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Results for "

fixed cells

" in MedChemExpress (MCE) Product Catalog:

35

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28

Fluorescent Dyes

2

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2

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3

Click Chemistry

Cat. No. Product Name Target Research Areas Chemical Structure
  • HY-W090090
    BODIPY 493/503
    100+ Cited Publications

    Pyrromethene 546

    Fluorescent Dye Others
    BODIPY493/503 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells . Maximum excitation/emission wavelength: 493/503 nm .
    BODIPY 493/503
  • HY-D0814
    DAPI dihydrochloride
    Maximum Cited Publications
    146 Publications Verification

    4',6-Diamidino-2-phenylindole dihydrochloride

    DNA Stain Sodium Channel Neurological Disease
    DAPI (4',6-Diamidino-2-phenylindole) dihydrochloride is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells . DAPI (Compound 3) is an acid-sensing ion channel 3 (ASIC3) inhibitor. DAPI binds to ASIC3 and blocks the channel function. DAPI can be used in the study of chronic pain treatment (Ex/Em = 356/451 nm).
    DAPI dihydrochloride
  • HY-13735
    Quinacrine
    5+ Cited Publications

    Acriquine

    Parasite Sodium Channel DNA Stain Apoptosis Aldehyde Oxidase (AO) Infection Neurological Disease Cancer
    Quinacrine (Acriquine) is an antimalarial and anti-cancer agent. Quinacrine also inhibits human aldehyde oxidase (IC50: 3.3 μM). Quinacrine has affinity for nucleic acids, and stains DNA and RNA in fixed cells (Ex/Em: 436/525 nm) .
    Quinacrine
  • HY-D1237
    BODIPY 505/515
    2 Publications Verification

    Fluorescent Dye Metabolic Disease
    BODIPY505/515 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells . Maximum excitation/emission wavelength: 505/515 nm .
    BODIPY 505/515
  • HY-D1614
    BODIPY 493/503 methyl bromide
    15+ Cited Publications

    Fluorescent Dye Others
    BODIPY493/503 methyl bromide is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells . Maximum excitation/emission wavelength: 493/503 nm .
    BODIPY 493/503 methyl bromide
  • HY-D1106

    Fluorescent Dye Cancer
    BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions . Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells .
    Py-BODIPY-NHS ester
  • HY-138226
    BODIPY 558/568 C12
    4 Publications Verification

    Fluorescent Dye Others
    BODIPY 558/568 C12 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells . Maximum excitation/emission wavelength: 558/568 nm .
    BODIPY 558/568 C12
  • HY-15558
    Hoechst 33258
    20+ Cited Publications

    bisBenzimide H 33258; H 33258

    Fluorescent Dye DNA Stain DNA Alkylator/Crosslinker Others
    Hoechst 33258 is a blue to blue-green fluorescent live cell dye that can label DNA. Hoechst 33258 can specifically bind to the minor groove of DNA (and tends to bind to A/T-rich DNA), resulting in a significant increase in fluorescence intensity. Hoechst 33258 can cross the cell membrane and cause changes in DNA structure, such as G2/M phase arrest. Hoechst 33258 can bind to live or fixed cells, and the fluorescence intensity increases with increasing solution pH. As a DNA-specific probe, Hoechst 33258 can be used to detect DNA content, analyze cell cycle, etc. The excitation wavelength of Hoechst 33258 is 350-365 nm, and the emission wavelength is 460-490 nm .
    Hoechst 33258
  • HY-DY1004

    Fluorescent Dye Others
    BODIPY 493/503 (solution) is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells . Maximum excitation/emission wavelength: 493/503 nm.
    Solvent and concentration: DMSO: 5 mM.
    BODIPY 493/503 (solution)
  • HY-D1738
    DAPI dilactate
    110+ Cited Publications

    4',6-Diamidino-2-phenylindole dilactate

    Fluorescent Dye Others
    DAPI (4',6-Diamidino-2-phenylindole) dilactate is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. DAPI cannot penetrate intact cell membranes and is commonly used for staining both live and fixed cells . DAPI (Compound 3) is an acid-sensing ion channel 3 (ASIC3) inhibitor. DAPI binds to ASIC3 and blocks the channel function. DAPI can be used in the study of chronic pain treatment (Ex/Em = 356/451 nm) .
    DAPI dilactate
  • HY-D1429

    Fluorescent Dye Others
    ER-Tracker dye is a derivative of BODIPY series dyes coupled with Glibenclamide (HY-15206), highly selective binding to the endoplasmic reticulum, non-toxic to cells at low concentrations, this type of dye is an environmentally sensitive probe, and formaldehyde treatment can still retain part of the fluorescence, with high fluorescence life, good extinction coefficient and other characteristics. Glibenclamide is an atp-dependent K + channel blocker (Kir6, KATP) and CFTR Cl-channel blocker that binds in the endoplasmic reticulum. ER-Tracker is not suitable for staining fixed cells. Staining followed by fixation is possible, but cells fixed with aldehydes will only retain partial fluorescence (Ex/Em = 374/ 430-640 nm) .
    ER-Tracker Blue-White DPX
  • HY-W356116

    Nbd-ceramide

    Fluorescent Dye Others
    C6 NBD Ceramide is a Golgi apparatus fluorescent probe with cell membrane permeability. C6 NBD Ceramide can be used for fast and convenient green fluorescent labeling of Golgi in living and fixed cells, and can be used to observe changes in Golgi morphology in living cells (Ex=466 nm, Em=536 nm). C6-NBD-ceramide is metabolized to fluorescent sphingomyelin and glucosylceramide, can be used for the study of sphingolipid transport and metabolic mechanism .
    C6 NBD Ceramide
  • HY-D1570

    Fluorescent Dye Others
    BODIPYFL C12 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells . Maximum excitation/emission wavelength: 480/508 nm .
    BODIPY FL C12
  • HY-D1617
    BODIPY 500/510 C1, C12
    2 Publications Verification

    Fluorescent Dye Others
    BODIPY 500/510 C1, C12 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells . Maximum excitation/emission wavelength: 500/510 nm . Protect from light, stored at -20°C.
    BODIPY 500/510 C1, C12
  • HY-P10932A

    Fluorescent Dye Neurological Disease
    pGk13a TFA is an azide (azide group can be combined with fluorophore)-containing amphiphilic membrane labeling probe. pGk13a TFA enables high-resolution imaging of cell membranes in the ultrastructural membrane expansion microscopy (umExM) technique, facilitating the observation of membrane-associated structures and proteins. pGk13a TFA can be used for neuronal structural studies .
    pGk13a TFA
  • HY-D2868
    DAPI
    130+ Cited Publications

    4',6-Diamidino-2-phenylindole

    DNA Stain Sodium Channel Neurological Disease
    DAPI (4',6-Diamidino-2-phenylindole) is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells . DAPI (Compound 3) is an acid-sensing ion channel 3 (ASIC3) inhibitor. DAPI binds to ASIC3 and blocks the channel function. DAPI can be used in the study of chronic pain treatment (Ex/Em = 356/451 nm).
    DAPI
  • HY-DY1074

    Fluorescent Dye Others
    ER-Tracker dye is a derivative of BODIPY series dyes coupled with Glibenclamide (HY-15206) , highly selective binding to the endoplasmic reticulum, non-toxic to cells at low concentrations, this type of dye is an environmentally sensitive probe, and formaldehyde treatment can still retain part of the fluorescence, with high fluorescence life, good extinction coefficient and other characteristics. Glibenclamide is an atp-dependent K + channel blocker (Kir6, KATP) and CFTR Cl-channel blocker that binds in the endoplasmic reticulum. ER-Tracker is not suitable for staining fixed cells. Staining followed by fixation is possible, but cells fixed with aldehydes will only retain partial fluorescence .
    Solvent and concentration: DMSO: 1 mM
    ER-Tracker Blue-White DPX (solution)
  • HY-D1422

    BDNCA-346

    Fluorescent Dye Others
    ER Tracker Yellow is an endoplasmic reticulum-targeted temperature-visible fluorescent probe. ER Tracker Yellow has high temperature sensitivity (3.9%/°C) and is not affected by pH and Ca 2+ interference. ER Tracker Yellow can be used for real-time monitoring of local temperature changes in the ER area. ER Tracker Yellow maintains its function in fixed cells. ER Tracker Yellow is suitable for various cell lines and primary cells, such as HeLa cells, MEF cells, and other cells .
    ER Tracker Yellow
  • HY-D1261
    Naph-EA-mal
    1 Publications Verification

    Thiol-green 1

    Fluorescent Dye Others
    Naph-EA-mal (Thiol-green 1) is a rapid detect and ultrafast turn-on thiol fluorescence probe for protein labeling and bioimaging. Naph-EA-mal (Thiol-green 1) can be used to detect thiols in living cells, label the protein thiols, quantify the concentration of total thiols in cell lysate, and determine the reversible protein thiols oxidation in fixed cells . Ex: 488 nM; Em: 540 nM.
    Naph-EA-mal
  • HY-156404
    PM-1
    2 Publications Verification

    Fluorescent Dye Others
    PM-1, a derivative of Thioflavin-T (ThT; HY-D0218), is a small but highly specific plasma membrane (PM) fluorescent dye for specific and long-time membrane imaging of living and fixed cells. PM-1 is embedded directly into the cell membrane and exhibits a very long retention time on the plasma membrane with a half-life of approximately 15 h. PM-1 can be used in combination with protein labeling probes to study ectodomain shedding and endocytosis processes of cell surface proteins .
    PM-1
  • HY-DY1061

    Fluorescent Dye Others
    BODIPY 558/568 C12 (solution) is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells . Maximum excitation/emission wavelength: 558/568 nm .
    Solvent and concentration: DMSO: 2 mM
    BODIPY 558/568 C12 (solution)
  • HY-D1991

    Fluorescent Dye Others
    ATTO 647 is a carborhodamine fluorophore and imaging tracer with photostable properties. ATTO 647 serves as a fluorescent probe to investigate cell membrane structure and diffusion characteristics. When conjugated with wheat germ agglutinin, ATTO 647 specifically binds to N-acetyl-β-D-glucosamine and sialic acid residues on membrane glycoproteins, enabling single-molecule tracing of glycoprotein diffusion. ATTO 647 exhibits highly stable fluorescence properties with significantly reduced blinking in mounting media such as ROXS (AA/MV) and ROXS (TX/TQ), whereas its brightness properties vary in Ibidi-MM and Vectashield. ATTO 647 can also be used to label histone H2B-GFP in fixed cells for confocal microscopy photobleaching experiments .
    ATTO 647
  • HY-151759

    Fluorescent Dye Cancer
    Sulfo-Cy5-TCO is a water-soluble sulfonated Cy5 fluorescent reagent containing trans-cyclooctene (TCO). Sulfo-Cy5-TCO undergoes an inverse electron demand Diels‑Alder reaction with tetrazine-labeled fixed breast cancer cells to achieve cell labeling. Sulfo-Cy5-TCO is used for fluorescent labeling and imaging of biological samples such as proteins and cells .
    Sulfo-Cy5-TCO
  • HY-P10932

    Fluorescent Dye Neurological Disease
    pGk13a is an amphipathic membrane-labeling probe containing an azide group, which can bind to fluorophores. pGk13a enables high-resolution imaging of cell membranes in the ultrastructure expansion microscopy (umExM) technique, facilitating the observation of membrane-associated structures and proteins. pGk13a is applicable to neuronal structure research .
    pGk13a
  • HY-D2994

    SNAP-MaP555

    Fluorescent Dye Others
    SPY555-BG (SNAP-MaP555) is the benzylguanine derivative of SPY555 fluorophore. SPY555-BG emits light in the orange part of the UV-ViS spectrum. SPY555-BG is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SPY555-BG can be imaged with a standard Cy3 filterset. SPY555-BG can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue .
    SPY555-BG
  • HY-W615095

    PROTAC Linkers Others
    Biotin-PEG2-alkyne is a click chemistry PEG linker based on copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC), as well as a biotinylation reagent. In solution-phase and click labeling systems, Biotin-PEG2-alkyne efficiently conjugates biotin groups to azide-functionalized streptavidin amplicons. Biotin-PEG2-alkyne is also widely applicable in fixed cells and mouse intestinal tissue sections to achieve specific biotinylation of various azide-labeled biomolecules (such as proteins, lipids, DNA, and Afatinib (HY-10261)), thereby effectively promoting signal amplification and precise detection of targets .
    Biotin-PEG2-alkyne
  • HY-13735R

    Acriquine (Standard)

    Parasite Sodium Channel DNA Stain Apoptosis Reference Standards Infection Neurological Disease Cancer
    Quinacrine (Standard) is the analytical standard of Quinacrine. This product is intended for research and analytical applications. Quinacrine (Acriquine) is an antimalarial and anti-cancer agent. Quinacrine also inhibits human aldehyde oxidase (IC50: 3.3 μM). Quinacrine has affinity for nucleic acids, and stains DNA and RNA in fixed cells (Ex/Em: 436/525 nm) .
    Quinacrine (Standard)
  • HY-D1396
    Br-DAPI
    3 Publications Verification

    Fluorescent Dye DNA Stain Others
    Br-DAPI is a marker dye in DAPI series. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells . Storage: Keep away from light.
    Br-DAPI
  • HY-D1745

    fac-[Re(CO)3(phen)L]

    Fluorescent Dye Others
    Liptracker-green (fac-[Re(CO)3(phen)L]) is a cell-permeable fluorogenic probe that localizes to polar lipids, and can be used to label lipid droplets and other high lipid-content compartments in live and fixed cells using fluorescent microscopy.
    Liptracker-Green
  • HY-D2758

    Fluorescent Dye Others
    TAMRA DBCO, 5-isomer is a derivative of TAMRA fluorescent isomer with a DBCO functional group. TAMRA DBCO, 5-isomer undergoes strain-promoted azide-alkyne cycloaddition (SPAAC) under copper-free catalytic conditions. TAMRA DBCO, 5-isomer serves as a FRET acceptor for FAM fluorophores. TAMRA DBCO, 5-isomer can label proteins, peptides, nucleic acids and all azide-modified biomolecules, and is suitable for fluorescence imaging and flow cytometry to quantify intracellular SPAAC reactions .
    TAMRA DBCO, 5-isomer
  • HY-D3446

    Fluorescent Dye Others
    PA-SiR-Halo is a photoactivatable fluorophore targeting HaloTag. When bound to HaloTag, PA-SiR-Halo stabilizes its fluorescent form under physiological pH. After conjugation and photoactivation, it exhibits enhanced resistance to nucleophiles, and enables live-cell confocal imaging, fixed-cell single-molecule localization microscopy imaging, live-cell single-particle tracking, as well as live-cell single-molecule localization microscopy imaging of dynamic mitochondrial structures without the need for washing .
    PA-SiR-Halo
  • HY-DY1090

    Fluorescent Dye Others
    BODIPY 493/503 methyl bromide (solution) is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells . Maximum excitation/emission wavelength: 493/503 nm .
    Solvent and concentration: DMSO: 5 mM
    BODIPY 493/503 methyl bromide (solution)
  • HY-W800699

    Fluorescent Dye Others
    BP Fluor 430 DBCO reacts with azides via a copper-free click chemistry reaction to form a stable triazole and does not require a Cu-catalyst or elevated temperatures. In applications where the presence of copper is a concern, BP Fluor 430 DBCO is an ideal alternative to copper requiring fluorescent alkynes. BP Fluor 430 is a bright, photostable, green-fluorescent probe optimally excited near its absorption maximum at 432 nm. Its emission peak at 539 nm is pH independent over a wide pH range. BP Fluor DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.
    BP Fluor 430 DBCO
  • HY-D1737

    Bacterial Infection
    RADA is a fluorescent D-amino acid (FDAA) with high photostability and thermostability, which emits yellow-to-orange fluorescence. RADA shows low outer membrane permeability in wild-type Gram-negative Escherichia coli, but it targets penicillin-binding proteins and L,D-transpeptidases, mimics the interaction between acyl acceptors and enzyme intermediates, and integrates into peptidoglycan during biosynthesis. As a peptidoglycan labeling reagent, RADA metabolically integrates into the nascent peptidoglycan of live bacterial cells, labels the peptidoglycan at the poles and lateral walls of mycobacteria, and enables visualization of peptidoglycan synthesis and remodeling processes. RADA serves as a non-specific stain for fixed cells, is non-toxic to bacterial cells, and its red-shifted excitation/emission spectra reduce phototoxicity. RADA also supports virtual pulse-chase labeling experiments and stochastic optical reconstruction microscopy for sub-diffraction-limited imaging of bacterial cell walls .
    RADA
  • HY-DY1081

    4',6-Diamidino-2-phenylindole dihydrochloride (solution)

    Sodium Channel DNA Stain Neurological Disease
    DAPI (4',6-Diamidino-2-phenylindole) dihydrochloride (solution) is a DAPI dye. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells . DAPI (Compound 3) is an acid-sensing ion channel 3 (ASIC3) inhibitor. DAPI binds to ASIC3 and blocks the channel function. DAPI can be used in the study of chronic pain treatment (Ex/Em = 356/451 nm).
    Solvent and concentration: ddH2O: 5 mg/mL
    DAPI dihydrochloride (solution)

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