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  3. BODIPY 558/568 C12

BODIPY 558/568 C12 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the polar lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells. Maximum excitation/emission wavelength: 558/568 nm.

For research use only. We do not sell to patients.

BODIPY 558/568 C12 Chemical Structure

BODIPY 558/568 C12 Chemical Structure

CAS No. : 158757-84-7

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1 mg USD 220 In-stock
5 mg USD 550 In-stock
10 mg USD 850 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

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Description

BODIPY 558/568 C12 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the polar lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells[1]. Maximum excitation/emission wavelength: 558/568 nm[1].

In Vitro

General Protocol
1. Preparation of BODIPY558/568C12 working solution
1.1 Preparation of the stock solution
Dissolve 1 mg BODIPY558/568C12 in 382 μL DMSO to obtain 10 mM of stock solution.
Note: It is recommended to store the stock solution at -20°C or -80°C, keep away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of BODIPY558/568C12 working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of BODIPY558/568C12 working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a. Centrifuge at 1000 g at 4°C for 3-5 min and then discard the supernatant. Wash twice with PBS, 5 min each time.The cell density is 1×106/mL.
b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 min.
c.Centrifuge at 400 g at 4°C for 3-4 min and then discard the supernatant.
d. Wash twice with PBS, 5 min each time.
e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 min.
d. Wash twice with medium, 5 min each time. Observation by fluorescence microscopy or flow cytometry.

Storage
-20°C, 1 year; Protect from light.
Precautions
1. Please adjust the concentration of BODIPY558/568C12 working solution according to the actual situation.
2. Experiments suggest a positive control, incubate the control group cells with 30 μM oleic acid for 8 h and then perform subsequent experiments.
3. This product is only for R&D use, not for drug, household, or others.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

472.40

Formula

C25H31BF2N2O2S

CAS No.
Appearance

Solid

Color

Brown to breen

SMILES

[F-][B+3]([N]1=C(C2=CC=CS2)C=CC1=CC3=CC=C4CCCCCCCCCCCC(O)=O)([N-]34)[F-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (264.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1169 mL 10.5843 mL 21.1685 mL
5 mM 0.4234 mL 2.1169 mL 4.2337 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.13%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1169 mL 10.5843 mL 21.1685 mL 52.9213 mL
5 mM 0.4234 mL 2.1169 mL 4.2337 mL 10.5843 mL
10 mM 0.2117 mL 1.0584 mL 2.1169 mL 5.2921 mL
15 mM 0.1411 mL 0.7056 mL 1.4112 mL 3.5281 mL
20 mM 0.1058 mL 0.5292 mL 1.0584 mL 2.6461 mL
25 mM 0.0847 mL 0.4234 mL 0.8467 mL 2.1169 mL
30 mM 0.0706 mL 0.3528 mL 0.7056 mL 1.7640 mL
40 mM 0.0529 mL 0.2646 mL 0.5292 mL 1.3230 mL
50 mM 0.0423 mL 0.2117 mL 0.4234 mL 1.0584 mL
60 mM 0.0353 mL 0.1764 mL 0.3528 mL 0.8820 mL
80 mM 0.0265 mL 0.1323 mL 0.2646 mL 0.6615 mL
100 mM 0.0212 mL 0.1058 mL 0.2117 mL 0.5292 mL
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Product Name:
BODIPY 558/568 C12
Cat. No.:
HY-138226
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