SPY555-BG
SPY555-BG (SNAP-MaP555) is a cell-permeable fluorescent dye for SNAP-tag labeling of HIV-1 Gag, which enables visualization of HIV-1 intercellular transmission via SNAPf. SPY555-BG relies on irreversible covalent binding to the active-site cysteine of the SNAPf tag fused to HIV-1 Gag.
For research use only. We do not sell to patients.
- CAS No.: 2434651-49-5
- Formula: C40H40N10O6S
- Molecular Weight:788.87
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Operating Instructions
(The following is a recommended experimental protocol for guidance only, and adjustments are required based on your specific needs)
1. Staining Procedure
1.1 Sample type: Suspended T cells (donor cell population expressing Gag.SNAPf)[1].
1.2 Incubation conditions: Use cell-permeable SPY555-BG (benzylguanine-conjugated SNAP dye) to label Gag.SNAPf in donor cells.
1.3 Washing steps: (No relevant valid information is available in this section, so it is omitted entirely)
2. Detection and Analysis
2.1 Instrument: Spinning disk confocal microscope or live-cell imaging system.
2.2 Result analysis:
2.2.1 Observe the localization of Gag.SNAPf at the virological synapses formed between donor and target T cells.
2.2.2 Track the de novo expression of Gag.SNAPf in target cells after virological synapse formation.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2434651-49-5
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Molecular Weight 788.87
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Formula C40H40N10O6S
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SMILES
CN(C)C1=CC2=C(C3(N(S(=O)(N(C)C)=O)C(C4=C3C=C(C(NCC5=CC=C(COC6=C(N=CN7)C7=NC(N)=N6)C=C5)=O)C=C4)=O)C(C=CC(N(C)C)=C8)=C8O2)C=C1
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Synonyms
SNAP-MaP555
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
SPY555-BG Related Classifications
HY-D2994 Related Classifications
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