(-)-Gallocatechin gallate
Based on 3 publication(s) in Google Scholar
(-)-Gallocatechin gallate is the polyphenol isolated from tea, with cancer-preventive activities.
For research use only. We do not sell to patients.
- Purity: 99.97%
- CAS No.: 4233-96-9
- Formula: C22H18O11
- Molecular Weight:458.37
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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HY-N0225
(-)-Epigallocatechin
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HY-N0898
Catechin
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HY-N0002
(-)-Epicatechin gallate
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HY-N0521
(-)-Gallocatechin
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HY-13653
(-)-Epigallocatechin Gallate
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HY-N0521A
(+)-Gallocatechin
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HY-N0356
(-)-Catechin gallate
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HY-112735
Hexadimethrine bromide
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HY-66011A
Moxifloxacin
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HY-12885A
2’3’-c-di-AM(PS)2 (Rp,Rp) disodium salt
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HY-N0526
2"-O-Galloylhyperin
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HY-N0522R
(-)-Gallocatechin gallate (Standard)
Publications Citing Use of MedChemExpress (MCE) (-)-Gallocatechin gallate
More
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| 3T3-L1 | IC50 |
25 μM
Compound: GCG, (-)-gallocatechin gallate
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Inhibition of G6PD-mediated NADPH production in mouse 3T3-L1 cells
Inhibition of G6PD-mediated NADPH production in mouse 3T3-L1 cells
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[PMID: 18313308] |
The amount of (-)-Gallocatechin gallate does not differ in leaves of different stages, and the content is relatively low[1]. (-)-gallocatechin gallate in combination with active catechins ((-)-epigallocatechin gallate) has synergistic effects on the induction of apoptosis and inhibition of cell growth for PC-9 cells. (-)-gallocatechin gallate shows inhibitory effect on α-Glucosidase and DPPH, with IC50s of 30.2 μM and 12.2 μg/mL[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 4233-96-9
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Appearance Solid
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Molecular Weight 458.37
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Formula C22H18O11
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Color White to off-white
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SMILES
O=C(O[C@H]1[C@H](C2=CC(O)=C(O)C(O)=C2)OC3=CC(O)=CC(O)=C3C1)C4=CC(O)=C(O)C(O)=C4
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Synonyms
(-)-Gallocatechol gallate
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (3)
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Journal Impact Factor
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Most Recent
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Acta Pharm Sin B
Chrysin serves as a novel inhibitor of DGK α/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC). [Abstract]2021 Jan;11(1):143-155. PMID: 33532186 -
Sci Adv
Inhibition of fungal pathogenicity by targeting the H2S-synthesizing enzyme cystathionine β-synthase. [Abstract]2022 Dec 16;8(50):eadd5366. PMID: 36525499 -
J Enzyme Inhib Med Chem
Natural product sennoside B disrupts liquid-liquid phase separation of SARS-CoV-2 nucleocapsid protein by inhibiting its RNA-binding activity. [Abstract]2025 Dec;40(1):2501743. PMID: 40371698
Solvent & Solubility
DMSO : ≥ 100 mg/mL (218.16 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.54 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.54 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
Cell viability is examined by measuring the capability of cells to metabolize MTT to a purple formazan dye. Human liver cancer HepG2 cells are maintained in DMEM medium supplemented with 10% fetal bovine serum, 100 units/mL penicillin, and 50 units/mL streptomycin at 37°C in a humidified incubator with 5% CO2 atmosphere. Cells are seeded in 96-well tissue culture plates for 24 h and then incubated with the tested compounds at different concentrations for 72 h. After incubation, 25 μL MTT in 5 mg/mL PBS is added and incubated for 4 h. The medium is aspirated and replaced with 150 μL dimethyl sulfoxide (DMSO) to dissolve the formazan salt. The color intensity of the formazan solution, which reflects the cell growth condition, is measured at 570 nm using a microplate spectrophotometer.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Zhang LQ, et al. Accumulation of catechins and expression of catechin synthetic genes in Camellia sinensis at different developmental stages. Bot Stud. 2016 Dec;57(1):31. [Content Brief]
[2]. Zhou H, et al. C-geranylated flavanones from YingDe black tea and their antioxidant and α-glucosidase inhibition activities. Food Chem. 2017 Nov 15;235:227-233. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1816 mL | 10.9082 mL | 21.8164 mL | 54.5411 mL |
| 5 mM | 0.4363 mL | 2.1816 mL | 4.3633 mL | 10.9082 mL | |
| 10 mM | 0.2182 mL | 1.0908 mL | 2.1816 mL | 5.4541 mL | |
| 15 mM | 0.1454 mL | 0.7272 mL | 1.4544 mL | 3.6361 mL | |
| 20 mM | 0.1091 mL | 0.5454 mL | 1.0908 mL | 2.7271 mL | |
| 25 mM | 0.0873 mL | 0.4363 mL | 0.8727 mL | 2.1816 mL | |
| 30 mM | 0.0727 mL | 0.3636 mL | 0.7272 mL | 1.8180 mL | |
| 40 mM | 0.0545 mL | 0.2727 mL | 0.5454 mL | 1.3635 mL | |
| 50 mM | 0.0436 mL | 0.2182 mL | 0.4363 mL | 1.0908 mL | |
| 60 mM | 0.0364 mL | 0.1818 mL | 0.3636 mL | 0.9090 mL | |
| 80 mM | 0.0273 mL | 0.1364 mL | 0.2727 mL | 0.6818 mL | |
| 100 mM | 0.0218 mL | 0.1091 mL | 0.2182 mL | 0.5454 mL |
(-)-Gallocatechin gallate Related Classifications
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