2. Apoptosis Stem Cell/Wnt Anti-infection
  3. Bacterial Apoptosis Wnt Fungal Parasite
  4. Prodigiosin

Prodigiosin  (Synonyms: Prodigiosine; NSC47147)

製品番号: HY-100711 純度: 99.84%
COA 取扱説明書 Technical Support

Prodigiosin (Prodigiosine) is a red pigment produced by bacteria as a bioactive secondary metabolite. Prodigiosin is a potent inhibitor of the Wnt/β-catenin pathway. Prodigiosin has antibacterial, antifungal, antiprotozoal, antimalarial, immunosuppressive, and anticancer properties.

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Prodigiosin

Prodigiosin 構造式

CAS 番号 : 82-89-3

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 550 在庫あり
Solution
10 mM * 1 mL in DMSO USD 550 在庫あり
Solid
100 μg $50 在庫あり
250 μg $90 在庫あり
500 μg $150 在庫あり
1 mg $240 在庫あり
5 mg $500 在庫あり
10 mg $750 在庫あり
50 mg   お問い合わせ  
100 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 3 publication(s) in Google Scholar

Other Forms of Prodigiosin:

Prodigiosin 関連抗体

Top Publications Citing Use of Products

Wnt アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
Wnt1 Wnt3 Wnt5 Wnt7 Wnt11

Parasite アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

  • 生物活性

  • 純度とドキュメンテーション

  • 参考文献

  • カスタマーレビュー

製品説明

Prodigiosin (Prodigiosine) is a red pigment produced by bacteria as a bioactive secondary metabolite. Prodigiosin is a potent inhibitor of the Wnt/β-catenin pathway. Prodigiosin has antibacterial, antifungal, antiprotozoal, antimalarial, immunosuppressive, and anticancer properties[1][2].

IC50 & Target

Plasmodium

 

Cellular Effect
Cell Line Type Value Description References
A549 IC50
0.077 μM
Compound: 1
Antiproliferative activity against A549 cell line by MTS assay
Antiproliferative activity against A549 cell line by MTS assay
[PMID: 16289814]
DLD-1 IC50
0.079 μM
Compound: 1
Antiproliferative activity against DLD1 cell line by MTS assay
Antiproliferative activity against DLD1 cell line by MTS assay
[PMID: 16289814]
HepG2 IC50
0.04 μM
Compound: 27
Anticancer activity against human HepG2 cells by MTT assay
Anticancer activity against human HepG2 cells by MTT assay
[PMID: 34128674]
HT-29 IC50
0.173 μM
Compound: 1
Antiproliferative activity against HT29 cell line by MTS assay
Antiproliferative activity against HT29 cell line by MTS assay
[PMID: 16289814]
MDA-MB-231 IC50
0.213 μM
Compound: 1
Antiproliferative activity against MDA-MB-231 cell line by MTS assay
Antiproliferative activity against MDA-MB-231 cell line by MTS assay
[PMID: 16289814]
NCI-H460 IC50
0.032 μM
Compound: 1
Antiproliferative activity against NCI-H460 cell line by MTS assay
Antiproliferative activity against NCI-H460 cell line by MTS assay
[PMID: 16289814]
SK-OV-3 IC50
1.3 μM
Compound: Prodigiosin
Cytotoxicity against human SKOV3 cells after 48 hrs by SRB assay
Cytotoxicity against human SKOV3 cells after 48 hrs by SRB assay
[PMID: 18922034]
体外実験

Prodigiosin (25-500 nM; 24 hours) treatment reduces the viability of breast cancer cells, with IC50 values at 48 h of 62.52 nM in MDA-MB-231 cells and 261.2 nM in MDA-MB-468 cells[1].
Prodigiosin (25-500 nM; 24 hours) treatment significantly reduces the levels of phosphorylated LRP6 and DVL2, active β-catenin, and total β-catenin. Prodigiosin noticeably inhibits the phosphorylation of GSK3β at Ser9 in HEK293T cells, which is indicative of an increase in GSK3β activity[1].
Prodigiosin can inhibit proliferation and induce apoptosis in breast cancer cells[1].
Prodigiosin (25-500 nM; 24 hours) treatment dose-dependently blocks Wnt signaling activated by Wnt1, Wnt3, Wnt1/LRP6, Wnt3/LRP6, and Dishevelled 2 (DVL2) in transfected HEK293T cells. Prodigiosin treatment inhibits Wnt3A-CM-induced transcription in a dose-dependent manner. Prodigiosin inhibits transcription of the SuperTopFlash reporter activated by either Wnt transfection or Wnt3A treatment[1].
When applied to cultures of chytrid fungi Batrachochytrium dendrobatidis and B. salamandrivorans, Prodigiosin causes significant growth inhibition, with MIC values of 10 μM and 50 μM, respectively[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Prodigiosin 関連抗体

Cell Proliferation Assay[1]

Cell Line: MDA-MB-231 and MDA-MB-468 cells
Concentration: 10 nM, 25 nM, 50 nM, 100 nM, 250 nM, 500 nM, 1000 nM, 2500 nM, 5000 nM
Incubation Time: 24 hours, 48 hours
Result: Reduced the viability of breast cancer cells, with IC50 values at 48 h of 62.52 nM in MDA-MB-231 cells and 261.2 nM in MDA-MB-468 cells.

Western Blot Analysis[1]

Cell Line: HEK293T cells
Concentration: 50 nM, 100 nM, 250 nM, 500 nM
Incubation Time: 24 hours
Result: Significantly reduced the levels of phosphorylated LRP6 and DVL2, active β-catenin, and total β-catenin.
体内実験

Prodigiosin (5 mg/kg; intraperitoneal injection; twice weekly; for 3 weeks) treatment significantly inhibits tumor growth. Prodigiosin treatment decreases tumor cell density and expression of the proliferation marker Ki-67[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female BALB/c nude mice injected with MDA-MB-231 cells[1]
Dosage: 5 mg/kg
Administration: Intraperitoneal injection; twice weekly; for 3 weeks
Result: Significantly inhibited tumor growth in mice.
分子量

323.43

分子式

C20H25N3O
CAS 番号
Appearance

Solid

Color

Brown to red

SMILES

CCCCCC1=C/C(N=C1C)=C\C2=C(OC)C=C(C3=CC=CN3)N2
Structure Classification
Initial Source

Serratia marcescens
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : ≥ 25 mg/mL (77.30 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0919 mL 15.4593 mL 30.9186 mL
5 mM 0.6184 mL 3.0919 mL 6.1837 mL
10 mM 0.3092 mL 1.5459 mL 3.0919 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  2% DMSO    40% PEG300    5% Tween-80    53% Saline

    Solubility: ≥ 0.5 mg/mL (1.55 mM); Clear solution

  • Protocol 2

    Add each solvent one by one:  2% DMSO    98% (20% SBE-β-CD in Saline)

    Solubility: 0.5 mg/mL (1.55 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.84%

COA (248 KB) HNMR (195 KB) RP-HPLC (259 KB) MS (202 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0919 mL 15.4593 mL 30.9186 mL 77.2965 mL
5 mM 0.6184 mL 3.0919 mL 6.1837 mL 15.4593 mL
10 mM 0.3092 mL 1.5459 mL 3.0919 mL 7.7296 mL
15 mM 0.2061 mL 1.0306 mL 2.0612 mL 5.1531 mL
20 mM 0.1546 mL 0.7730 mL 1.5459 mL 3.8648 mL
25 mM 0.1237 mL 0.6184 mL 1.2367 mL 3.0919 mL
30 mM 0.1031 mL 0.5153 mL 1.0306 mL 2.5765 mL
40 mM 0.0773 mL 0.3865 mL 0.7730 mL 1.9324 mL
50 mM 0.0618 mL 0.3092 mL 0.6184 mL 1.5459 mL
60 mM 0.0515 mL 0.2577 mL 0.5153 mL 1.2883 mL
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Prodigiosin Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Prodigiosin82-89-3Prodigiosine NSC47147NSC47147NSC 47147NSC-47147BacterialApoptosisWntFungalParasiteAntibacterialantifungalantiprotozoalantimalarialimmunosuppressiveanticancerpigmentβ-cateninInhibitorinhibitorinhibit

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