BODIPY 493/503 methyl bromide
Based on 23 publication(s) in Google Scholar
BODIPY493/503 methyl bromide is a BODIPY dye. BODIPY dye is a small molecule dye with strong ultraviolet absorption ability, its fluorescence peak is relatively sharp, and the quantum yield is high. They are relatively insensitive to the polarity and pH of the environment and are relatively stable under different physiological conditions. Due to its structural asymmetry, BODIPY derives a variety of structural products. BODIPY lipid droplet dyes can well pass through the cell membrane into the cell, and localize the neutral lipids in the cell to specifically stain the lipid droplets, which can be used for labeling of live cells and fixed cells. Maximum excitation/emission wavelength: 493/503 nm.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 216434-81-0
- Formula: C14H16BBrF2N2
- Molecular Weight:341.01
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) BODIPY 493/503 methyl bromide
More- Bioact Mater. 2026 Mar 3:61:879-899. [Abstract]
- Autophagy. 2025 Dec;21(12):2792-2808. [Abstract]
- J Adv Res. 2025 Nov 16:S2090-1232(25)00930-0. [Abstract]
- Adv Sci (Weinh). 2025 Nov 12:e13270. [Abstract]
- Cell Death Dis. 2023 Aug 26;14(8):566. [Abstract]
- Mol Ther. 2025 May 8:S1525-0016(25)00382-X. [Abstract]
- Int J Biol Macromol. 2025 Apr:302:140531. [Abstract]
- Int J Biol Macromol. 2024 Apr 15;267(Pt 2):131645. [Abstract]
- Int J Biol Macromol. 2024 Mar 17;265(Pt 1):130816. [Abstract]
- J Pineal Res. 2026 Jul;78(4):e70154. [Abstract]
- Br J Pharmacol. 2026 Feb 20. [Abstract]
- PLoS Biol. 2026 May 21;24(5):e3003797. [Abstract]
- Cell Insight. 2026 Apr 14;5(3):100325. [Abstract]
- Commun Biol. 2025 Mar 28;8(1):514. [Abstract]
- Pharmaceuticals (Basel). 2026 Feb 8;19(2):285. [Abstract]
- Sci Rep. 2026 Apr 18;16(1):18062. [Abstract]
- Aquaculture. 2024 Jan 30, 579, 740209.
- FEBS J. 2026 Jan 12. [Abstract]
- Int J Biochem Cell Biol. 2024 Jun:171:106583. [Abstract]
- Adipocyte. 2025 Dec;14(1):2549456. [Abstract]
- Theriogenology. 2025 Oct 15:246:117555. [Abstract]
- Oncol Lett. 2025 Sep 24;30(6):551. [Abstract]
- Res Sq. 2025 Aug 08.
Customer Validation & Images
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
Biological Activity
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of BODIPY493/503 methyl bromide working solution
1.1 Preparation of the stock solution
Dissolve BODIPY493/503 methyl bromide in DMSO to obtain 10 mM of stock solution.
Note: It is recommended to store the stock solution at -20°C or -80°C, keep away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of BODIPY493/503 methyl bromide working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of BODIPY493/503 methyl bromide working solution according to the actual situation.
2. Cell staining (Suspension cells)
2.1 Centrifuge at 1000 g at 4°C for 3-5 min and then discard the supernatant. Wash twice with PBS, 5 min each time. The cell density is 1×106/mL.
2.2 Add 1 mL of working solution, and then incubate at room temperature for 5-30 min.
2.3 Centrifuge at 400 g at 4°C for 3-4 min and then discard the supernatant.
2.4 Wash twice with PBS, 5 min each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
3. Cell staining (Adherent cells)
3.1 Culture adherent cells on sterile coverslips.
3.2 Remove the coverslip from the medium and aspirate excess medium.
3.3 Add 100 μL of working solution, gently shake it to completely cover the cells, and then incubate at room temperature for 5-30 min.
3.4 Wash twice with medium, 5 min each time. Observation by fluorescence microscopy or flow cytometry.
Precautions
1. Please adjust the concentration of BODIPY493/503 methyl bromide working solution according to the actual situation.
2. Experiments suggest a positive control, incubate the control group cells with 30 μM oleic acid for 8 h and then perform subsequent experiments.
3. This product is only for R&D use, not for drug, household, or others.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
503
493
Chemical Information
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CAS No. 216434-81-0
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Appearance Solid
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Molecular Weight 341.01
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Formula C14H16BBrF2N2
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Color Brown to reddish brown
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SMILES
CC1=CC(C)=C(C(CBr)=C2C(C)=C3)[N-]1[B+3]([F-])([F-])[N]2=C3C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (23)
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Journal Impact Factor
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Most Recent
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Bioact Mater
A foam cell-targeted lipophagy restoration strategy stabilizes vulnerable atherosclerotic plaques. [Abstract]2026 Mar 3:61:879-899. PMID: 41809094 -
Autophagy
Renal tubular VMP1 protects against acute kidney injury via modulating autophagy and autophagy-independent pathway. [Abstract]2025 Dec;21(12):2792-2808. PMID: 40660473
BODIPY 493/503 methyl bromide purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Dec;21(12):2792-2808. [Abstract]
Immunofluorescence staining of LDs (BODIPY 493, green, 10 μM, 30 min) and ER tracker (red).
BODIPY 493/503 methyl bromide purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Dec;21(12):2792-2808. [Abstract]
Immunofluorescence staining of LDs (BODIPY 493, green, 10 μM, 30 min) and ER tracker (red) in PTECs from WT and vmp1-cKO mice with or without cisplatin.
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J Adv Res
Lactoferrin alleviates non-alcoholic steatohepatitis via remodeling gut microbiota to regulate serotonin-related pathways. [Abstract]2025 Nov 16:S2090-1232(25)00930-0. PMID: 41253270 -
Adv Sci (Weinh)
Alpinetin Nanoparticles Alleviate Optic Nerve Injury Induced by Acute Glaucoma via LRP1-PPARγ Mediated Regulation of Microglial Lipid Metabolism. [Abstract]2025 Nov 12:e13270. PMID: 41220285
BODIPY 493/503 methyl bromide purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 12:e13270. [Abstract]
To determine whether AlpNPs can reduce lipid accumulation, we measured LD levels in HMC3 cells using BODIPY 493/503 (15 min) staining.
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Cell Death Dis
USP14 governs CYP2E1 to promote nonalcoholic fatty liver disease through deubiquitination and stabilization of HSP90AA1. [Abstract]2023 Aug 26;14(8):566. PMID: 37633951
BODIPY 493/503 methyl bromide purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2023 Aug 26;14(8):566. [Abstract]
AML12 cells were PO-treated and transfected with the corresponding adenovirus for BODIPY (10 μM, 30 min) staining (n = 3), scale bar, 200 μm.
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Mol Ther
Renal tubular epithelial IGFBP7 interacts with PKM2 to drive renal lipid accumulation and fibrosis. [Abstract]2025 May 8:S1525-0016(25)00382-X. PMID: 40346800 -
Int J Biol Macromol
FGF1 alleviates polystyrene nanoplastics-induced neuroinflammation through the suppression of lipophagy. [Abstract]2025 Apr:302:140531. PMID: 39892539 -
Int J Biol Macromol
Mechanistic insights into the role of USP14 in adipose tissue macrophage recruitment and insulin resistance in obesity. [Abstract]2024 Apr 15;267(Pt 2):131645. PMID: 38631582 -
Int J Biol Macromol
Dihydrolipoyl dehydrogenase promotes white adipocytes browning by activating the RAS/ERK pathway and undergoing crotonylation modification. [Abstract]2024 Mar 17;265(Pt 1):130816. PMID: 38503371 -
J Pineal Res
Circadian-Related Serotonin/Melatonin Level Modulates Cisplatin Ototoxicity Susceptibility Depended on NOS3-NO Pathway. [Abstract]2026 Jul;78(4):e70154. PMID: 42262169 -
Br J Pharmacol
2026 Feb 20. PMID: 41717688 -
PLoS Biol
A non-canonical JAK/STAT pathway promotes viral replication through the lipoprotein receptor-related protein in ticks. [Abstract]2026 May 21;24(5):e3003797. PMID: 42166417 -
Cell Insight
Identification of Foxm1 as a critical regulator for metabolic dysfunction-associated steatotic liver disease by epigenomic and transcriptional profiling. [Abstract]2026 Apr 14;5(3):100325. PMID: 42088639 -
Commun Biol
Defining gastric cancer ecology: the crucial roles of TREM2+ macrophages and fibroblasts in tumor microenvironments. [Abstract]2025 Mar 28;8(1):514. PMID: 40155473 -
Pharmaceuticals (Basel)
IL-33-Driven Macrophage Reprogramming as a Potential Immunometabolic Strategy for Herpes Simplex Keratitis. [Abstract]2026 Feb 8;19(2):285. PMID: 41754825 -
Sci Rep
Small-molecule FTO inhibitor MO-I-500 protects C8-B4 microglial cells from erastin-induced ferroptosis. [Abstract]2026 Apr 18;16(1):18062. PMID: 42000801 -
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FEBS J
Mitochondrial uncoupler BAM15 ameliorates liver lipid metabolism disorders by activating the AMPK pathway. [Abstract]2026 Jan 12. PMID: 41527408 -
Int J Biochem Cell Biol
NDUFA9 and its crotonylation modification promote browning of white adipocytes by activating mitochondrial function in mice. [Abstract]2024 Jun:171:106583. PMID: 38657899 -
Adipocyte
2025 Dec;14(1):2549456. PMID: 41381093
BODIPY 493/503 methyl bromide purchased from MedChemExpress. Usage Cited in: Adipocyte. 2025 Dec;14(1):2549456. [Abstract]
BODIPY (10 μM, 15 min) staining revealed that while LA treatment markedly enhanced lipid accumulation, Capg knockdown significantly attenuated adipogenesis.
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Theriogenology
Procyanidin B2 improves the in vitro maturation of porcine oocytes and subsequent embryonic development. [Abstract]2025 Oct 15:246:117555. PMID: 40580853 -
Oncol Lett
2025 Sep 24;30(6):551. PMID: 41078715 -
Solvent & Solubility
DMSO : 10 mg/mL (29.32 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (287 KB)
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SDS (616 KB)
- English - EN (616 KB)
- Français - FR (616 KB)
- Deutsch - DE (616 KB)
- Norwegian - NO (616 KB)
- Español - ES (616 KB)
- Swedish - SV (616 KB)
- Italian - IT (616 KB)
- Korean - KR (616 KB)
- Portuguese - PT (616 KB)
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Handling Instructions (2659 KB)
References
[1]. Zaiguo Li, et al. Synthesis and spectral properties of cholesterol- and FTY720-containing boron dipyrromethene dyes. Synthesis and spectral properties of cholesterol- and FTY720-containing boron dipyrromethene dyes. J Org Chem. 2007 Oct 26;72(22):8376-82. [Content Brief]
[2]. Bo Qiu, et al. BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry. Bio Protoc. 2016 Sep 5;6(17):e1912. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9325 mL | 14.6623 mL | 29.3247 mL | 73.3116 mL |
| 5 mM | 0.5865 mL | 2.9325 mL | 5.8649 mL | 14.6623 mL | |
| 10 mM | 0.2932 mL | 1.4662 mL | 2.9325 mL | 7.3312 mL | |
| 15 mM | 0.1955 mL | 0.9775 mL | 1.9550 mL | 4.8874 mL | |
| 20 mM | 0.1466 mL | 0.7331 mL | 1.4662 mL | 3.6656 mL | |
| 25 mM | 0.1173 mL | 0.5865 mL | 1.1730 mL | 2.9325 mL |
BODIPY 493/503 methyl bromide Related Classifications
HY-D1614 Related Classifications
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