BODIPY 558/568 C12
Based on 5 publication(s) in Google Scholar
BODIPY 558/568 C12 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells. Maximum excitation/emission wavelength: 558/568 nm.
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- Pureté: 98.42%
- CAS No.: 158757-84-7
- Formule: C25H31BF2N2O2S
- Masse moléculaire:472.40
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Stockage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) BODIPY 558/568 C12
More
Customer Validation & Images
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Flow Cytometry
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Flow Cytometry
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Cell Imaging/Staining
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Flow Cytometry
Activité biologique
Guide (The following is our recommended experimental protocol. This protocol serves as a reference guide only. Specific operations should be adjusted according to your actual needs.)
1. Preparation of Hoechst solution
1. Preparation of BODIPY558/568C12 solution
1.1 Prepare the storage solution
Prepare a 10 mM solution.
1.2 Preparation of the working solution
Dilute the storage solution with preheated serum-free cell culture medium or PBS to prepare a 1-10 μM BODIPY558/568C12 working solution.
Note: Please adjust the concentration of BODIPY558/568C12 working solution according to the actual situation, and prepare it as needed.
2. Cell staining (suspended cells)
2.1 Centrifuge to collect cells, add PBS for two washes, each for 5 minutes. Cell density is 1×106/mL
2.2 Add 1 mL of BODIPY558/568C12 working solution, incubate at room temperature for 5-30 minutes.
2.3 Centrifuge at 400 g for 3-4 minutes, discard the supernatant.
2.4 Add PBS for two washes, each for 5 minutes.
2.5 Resuspend the cells in 1 mL of serum-free medium or PBS, then observe using a fluorescence microscope or flow cytometer.
3. Cell staining (adherent cells)
3.1 Place adherent cells on a sterile cover glass.
3.2 Remove the cover glass from the culture medium, remove excess medium.
3.3 Add 100 μL of the dye working solution, gently shake to fully cover the cells, incubate for 5-30 minutes.
3.4 Remove the dye working solution, wash twice with culture medium, each for 5 minutes, and observe using a fluorescence microscope or flow cytometer.
Notes
1. Please adjust the concentration of BODIPY558/568C12 working solution and incubation time according to the actual situation.
2. It is recommended to conduct a positive control experiment. Place the control group cells in 30 μM oleic acid for 8 hours before proceeding with the subsequent experiments.
3. This product is only for professional scientific research use and must not be used for clinical diagnosis or treatment, nor for food or medicine.
4. To ensure your safety and health, please wear laboratory clothing and use disposable gloves during the operation.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 158757-84-7
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Appearance Solid
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Masse moléculaire 472.40
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Formule C25H31BF2N2O2S
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Color Brown to breen
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SMILES
[F-][B+3]([N]1=C(C2=CC=CS2)C=CC1=CC3=CC=C4CCCCCCCCCCCC(O)=O)([N-]34)[F-]
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (5)
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Journal Impact Factor
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Most Recent
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J Exp Clin Cancer Res
Inhibition of RACGAP1 sensitizes triple-negative breast cancer cells to ferroptosis by regulating CPT1A-dependent fatty acid metabolism. [Abstract]2025 Dec 24;44(1):323. PMID: 41444950
BODIPY 558/568 C12 purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2025 Dec 24;44(1):323. [Abstract]
BODIPY 558/568 C12 (100 µL; 30 min) staining showing the changes of lipid droplets deposition in MDA-MB-231 and 4T1 breast cells after RACGAP1 silencing and/or CPT1A overexpression
BODIPY 558/568 C12 purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2025 Dec 24;44(1):323. [Abstract]
BODIPY 558/568 C12 (100 µL; 30 min) staining showing the changes of lipid droplets deposition in MDA-MB-231 and 4T1 breast cells after RACGAP1 silencing.
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Adv Sci (Weinh)
MTFR2-Mediated Fission Drives Fatty Acid and Mitochondrial Co-Transfer from Hepatic Stellate Cells to Tumor Cells Fueling Oncogenesis. [Abstract]2025 Jun;12(23):e2416419. PMID: 40365837
BODIPY 558/568 C12 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jun;12(23):e2416419. [Abstract]
HSCs were incubated in complete media (CM) with BODIPY 558/568 C12 (Red C12) (1 μM; 16 h). aHSCs in TCM exhibited a marked shift of Red C12 from mitochondria to LDs, indicating storage of excess FAs in LDs, potentially for subsequent release within the TME.
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mBio
African swine fever virus hijacks lipolysis induced by chaperone-mediated autophagy to upregulate fatty acid β-oxidation and promote viral replication. [Abstract]2026 Apr 8;17(4):e0336825. PMID: 41801037 -
Tissue Cell
O-GlcNAc transferase-mediated O-GlcNAcylation of CD36 against myocardial ischemia-reperfusion injury. [Abstract]2025 Aug:95:102878. PMID: 40154105
BODIPY 558/568 C12 purchased from MedChemExpress. Usage Cited in: Tissue Cell. 2025 Aug:95:102878. [Abstract]
Lipid uptake of H9C2 cells was evaluated using flow cytometry with the BODIPY 558/568 C12 (6 μM; 1 h) dye.
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Solvant et solubilité
DMSO : 125 mg/mL (264.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (275 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Yu Y, et al. Human cytomegalovirus infection induces adipocyte-like lipogenesis through activation of sterol regulatory element binding protein 1. J Virol. 2012 Mar;86(6):2942-9. [Content Brief]
[2]. Bo Qiu, et al. BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry. Bio Protoc. 2016 Sep 5;6(17):e1912. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1169 mL | 10.5843 mL | 21.1685 mL | 52.9213 mL |
| 5 mM | 0.4234 mL | 2.1169 mL | 4.2337 mL | 10.5843 mL | |
| 10 mM | 0.2117 mL | 1.0584 mL | 2.1169 mL | 5.2921 mL | |
| 15 mM | 0.1411 mL | 0.7056 mL | 1.4112 mL | 3.5281 mL | |
| 20 mM | 0.1058 mL | 0.5292 mL | 1.0584 mL | 2.6461 mL | |
| 25 mM | 0.0847 mL | 0.4234 mL | 0.8467 mL | 2.1169 mL | |
| 30 mM | 0.0706 mL | 0.3528 mL | 0.7056 mL | 1.7640 mL | |
| 40 mM | 0.0529 mL | 0.2646 mL | 0.5292 mL | 1.3230 mL | |
| 50 mM | 0.0423 mL | 0.2117 mL | 0.4234 mL | 1.0584 mL | |
| 60 mM | 0.0353 mL | 0.1764 mL | 0.3528 mL | 0.8820 mL | |
| 80 mM | 0.0265 mL | 0.1323 mL | 0.2646 mL | 0.6615 mL | |
| 100 mM | 0.0212 mL | 0.1058 mL | 0.2117 mL | 0.5292 mL |
BODIPY 558/568 C12 Related Classifications
HY-138226 Related Classifications
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