2. JAK/STAT Signaling Stem Cell/Wnt Protein Tyrosine Kinase/RTK Epigenetics Anti-infection
  3. STAT JAK HSV
  4. 2-NP

2-NP is a selective enhancer of STAT1 transcription. 2-NP can enhance the ability of IFN-γ to inhibit the proliferation of human breast cancer and fibrosarcoma cells.

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CAS 番号 : 65182-56-1

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 77 在庫あり
Solution
10 mM * 1 mL in DMSO USD 77 在庫あり
Solid
1 mg $33 在庫あり
5 mg $70 在庫あり
10 mg $120 在庫あり
25 mg $240 在庫あり
50 mg $380 在庫あり
100 mg $610 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 13 publication(s) in Google Scholar

2-NP 関連抗体

Top Publications Citing Use of Products

    2-NP purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 23:e04569.  [Abstract]

    2-NP (45 × 10−6 M; 2 h) induced enhancement of STAT1 phosphorylation in PBMC-derived macrophages.

    2-NP purchased from MedChemExpress. Usage Cited in: Phytother Res. 2025 Aug 12.  [Abstract]

    2-NP (20 mg/kg; i.g.; once daily for 3 d) aggravated the disordered hepatocytes and inflammatory cell infiltration in CLP (cecal ligation and puncture) mice.

    2-NP purchased from MedChemExpress. Usage Cited in: Phytother Res. 2025 Aug 12.  [Abstract]

    2-NP (20 mg/kg; i.g.; once daily for 3 d) diminished therapeutic effect of COP on inflammatory factors evidenced by the detection of IL-6 and TNF-α in the serums of CLP (cecal ligation and puncture) mice.

    2-NP purchased from MedChemExpress. Usage Cited in: Phytother Res. 2025 Aug 12.  [Abstract]

    2-NP (20 mg/kg; i.g.; once daily for 3 d) diminished the effect of COP on reducing MDA and increasing GSH in liver tissues in the serums of CLP (cecal ligation and puncture) mice.

    2-NP purchased from MedChemExpress. Usage Cited in: Phytother Res. 2025 Aug 12.  [Abstract]

    2-NP (20 mg/kg; i.g.; once daily for 3 d) weakened therapeutic effect of COP on restoring GPX4 and inhibiting the phosphorylation of STAT1 and IRF1 in CLP (cecal ligation and puncture) mice.

    STAT アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    STAT3 STAT5 STAT6 STAT1

    JAK アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    JAK1 JAK2 JAK3 Tyk2 JAK

    HSV アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    HSV-1 HSV-2 HSV

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    2-NP is a selective enhancer of STAT1 transcription. 2-NP can enhance the ability of IFN-γ to inhibit the proliferation of human breast cancer and fibrosarcoma cells[1].

    体外実験

    2-NP (5 μmol/mL, 24 h) inhibits the viral replication of Duck plague virus (DPV) through activation of the JAK-STAT signaling pathway in duck embryo fibroblasts (DEF)[2].
    2-NP (45 μM, 48 h) promotes the expression of CXCR3 in mouse plasmacytoid dendritic cells (pDCs), promotes CXCL10-induced pDC migration[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    2-NP 関連抗体

    Western Blot Analysis[3]

    Cell Line: mouse plasmacytoid dendritic cell
    Concentration: 45 μM
    Incubation Time: 48 h
    Result: Increased the phosphorylation of JAK1 and STAT1.

    Cell Migration Assay [3]

    Cell Line: mouse plasmacytoid dendritic cell
    Concentration: 45 μM
    Incubation Time: 48 h
    Result: Promoted the migration of pDCs.
    体内実験

    2-NP (0.5 mg/kg, subconjunctival injection, once daily for 3 days) alleviates corneal lesions and exhibits antiviral activity in Herpes simplex keratitis (HSK) mouse model[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Herpes simplex keratitis (HSK) model[3]
    Dosage: 0.5 mg/kg
    Administration: subconjunctival injection, once daily for 3 days
    Result: Alleviated corneal lesions in mice, reduced corneal opacity and neovascularization.
    分子量

    222.25

    分子式

    C14H10N2O
    CAS 番号
    Appearance

    Solid

    Color

    Yellow to orange

    SMILES

    OC1=CC=CC=C1C2=NC3=NC=CC=C3C=C2
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
    溶剤 & 溶解度
    体外: 

    DMSO : 50 mg/mL (224.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.4994 mL 22.4972 mL 44.9944 mL
    5 mM 0.8999 mL 4.4994 mL 8.9989 mL
    10 mM 0.4499 mL 2.2497 mL 4.4994 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

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    体積 （開始）

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    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 1.25 mg/mL (5.62 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    +
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.4994 mL 22.4972 mL 44.9944 mL 112.4859 mL
    5 mM 0.8999 mL 4.4994 mL 8.9989 mL 22.4972 mL
    10 mM 0.4499 mL 2.2497 mL 4.4994 mL 11.2486 mL
    15 mM 0.3000 mL 1.4998 mL 2.9996 mL 7.4991 mL
    20 mM 0.2250 mL 1.1249 mL 2.2497 mL 5.6243 mL
    25 mM 0.1800 mL 0.8999 mL 1.7998 mL 4.4994 mL
    30 mM 0.1500 mL 0.7499 mL 1.4998 mL 3.7495 mL
    40 mM 0.1125 mL 0.5624 mL 1.1249 mL 2.8121 mL
    50 mM 0.0900 mL 0.4499 mL 0.8999 mL 2.2497 mL
    60 mM 0.0750 mL 0.3750 mL 0.7499 mL 1.8748 mL
    80 mM 0.0562 mL 0.2812 mL 0.5624 mL 1.4061 mL
    100 mM 0.0450 mL 0.2250 mL 0.4499 mL 1.1249 mL
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    2-NP Related Classifications

    • Molarity Calculator

    • Dilution Calculator

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    2-NP65182-56-1STATJAKHSVJanus kinaseHerpes simplex virusbreast cancerfibrosarcomaC57BL/6J mouseInhibitorinhibitorinhibit

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    製品名:
    2-NP
    製品番号:
    HY-W013523
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