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C6 NBD Ceramide 

Cat. No.: HY-W356116
Handling Instructions

C6 NBD Ceramide is a Golgi apparatus fluorescent probe with cell membrane permeability. C6 NBD Ceramide can be used for fast and convenient green fluorescent labeling of Golgi in living and fixed cells, and can be used to observe changes in Golgi morphology in living cells (Ex=466 nm, Em=536 nm). C6-NBD-ceramide is metabolized to fluorescent sphingomyelin and glucosylceramide, can be used for the study of sphingolipid transport and metabolic mechanism.

For research use only. We do not sell to patients.

C6 NBD Ceramide Chemical Structure

C6 NBD Ceramide Chemical Structure

CAS No. : 94885-02-6

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Description

C6 NBD Ceramide is a Golgi apparatus fluorescent probe with cell membrane permeability. C6 NBD Ceramide can be used for fast and convenient green fluorescent labeling of Golgi in living and fixed cells, and can be used to observe changes in Golgi morphology in living cells (Ex=466 nm, Em=536 nm). C6-NBD-ceramide is metabolized to fluorescent sphingomyelin and glucosylceramide, can be used for the study of sphingolipid transport and metabolic mechanism[1][2][3].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Golgi apparatus[3]:
1. Rinse cells (on glass cover slips) by HMEM, then fix cells in glutaraldehyde or paraformaldehyde for 5-10 min (25℃).
2. Wash fixed cells in a balanced salt solution (such as HCMF).
3. Transfer culture dishes containing the glass cover slips to an ice-water bath and incubate with freshly prepared NaBH4 for 3×5 min (for glutaraldehyde-fixed cells).
4. Rinse cells several times (20-30 min) in cold HCMF, warm to 25℃, then incubate with C6 NBD-ceramide/BSA for 60 min.
5. Wash fixed cells by HCMF, and incubate with 10% fetal calf serum or 2 mg/mL BSA (30-90 min; 25℃) to back-exchange excess C6 NBD-ceramide from the preparation and enhance the staining of the Golgi apparatus.
6. The glass cover slips mounted on depression slides and observe by fluorescence microscope (Ex=466 nm, Em=536 nm).

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

575.74

Formula

C30H49N5O6

CAS No.
SMILES

CCCCCCCCCCCCC/C=C/[[email protected]@H](O)[[email protected]@H](NC(CCCCCNC1=CC=C([N+]([O-])=O)C2=NON=C12)=O)CO

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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C6 NBD Ceramide
Cat. No.:
HY-W356116
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