BODIPY 558/568 C12

Name: BODIPY 558/568 C12
Brand: MedChemExpress
SKU: HY-138226
Rating: 5.0 (4 reviews)

Cat. No.: HY-138226 Purity: 98.42%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

BODIPY 558/568 C12 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells. Maximum excitation/emission wavelength: 558/568 nm.

For research use only. We do not sell to patients.

CAS No. : 158757-84-7

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of BODIPY 558/568 C12:

BODIPY 558/568 C12 (solution) In-stock

4 Publications Citing Use of MCE BODIPY 558/568 C12

Flow Cytometry

Cell Imaging/Staining

: BODIPY 558/568 C12 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May 14:e2416419. [Abstract]; Flow cytometry analysis showed the progressive transfer of FA labeled by BODIPY 558/568‐C12 (Red C12) (1 mM; 16 h) from HSCs to Huh7 cells at different time points (0, 4, 8, 16, and 24 h).

: BODIPY 558/568 C12 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May 14:e2416419. [Abstract]; HSCs were incubated in complete media (CM) with BODIPY 558/568‐C12 (Red C12) (1 mM; 16 h). Relative cellular localization of BODIPY 558/568‐C12 was quantified by Pearson's coefficient analysis.

: BODIPY 558/568 C12 purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2025 Dec 24;44(1):323. [Abstract]; BODIPY 558/568 C12 (100 µL; 30 min) staining showing the changes of lipid droplets deposition in MDA-MB-231 and 4T1 breast cells after RACGAP1 silencing and/or CPT1A overexpression

: BODIPY 558/568 C12 purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2025 Dec 24;44(1):323. [Abstract]; BODIPY 558/568 C12 (100 µL; 30 min) staining showing the changes of lipid droplets deposition in MDA-MB-231 and 4T1 breast cells after RACGAP1 silencing.

: BODIPY 558/568 C12 purchased from MedChemExpress. Usage Cited in: Tissue Cell. 2025 Mar 23:95:102878. [Abstract]; Lipid uptake of H9C2 cells was evaluated using flow cytometry with the BODIPY 558/568 C12 (6 μM; 1 h) dye.

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References
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Description

BODIPY 558/568 C12 is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells^[1]. Maximum excitation/emission wavelength: 558/568 nm^[1].

In Vitro

Guide (The following is our recommended experimental protocol. This protocol serves as a reference guide only. Specific operations should be adjusted according to your actual needs.)
1. Preparation of Hoechst solution
1. Preparation of BODIPY558/568C12 solution
1.1 Prepare the storage solution
Prepare a 10 mM solution.
1.2 Preparation of the working solution
Dilute the storage solution with preheated serum-free cell culture medium or PBS to prepare a 1-10 μM BODIPY558/568C12 working solution.
Note: Please adjust the concentration of BODIPY558/568C12 working solution according to the actual situation, and prepare it as needed.
2. Cell staining (suspended cells)
2.1 Centrifuge to collect cells, add PBS for two washes, each for 5 minutes. Cell density is 1×10⁶/mL
2.2 Add 1 mL of BODIPY558/568C12 working solution, incubate at room temperature for 5-30 minutes.
2.3 Centrifuge at 400 g for 3-4 minutes, discard the supernatant.
2.4 Add PBS for two washes, each for 5 minutes.
2.5 Resuspend the cells in 1 mL of serum-free medium or PBS, then observe using a fluorescence microscope or flow cytometer.
3. Cell staining (adherent cells)
3.1 Place adherent cells on a sterile cover glass.
3.2 Remove the cover glass from the culture medium, remove excess medium.
3.3 Add 100 μL of the dye working solution, gently shake to fully cover the cells, incubate for 5-30 minutes.
3.4 Remove the dye working solution, wash twice with culture medium, each for 5 minutes, and observe using a fluorescence microscope or flow cytometer.

Notes
1. Please adjust the concentration of BODIPY558/568C12 working solution and incubation time according to the actual situation.
2. It is recommended to conduct a positive control experiment. Place the control group cells in 30 μM oleic acid for 8 hours before proceeding with the subsequent experiments.
3. This product is only for professional scientific research use and must not be used for clinical diagnosis or treatment, nor for food or medicine.
4. To ensure your safety and health, please wear laboratory clothing and use disposable gloves during the operation.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

472.40

Formula

C₂₅H₃₁BF₂N₂O₂S

CAS No.

158757-84-7

Appearance

Solid

Color

Brown to breen

SMILES

[F-][B+3]([N]1=C(C2=CC=CS2)C=CC1=CC3=CC=C4CCCCCCCCCCCC(O)=O)([N-]34)[F-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (264.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1169 mL	10.5843 mL	21.1685 mL
5 mM	0.4234 mL	2.1169 mL	4.2337 mL
10 mM	0.2117 mL	1.0584 mL	2.1169 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.42%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (249 KB) HNMR (273 KB) RP-HPLC (250 KB) LCMS (86 KB)

Handling Instructions (2659 KB)

References

[1]. Yu Y, et al. Human cytomegalovirus infection induces adipocyte-like lipogenesis through activation of sterol regulatory element binding protein 1. J Virol. 2012 Mar;86(6):2942-9. [Content Brief]

[2]. Bo Qiu, et al. BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry. Bio Protoc. 2016 Sep 5;6(17):e1912. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1169 mL	10.5843 mL	21.1685 mL	52.9213 mL
	5 mM	0.4234 mL	2.1169 mL	4.2337 mL	10.5843 mL
	10 mM	0.2117 mL	1.0584 mL	2.1169 mL	5.2921 mL
	15 mM	0.1411 mL	0.7056 mL	1.4112 mL	3.5281 mL
	20 mM	0.1058 mL	0.5292 mL	1.0584 mL	2.6461 mL
	25 mM	0.0847 mL	0.4234 mL	0.8467 mL	2.1169 mL
	30 mM	0.0706 mL	0.3528 mL	0.7056 mL	1.7640 mL
	40 mM	0.0529 mL	0.2646 mL	0.5292 mL	1.3230 mL
	50 mM	0.0423 mL	0.2117 mL	0.4234 mL	1.0584 mL
	60 mM	0.0353 mL	0.1764 mL	0.3528 mL	0.8820 mL
	80 mM	0.0265 mL	0.1323 mL	0.2646 mL	0.6615 mL
	100 mM	0.0212 mL	0.1058 mL	0.2117 mL	0.5292 mL