1. MCE Kits
  2. Cell Biology
  3. Cell Culture

Cell Culture

FAQ

MCE Kits
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  • HY-K1014 PolyFast Transfection Reagent

    MCE PolyFast Transfection Reagent consists of cationic polymers and can introduce nucleic acids (DNA or RNA) into eukaryotic cells efficiently, including some hard-to-transfect cells.

    HY-K2014 PEI Transfection Reagent

    MCE PEI Transfection Reagent is designed based on 25 kDa PEI. It has high-efficiency, low-toxicity, strong-stability, and is suitable for many cell types, such as HEK-293、HEK-293T、CHO-K1、COS-1、COS-7、NIH/3T3、Sf9、HepG2 and HeLa et, even some hard-to-transfect cells. It can also be applied to large-scale recombinant protein expression and virus production.

    HY-K2015 Lentivirus Transfection Reagent

    MCE Lentivirus Transfection Reagent is a new type of transfection reagent based on cationic polymer, which is suitable for lentivirus packaging and transfection. It significantly improves the efficiency of packaging and transfection, resulting in the production of more recombinant lentivirus.

    HY-K2016 Protein Transfection Reagent

    MCE Protein Transfection Reagent is a cationic lipid mixture for complexation with proteins, peptides, antibodies and other biologically active molecules to allow their direct intracellular delivery.

    HY-K2017 siRNA/miRNA Transfection Reagent

    MCE siRNA/miRNA Transfection Reagent is a novel cationic polymer transfection reagent for siRNA and miRNA transfection.

Mycoplasma Clearance

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  • HY-K1059 BM-Cyclin

    MCE BM-Cyclin mainly contains Tiamulin fumarate and Minocycline hydrochloride, can effectively inhibit and remove mycoplasma contamination widespread in cell cultures. It is also effective against some common bacteria.

  • HY-K0014 Penicillin-Streptomycin-Neomycin (PSN) (100×), Sterile

    MCE Penicillin-Streptomycin-Neomycin (PSN) (100×), Sterile is used to prevent bacterial contamination of cell culture due to their effective combined action against gram-positive and gram-negative bacteria.

    HY-K1006 Penicillin-Streptomycin (100×), Sterile

    MCE MCE Penicillin-Streptomycin (100×), Sterile is used to prevent bacterial contamination of cell culture due to their effective combined action against gram-positive and gram-negative bacteria.

    HY-K1008 Gentamicin-Amphotericin B (500×), Sterile

    MCE Gentamicin-Amphotericin B (500×), sterile is used to prevent contamination of cell culture due to their effective combined action against gram-positive bacteria, gram-negative bacteria, fungi and yeasts.

    HY-K1010 Penicillin-Streptomycin-Glutamine (100×), Sterile

    MCE Penicillin-Streptomycin-Glutamine (100×), sterile is used to prevent bacterial contamination of cell culture due to their effective combined action against gram-positive and gram-negative bacteria.

    HY-K1044 Polymyxin B (1,000×), Sterile

    Polymixin B is a mixture of B1 and B2 polypeptides obtained from different strains of Bacillus polymyxa, with antibacterial activity against gram-negative bacteria. It can bind lipopolysaccharide (LPS) of the outer membrane of gram-negative bacteria and disrupts the cytoplasmic membrane by inducing large pores to allow nucleotide leakage in bacterial walls. This disrupts the permeability of the cytoplasmic membrane.

    HY-K1050 Gentamicin, Sterile

    MCE Gentamicin, Sterile acts by binding to the 30S subunit of the bacterial ribosome leading to inhibition of protein synthesis and death in susceptible bacteria, mainly for gram-negative and gram-positive bacteria.

    HY-K1051 Hygromycin B, Sterile

    MCE Hygromycin B, Sterile is an aminoglycosidic antibiotic purified from Streptomyces hygroscopicus. It acts by binding to the 70S subunit of the bacterial ribosome and inhibiting protein synthesis, leading to the death of bacteria, fungi and mammalian cells.

    HY-K1052 Amphotericin B, Sterile

    MCE Amphotericin B is a polyene antifungal antibiotic produced by Streptomyces nodosus. It can bind with sterols to form transmembrane channels, resulting in the leakage of intracellular substances, thus inhibiting fungal and yeast contamination.

    HY-K1054 Blasticidin S, Sterile

    Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation.

    HY-K1055 Kanamycin sulfate (100x), Sterile

    Kanamycin sulfate is an aminoglycoside antibiotic purified from Streptomyces kanamyceticus. It acts by binding to the 30S subunit of the bacterial ribosome and inhibiting protein synthesis in susceptible bacteria.

    HY-K1056 G418 Selective Antibiotic, Sterile

    MCE G418 Selective Antibiotic, Sterile is an aminoglycoside antibiotic produced by Micromonospora rhodorangea. It acts by binding to the 80S subunit of the bacterial ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells.

    HY-K1057 Puromycin, Sterile

    Puromycin is an aminonucleoside antibiotic produced by Streptomyces alboniger. It inhibits protein synthesis by disrupting peptide transfer on ribosomes, causing premature chain termination during translation. It can kill most gram-positive bacteria and various animal or insect cells.

    HY-K1058 Antibiotic-Antifungal (100 ×), Sterile

    MCE Antibiotic-Antifungal (100 ×), Sterile contains 10 KU/mL of penicillin, 10 mg/mL of streptomycin, and 25 μg/mL of Amphotericin B. The antibiotics penicillin and streptomycin prevent bacterial contamination, and Amphotericin B prevents fungal contamination.

CEPT cocktail

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  • HY-K1043 CEPT Cocktail

    MCE CEPT Cocktail is composed of CET small molecules (Chroman 1, Emricasan and Trans-ISRIB) and Polyamine Solution, and can effectively enhance cell survival and promote clonal growth of genetically stable hPSCs.

  • HY-K3001 DMEM (High Glucose, L-Glutamine, Pyruvate, Phenol Red, no HEPES)

    DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cell lines successfully cultured in DMEM include Hela, 293, Cos-7, and PC-12, as well as primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells.

    HY-K3002 DMEM/F-12 (1:1), L-Glutamine, Phenol Red, HEPES

    DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cell successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, etc.

    HY-K3003 DMEM (Low Glucose, L-Glutamine, Pyruvate, Phenol Red, no HEPES)

    DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cell lines successfully cultured in DMEM include Hela, 293, Cos-7, and PC-12, as well as primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, etc.

    HY-K3004 RPMI 1640 (L-Glutamine, Phenol Red, no HEPES)

    RPMI 1640 contains glutathione and high concentration of vitamins, also contains biotin, vitamin B12, 4-Aminobenzoic acid (PABA) not found in MEM and DMEM, as well as high concentration of inositol and choline chloride.

  • HY-K3005 PBS Buffer (1×)

    Phosphate-Buffered Saline (PBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells, diluting cells and preparing reagents.

    HY-K3006 PBS Buffer (10×)

    Phosphate-Buffered Saline (PBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells, diluting cells and preparing reagents.

    HY-K3008 DPBS Buffer (1×)

    Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells, diluting cells and preparing reagents.

  • HY-K0316 Serum Replacement

    MCE Serum Replacement is a fibrinogen-depleted and heparin-free Human Platelet Lysate. As a xeno-free and easy-to-use cell culture supplement, it can replace FBS (fetal bovine serum) to support the efficient and safe in vitro culture and expansion of various primary cells and cell lines.

    HY-K1046 L-Glutamine (100×), Sterile

    L-glutamine is an important amino acid supplement commonly added to mammalian cell culture media. L-glutamine serves as an auxiliary energy source, especially when cells are rapidly dividing. L-glutamine is also important in the production of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, other amino acids, and plays a role in protein synthesis and glucose production.

    HY-K3011 MEM Non-essential Amino Acid Solution (100×)

    MEM Non-essential Amino Acid Solution (100×) has been filtered and can be used directly. It adds nutrients and reduces the biosynthetic burden on cell in vitro.

    HY-K3012 N-2 Supplement (100×), Sterile

    N-2 Supplement promotes cell growth and survival. It has been associated with the growth and expression of neuroblastoma, and with post-mitotic neuronal survival and expression in primary cultures of both the peripheral nervous system (PNS) and central nervous system (CNS).

    HY-K3012A N-2 Serum-free Supplement (100×)

    MCE N-2 Serum-free Supplement (100×) can be used in conjunction with growth factors such as bFGF and EGF in serum-free neuronal cell culture mediums, DMEM and the DMEM/F12.

    HY-K3013 Bi-27 Supplement (50×), Sterile

    The most cited neuronal cell culture supplement, B-27 Supplement is an optimized serum-free supplement used to support the low- or high-density growth and short- or long-term viability of embryonic.

    HY-K3013A Bi-27 Serum-free Supplement (50×)

    MCE Bi-27 Serum-free Supplement (50×) can be used to support the low- or high-density growth and short- or long-term viability of embryonic.

    HY-K3014 Bi-27 Supplement (50×), Vitamin A-Free, Sterile

    Bi-27 Additive (50×), Vitamin A-Free is a customized variant of the standard Bi-27 formulation with vitamin A removed as a supplement for neuronal cell culture to support the low- or high-density growth and short- or long-term viability of embryonic.

    HY-K3014A Bi-27 Serum-free Supplement (50×), Vitamin A-Free

    MCE Bi-27 Serum-free Supplement (50×), Vitamin A-Free can be used to support the low- or high-density growth and short- or long-term viability of embryonic.

  • HY-K3007 0.25% Trypsin-EDTA (1x), phenol red

    MCE 0.25% Trypsin-EDTA (1x), phenol red consists of dried trypsin powder dissolved in EDTA. MCE Trypsin hydrolyzes intercellular proteins and disperses primary tissues or adherent cells into individual cells, and can be widely used for cell dissociation, cell culture passaging and primary tissue dissociation.

    HY-K3009 0.25% Trypsin-EDTA (1×), no phenol red

    MCE 0.25% Trypsin-EDTA (1×), Phenol Red-Free, is prepared by dissolving trypsin powder (a mixture of proteases from porcine pancreas, irradiated and sterilized) and EDTA in a calcium- and magnesium-free balanced salt solution. It is filter-sterilized and can be directly used for the dissociation of cultured cells and tissues.

    HY-K3010 Red Blood Cell Lysis Buffer

    MCE Red Blood Cell Lysis Buffer (10×) primarily contains ammonium chloride and is a ready-to-use solution designed for rapid and effective lysis and removal of anucleated red blood cells from human or mouse blood and tissue samples without affecting white blood cells, normal tissues, or tumor cells.