (4.54 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.08 mg/mL (4.54 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 99.87%

    References
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    Product Name:
    (-)-Epigallocatechin Gallate
    Cat. No.:
    HY-13653
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