2. Anti-infection
  3. Bacterial
  4. RADA

RADA is a fluorescent D-amino acid (FDAA) with high photostability and thermostability, which emits yellow-to-orange fluorescence. RADA shows low outer membrane permeability in wild-type Gram-negative Escherichia coli, but it targets penicillin-binding proteins and L,D-transpeptidases, mimics the interaction between acyl acceptors and enzyme intermediates, and integrates into peptidoglycan during biosynthesis. As a peptidoglycan labeling reagent, RADA metabolically integrates into the nascent peptidoglycan of live bacterial cells, labels the peptidoglycan at the poles and lateral walls of mycobacteria, and enables visualization of peptidoglycan synthesis and remodeling processes. RADA serves as a non-specific stain for fixed cells, is non-toxic to bacterial cells, and its red-shifted excitation/emission spectra reduce phototoxicity. RADA also supports virtual pulse-chase labeling experiments and stochastic optical reconstruction microscopy for sub-diffraction-limited imaging of bacterial cell walls.

For research use only. We do not sell to patients.

RADA

RADA Chemical Structure

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Based on 1 publication(s) in Google Scholar

RADA Related Antibodies

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Description

RADA is a fluorescent D-amino acid (FDAA) with high photostability and thermostability, which emits yellow-to-orange fluorescence. RADA shows low outer membrane permeability in wild-type Gram-negative Escherichia coli, but it targets penicillin-binding proteins and L,D-transpeptidases, mimics the interaction between acyl acceptors and enzyme intermediates, and integrates into peptidoglycan during biosynthesis. As a peptidoglycan labeling reagent, RADA metabolically integrates into the nascent peptidoglycan of live bacterial cells, labels the peptidoglycan at the poles and lateral walls of mycobacteria, and enables visualization of peptidoglycan synthesis and remodeling processes. RADA serves as a non-specific stain for fixed cells, is non-toxic to bacterial cells, and its red-shifted excitation/emission spectra reduce phototoxicity. RADA also supports virtual pulse-chase labeling experiments and stochastic optical reconstruction microscopy for sub-diffraction-limited imaging of bacterial cell walls[1].

In Vitro

RADA (25 μM; 15 min) labels both the poles and sidewall of Mycobacterium smegmatis cells, marking peptidoglycan remodeling that exhibits asymmetric polar gradients coincident with mycomembrane metabolism[1].
RADA (25 μM; 15 min) labeling in Mycobacterium smegmatis is significantly inhibited by imipenem (HY-B1369A) and D-cycloserine (HY-B0030), with greater sensitivity to imipenem than other monopeptide D-amino acid probes[1].
RADA (25 μM; 15 min chase) labeling of Mycobacterium smegmatis shows fluorescence dilution confined to the poles during outgrowth, confirming polar-restricted cell envelope expansion with faster growth at the RADA-bright pole[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RADA Related Antibodies
Molecular Weight

516.55

Formula

C28H28N4O6
SMILES

C/[N+](C)=C(C=C1)/C=C(C1=C2C3=C(C([O-])=O)C=C(C(NC[C@@H](N)C(O)=O)=O)C=C3)OC4=C2C=CC(N(C)C)=C4.C/[N+](C)=C(C=C5)/C=C(C5=C6C7=C(C([O-])=O)C=CC(C(NC[C@@H](N)C(O)=O)=O)=C7)OC8=C6C=CC(N(C)C)=C8
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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RADA Related Classifications

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The dilution calculator equation

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This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RADABacterialEscherichia coliLdtAMycobacterium smegmatisLdtBL,D-transpeptidasesMycobacterium tuberculosisBacillus subtilispenicillin-binding proteinsLdtEpeptidoglycanInhibitorinhibitorinhibit

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