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  4. Sulfo-Cy5-TCO

Sulfo-Cy5-TCO is a water-soluble sulfonated Cy5 fluorescent reagent containing trans-cyclooctene (TCO). Sulfo-Cy5-TCO undergoes an inverse electron demand Diels‑Alder reaction with tetrazine-labeled fixed breast cancer cells to achieve cell labeling. Sulfo-Cy5-TCO is used for fluorescent labeling and imaging of biological samples such as proteins and cells.

For research use only. We do not sell to patients.

Sulfo-Cy5-TCO

Sulfo-Cy5-TCO Chemical Structure

CAS No. : 2129525-69-3

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Sulfo-Cy5-TCO Related Antibodies

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Description

Sulfo-Cy5-TCO is a water-soluble sulfonated Cy5 fluorescent reagent containing trans-cyclooctene (TCO). Sulfo-Cy5-TCO undergoes an inverse electron demand Diels‑Alder reaction with tetrazine-labeled fixed breast cancer cells to achieve cell labeling. Sulfo-Cy5-TCO is used for fluorescent labeling and imaging of biological samples such as proteins and cells[1].

In Vitro

Sulfo-Cy5-TCO undergoes an iEDDA reaction with tetrazine-modified BSA in ammonium acetate buffer at 25 °C to form a fluorescent conjugate, with a yield of approximately 50%[1].
Sulfo-Cy5-TCO can specifically label immobilized MCF7 breast cancer cells with tetrazine tags via the iEDDA reaction, generating detectable red fluorescence, and no non-specific binding is observed under control conditions[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Sulfo-Cy5-TCO Related Antibodies
Molecular Weight

959.20

Formula

C46H62N4O12S3
CAS No.
Emission (Em)

670
Excitation (Ex)

633
SMILES

O=C(NCCCNC(CCCCCN1C2=CC=C(S(=O)(O)=O)C=C2C(C)(C)/C1=C\C=C\C=C\C3=[N+](CCCS(=O)([O-])=O)C4=C(C3(C)C)C=C(S(=O)(O)=O)C=C4)=O)OC5CC/C=C\CCC5
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Sulfo-Cy5-TCO Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sulfo-Cy5-TCO2129525-69-3Fluorescent Dyecell labellingMCF7 breast cancer cellsinverse electron demand Diels-Alder reactionsbovine serum albumintetrazine-functionalized biomoleculesfixed breast cancer cellsiEDDA reactionammonium acetate bufferbio-orthogonal conjugationInhibitorinhibitorinhibit

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