SPY555-BG
Based on 1 Customer Validation
SPY555-BG (SNAP-MaP555) is a cell-permeable fluorescent dye for SNAP-tag labeling of HIV-1 Gag, which enables visualization of HIV-1 intercellular transmission via SNAPf. SPY555-BG relies on irreversible covalent binding to the active-site cysteine of the SNAPf tag fused to HIV-1 Gag.
For research use only. We do not sell to patients.
- CAS No.: 2434651-49-5
- Formula: C40H40N10O6S
- Molecular Weight:788.87
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Operating Instructions
(The following is a recommended experimental protocol for guidance only, and adjustments are required based on your specific needs)
1. Staining Procedure
1.1 Sample type: Suspended T cells (donor cell population expressing Gag.SNAPf)[1].
1.2 Incubation conditions: Use cell-permeable SPY555-BG (benzylguanine-conjugated SNAP dye) to label Gag.SNAPf in donor cells.
1.3 Washing steps: (No relevant valid information is available in this section, so it is omitted entirely)
2. Detection and Analysis
2.1 Instrument: Spinning disk confocal microscope or live-cell imaging system.
2.2 Result analysis:
2.2.1 Observe the localization of Gag.SNAPf at the virological synapses formed between donor and target T cells.
2.2.2 Track the de novo expression of Gag.SNAPf in target cells after virological synapse formation.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
Chemical Information
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CAS No. 2434651-49-5
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Appearance Solid
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Molecular Weight 788.87
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Formula C40H40N10O6S
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SMILES
CN(C)C1=CC2=C(C3(N(S(=O)(N(C)C)=O)C(C4=C3C=C(C(NCC5=CC=C(COC6=C(N=CN7)C7=NC(N)=N6)C=C5)=O)C=C4)=O)C(C=CC(N(C)C)=C8)=C8O2)C=C1
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Synonyms
SNAP-MaP555
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (126.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2676 mL | 6.3382 mL | 12.6764 mL | 31.6909 mL |
| 5 mM | 0.2535 mL | 1.2676 mL | 2.5353 mL | 6.3382 mL | |
| 10 mM | 0.1268 mL | 0.6338 mL | 1.2676 mL | 3.1691 mL | |
| 15 mM | 0.0845 mL | 0.4225 mL | 0.8451 mL | 2.1127 mL | |
| 20 mM | 0.0634 mL | 0.3169 mL | 0.6338 mL | 1.5845 mL | |
| 25 mM | 0.0507 mL | 0.2535 mL | 0.5071 mL | 1.2676 mL | |
| 30 mM | 0.0423 mL | 0.2113 mL | 0.4225 mL | 1.0564 mL | |
| 40 mM | 0.0317 mL | 0.1585 mL | 0.3169 mL | 0.7923 mL | |
| 50 mM | 0.0254 mL | 0.1268 mL | 0.2535 mL | 0.6338 mL | |
| 60 mM | 0.0211 mL | 0.1056 mL | 0.2113 mL | 0.5282 mL | |
| 80 mM | 0.0158 mL | 0.0792 mL | 0.1585 mL | 0.3961 mL | |
| 100 mM | 0.0127 mL | 0.0634 mL | 0.1268 mL | 0.3169 mL |
SPY555-BG Related Classifications
HY-D2994 Related Classifications
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