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  4. PA-SiR-Halo

PA-SiR-Halo is a photoactivatable fluorophore targeting HaloTag. When bound to HaloTag, PA-SiR-Halo stabilizes its fluorescent form under physiological pH. After conjugation and photoactivation, it exhibits enhanced resistance to nucleophiles, and enables live-cell confocal imaging, fixed-cell single-molecule localization microscopy imaging, live-cell single-particle tracking, as well as live-cell single-molecule localization microscopy imaging of dynamic mitochondrial structures without the need for washing.

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PA-SiR-Halo

PA-SiR-Halo Chemical Structure

CAS No. : 2358677-26-4

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PA-SiR-Halo Related Antibodies

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Description

PA-SiR-Halo is a photoactivatable fluorophore targeting HaloTag. When bound to HaloTag, PA-SiR-Halo stabilizes its fluorescent form under physiological pH. After conjugation and photoactivation, it exhibits enhanced resistance to nucleophiles, and enables live-cell confocal imaging, fixed-cell single-molecule localization microscopy imaging, live-cell single-particle tracking, as well as live-cell single-molecule localization microscopy imaging of dynamic mitochondrial structures without the need for washing[1][2].

In Vitro

After conjugation with purified HaloTag protein, PA-SiR-Halo maintains stable fluorescence upon UV activation under physiological pH conditions, and its resistance to cysteamine-mediated fluorescence loss (EC50 = 3.1 mM) is higher than that of unconjugated PA-SiR[1].
PA-SiR-Halo (0.5 μM; 2 h) labels H2B-HaloTag in live U-2 OS cells. After UV activation, it reaches a signal-to-noise ratio as high as 32, with no pre-activation fluorescence signal and stable post-activation signal[1].
PA-SiR-Halo enables high-resolution fixed-cell single-molecule localization microscopy (SMLM) imaging of Cep41-HaloTag-labeled microtubules in U-2 OS cells, with a measured average microtubule diameter of 38.7 nm[1].
PA-SiR-Halo (0.5 μM; 1 h) enables live-cell single-particle tracking of β2AR-HaloTag in U-2 OS cells[1].
PA-SiR-Halo enables live-cell single-molecule localization microscopy (SMLM) imaging of dynamically changing outer mitochondrial membranes labeled with TOMM20-HaloTag in U-2 OS cells, resolving transient structures such as inter-mitochondrial tubules and fission events[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

PA-SiR-Halo Related Antibodies
Molecular Weight

600.31

Formula

C33H50ClN3O3Si
CAS No.
Appearance

Solid

Color

Blue to dark blue

SMILES

O=C(CC/C=C1C2=CC=C(N(C)C)C=C2[Si](C)(C)C3=CC(N(C)C)=CC=C\13)NCCOCCOCCCCCCCl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Purity & Documentation
References
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PA-SiR-Halo Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PA-SiR-Halo2358677-26-4Fluorescent Dyeouter mitochondrial membranessingle-molecule localization microscopyβ2AR-HaloTagHaloTagU-2 OS cellsmicrotubulesCep41-HaloTagH2B-HaloTagTOMM20-HaloTagmitochondrial structuresInhibitorinhibitorinhibit

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